Universitą degli studi di Pavia
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Astesana's curriculum
Curriculum
PERSONAL INFORMATION
Name: VALENTINA ASTESANA
Date of Birth: 09/08/1987 - Savigliano (CN)
Nationality: Italian
e-mail: valentina.astesana01@universitadipavia.it
WORK EXPERIENCE
October 2015- onwards
PhD student in Genetics, Molecular and Cellular Biology
Cellular Biology and Neurobiology Laboratory – Department of Biology and Biotechnology “L. Spallanzani” - University of Pavia, Via A. Ferrata 9 – 27100 Pavia – Italy.
January 2015- June 2015
Volunteer
ARCO Foundation- Cancer Genetics and Translational Oncology Laboratory of S. Croce General Hospital, Cuneo; Via Michele Coppino, 26 – 12100 Cuneo – Italy. Lab supervisor: Dr. M. Merlano
Project title: “Analysis of specific polymorphisms in genes involved in chemo-drug metabolism associated with increased risk of treatment-induced toxicity or resistance by DNA sequencing and pyrosequencing”
Project title: “Detection of polymorphisms and prognostic factor in head and neck cancer and in colon cancer”
June 2014 - December 2014
Volunteer
Cancer Genetics and Translational Oncology Laboratory of S. Croce General Hospital, Cuneo; Via Michele Coppino, 26 – Cuneo – Italy. Lab supervisor: Dr. M. Merlano
Project title: Reoxygenation reverse hypoxia-related radioresistance in head and neck cancer cell lines.
September 2011 – May 2013
Internships
Cell Therapy Laboratory, Institute of Cancer Research and Treatment (IRCCS) Strada Provinciale 142, 10060 Candiolo (TO) – Italy. Lab supervisor: Dr Giorgio Valabrega
Thesis project: “Adoptive immunotherapy in the treatment of ERBB2 positive breast cancer sensitive and resistant to Trastuzumab”
May 2010 - December 2010
Internships
Department of Drug Science and Technology – University of Turin, Via P. Giuria, 9 - 10125 Torino – Italy. Lab supervisor: Dr. Roberto Canaparo
Thesis project: “Development and validation of an HPLC method for the separation of the enantiomers of the β-blocker carvedilol in human plasma”
EDUCATION AND TRAINING
2013 - Professional habilitation in Biology – University of Pavia
2010-2013 - Master of Science in Cellular and Molecular Biology – University of Turin – 108/110
2007-2010 - Bachelor of Science in Biology - University of Turin – 100/110
TECHNICAL SKILLS
Cell cultures, manipulation of biological samples (blood, serum, fresh and paraffin tissue)
In vitro treatment with drugs, proliferation tests
PCR, electrophoresis on agarose gel
Extraction and quantification of DNA and RNA, extraction and quantification of proteins, WB
Sanger’s sequencing, Pyrosequencing
COMPUTER SKILLS
ECDL Full
SOCIAL SKILLS AND COMPETENCES
Ability to organize own work, setting priorities and taking the responsibility to respect deadlines and objectives. Ability to work in a profitable way, be independent of sharing spaces and common rules
LANGUAGE SKILLS
Italian: Mother tongue
English: Intermediate level
French: Intermediate level
ATTENDANCE TO MEETINGS AND COURSES
Conferences: M.A.C.C. 9 – March 20th-21st 2015 – Saint Paul de Vence – Nice- France
Courses: - 5th national course AIOM and SIAPEC-IAP – From laboratory to clinic: new biomarkers - May 13, 2015 – Genoa – Italy
Publications
Abstracts:
Lo Nigro C, Vivenza D, Monteverde M, Fortunato M, Denaro N, Lattanzio L, Tonissi F, Astesana V, Russi E, Merlano M “ Prognostic value of HPV detection with three primer sets in 255 Head-Neck cancers” – 2015 ASCO Annual Meeting - 29Maggio -2 Giungo, 2015 - Chicago
Research Project
Antitumour Platinum compounds on neural cell lines. Microscopical and biomolecular analysis
The research activity is addressed to the study of cellular and molecular effects induced by cisplatin and a new platinum compound, the [Pt(O,O'-acac)(γ-acac)(DMS)], on human and rat neuroblastoma and glioblastoma cell lines. In particular relevance will be given to the study of cellular accumulation of the two compounds, new cellular pathways triggered, effects on intracellular calcium homeostasis, interaction with the blood-brain barrier and epigenetic mechanisms involved in drug-resistance (miRNAs).
PtAcacDMS is stable under physiological conditions, acts on the regulation of intracellular calcium and, compared to platinum, it displays a low neurotoxicity in normal tissues and a low reactivity with DNA, suggesting a possible interaction with non-genomic targets, such as proteins involved in key pathways of cell metabolism.
PERSONAL INFORMATION
Name: VALENTINA ASTESANA
Date of Birth: 09/08/1987 - Savigliano (CN)
Nationality: Italian
e-mail: valentina.astesana01@universitadipavia.it
WORK EXPERIENCE
October 2015- onwards
PhD student in Genetics, Molecular and Cellular Biology
Cellular Biology and Neurobiology Laboratory – Department of Biology and Biotechnology “L. Spallanzani” - University of Pavia, Via A. Ferrata 9 – 27100 Pavia – Italy.
January 2015- June 2015
Volunteer
ARCO Foundation- Cancer Genetics and Translational Oncology Laboratory of S. Croce General Hospital, Cuneo; Via Michele Coppino, 26 – 12100 Cuneo – Italy. Lab supervisor: Dr. M. Merlano
Project title: “Analysis of specific polymorphisms in genes involved in chemo-drug metabolism associated with increased risk of treatment-induced toxicity or resistance by DNA sequencing and pyrosequencing”
Project title: “Detection of polymorphisms and prognostic factor in head and neck cancer and in colon cancer”
June 2014 - December 2014
Volunteer
Cancer Genetics and Translational Oncology Laboratory of S. Croce General Hospital, Cuneo; Via Michele Coppino, 26 – Cuneo – Italy. Lab supervisor: Dr. M. Merlano
Project title: Reoxygenation reverse hypoxia-related radioresistance in head and neck cancer cell lines.
September 2011 – May 2013
Internships
Cell Therapy Laboratory, Institute of Cancer Research and Treatment (IRCCS) Strada Provinciale 142, 10060 Candiolo (TO) – Italy. Lab supervisor: Dr Giorgio Valabrega
Thesis project: “Adoptive immunotherapy in the treatment of ERBB2 positive breast cancer sensitive and resistant to Trastuzumab”
May 2010 - December 2010
Internships
Department of Drug Science and Technology – University of Turin, Via P. Giuria, 9 - 10125 Torino – Italy. Lab supervisor: Dr. Roberto Canaparo
Thesis project: “Development and validation of an HPLC method for the separation of the enantiomers of the β-blocker carvedilol in human plasma”
EDUCATION AND TRAINING
2013 - Professional habilitation in Biology – University of Pavia
2010-2013 - Master of Science in Cellular and Molecular Biology – University of Turin – 108/110
2007-2010 - Bachelor of Science in Biology - University of Turin – 100/110
TECHNICAL SKILLS
Cell cultures, manipulation of biological samples (blood, serum, fresh and paraffin tissue)
In vitro treatment with drugs, proliferation tests
PCR, electrophoresis on agarose gel
Extraction and quantification of DNA and RNA, extraction and quantification of proteins, WB
Sanger’s sequencing, Pyrosequencing
COMPUTER SKILLS
ECDL Full
SOCIAL SKILLS AND COMPETENCES
Ability to organize own work, setting priorities and taking the responsibility to respect deadlines and objectives. Ability to work in a profitable way, be independent of sharing spaces and common rules
LANGUAGE SKILLS
Italian: Mother tongue
English: Intermediate level
French: Intermediate level
ATTENDANCE TO MEETINGS AND COURSES
Conferences: M.A.C.C. 9 – March 20th-21st 2015 – Saint Paul de Vence – Nice- France
Courses: - 5th national course AIOM and SIAPEC-IAP – From laboratory to clinic: new biomarkers - May 13, 2015 – Genoa – Italy
Publications
Abstracts:
Lo Nigro C, Vivenza D, Monteverde M, Fortunato M, Denaro N, Lattanzio L, Tonissi F, Astesana V, Russi E, Merlano M “ Prognostic value of HPV detection with three primer sets in 255 Head-Neck cancers” – 2015 ASCO Annual Meeting - 29Maggio -2 Giungo, 2015 - Chicago
Research Project
Antitumour Platinum compounds on neural cell lines. Microscopical and biomolecular analysis
The research activity is addressed to the study of cellular and molecular effects induced by cisplatin and a new platinum compound, the [Pt(O,O'-acac)(γ-acac)(DMS)], on human and rat neuroblastoma and glioblastoma cell lines. In particular relevance will be given to the study of cellular accumulation of the two compounds, new cellular pathways triggered, effects on intracellular calcium homeostasis, interaction with the blood-brain barrier and epigenetic mechanisms involved in drug-resistance (miRNAs).
PtAcacDMS is stable under physiological conditions, acts on the regulation of intracellular calcium and, compared to platinum, it displays a low neurotoxicity in normal tissues and a low reactivity with DNA, suggesting a possible interaction with non-genomic targets, such as proteins involved in key pathways of cell metabolism.
