Università degli studi di Pavia
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Mentegari's curriculum
Curriculum
PERSONAL DATA
ELISA MENTEGARI
born 28/11/1991
elisa.mentegari@gmail.com
Driving licence B, car availability
EDUCATION
• From October 2015 - PhD program in Genetics, cellular and molecular Biology. University of Pavia.
• September 2015 - Laurea magistralis in Molecular Biology and Genetics. University of Pavia. Thesis title: “Ribonucleotide incorporation by human specialized DNA polymerases opposite 7,8-dihydro-8-oxoguanine (8-oxo-G) lesion”. Mark: 110/110 cum laude.
• July, 2013 - Bachelor’s degree in biology (molecular and genetic orientation). University of Pavia. Thesis title: “Analisi elettrofisiologica delle correnti di sodio nelle cellule tsA-201 dopo espressione del canale Nav1.1 umano”. Mark: 105/110.
• July, 2010 –High school diploma. Second level college of science Niccolò Copernico (Pavia). Mark: 95/100.
WORKING EXPERIENCE
• SEPTEMBER 2013 – SEPTEMBER 2015
Laboratory of DNA enzymology and molecular virology, directed by Dr. Maga. Institute of
Molecular Genetics – CNR (Pavia).
‒ In these two years I have been involved in different projects. The one I worked on for my experimental thesis deals with the molecular and biochemical characterization of human specialized polymerases and . In particular, we focused our attention on incorporation of ribonucleotides opposite 7,8-dihydro-8-oxoguanine (8-oxo-G), one of the most relevant and widely distributed DNA oxidative damages.
• NOVEMBER 2012 - JULY 2013
Laboratory of electrophysiology and biophysics of neuronal ionic channels, directed by Dr. Toselli. Department of biology and biotechnology “Lazzaro Spallanzani” (Pavia).
At the last year of my bachelor’s degree I participated to a project aimed at expressing human ionic channels in mammalian cells; elettrophysiological analysis of sodium currents passing through them were performed.
COMPETENCES
• Mother tongue: ITALIAN
• Other languages: ENGLISH - C1 / FRENCH - A1 / SPANISH - A1
• Professional competences:
‒ Enzymatic assays: polymerase assay, helicase assay, evaluation of DNA intercalating agents, inhibition assays.
‒ Polyacrylamide/agarose/SDS-PAGE/2D SDS-PAGE gels
‒ Cloning
‒ Cell cultures
‒ Plasmid purification (mini/midi-prep)
‒ Transformation (heat shock/electroporation)
‒ E. Coli protein expression and purification (FPLC, batch purification)
‒ Western blot
‒ Quantitation of DNA and proteins
‒ PCR/ Real-Time PCR
‒ Use of different lab instruments: electrophoresis devices (polyacrylamide/agarose gels), spectrophotometers, CHEMI DOC XRS, typhoon laser scanner, beta-counter, Real-time PCR instruments, FPLC system, super- and ultra-centrifuges.
• Informatic skills:
‒ Excellent use of Microsoft Office Word and Power Point
‒ Adequate use of imaging programs (Photoshop)
‒ Use of the following databases: NCBI (Pubmed, Gene, Genome, GEO, Nucleotide, OMIM), Uniprot (Blast), UCSC (Blast/Blat, Genome Browser)
‒ Use of social networks (Linkedin)
Publications
Crespan E, Furrer A, Rösinger M, Bertoletti F, Mentegari E, Chiapparini G, Imhof R, Ziegler N, Sturla SJ, Hübscher U, van Loon B, Maga G. Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair. Nat Commun. 2016 Feb 26;7:10805. doi: 10.1038/ncomms10805. PubMed PMID: 26917111; PubMed Central PMCID: PMC4773436.
Research project
Role of reparative DNA polymerases and their post-translational modifications in pathways activated following genotoxic stress
Damages on DNA can arise from both endogenous and exogenous sources. Genome integrity is critical for DNA replication and cell survival. For this reason, cells have evolved different strategies to cope with possible DNA damages. Reparative DNA polymerases play a fundamental role in DNA repair pathways.
The aim of my research project is to characterize the role of these enzymes in different pathways. In particular, my attention will be focused on the influence of post-translational modifications, occurring at DNA pols, which take place in response to genotoxic stress in different model organisms.
PERSONAL DATA
ELISA MENTEGARI
born 28/11/1991
elisa.mentegari@gmail.com
Driving licence B, car availability
EDUCATION
• From October 2015 - PhD program in Genetics, cellular and molecular Biology. University of Pavia.
• September 2015 - Laurea magistralis in Molecular Biology and Genetics. University of Pavia. Thesis title: “Ribonucleotide incorporation by human specialized DNA polymerases opposite 7,8-dihydro-8-oxoguanine (8-oxo-G) lesion”. Mark: 110/110 cum laude.
• July, 2013 - Bachelor’s degree in biology (molecular and genetic orientation). University of Pavia. Thesis title: “Analisi elettrofisiologica delle correnti di sodio nelle cellule tsA-201 dopo espressione del canale Nav1.1 umano”. Mark: 105/110.
• July, 2010 –High school diploma. Second level college of science Niccolò Copernico (Pavia). Mark: 95/100.
WORKING EXPERIENCE
• SEPTEMBER 2013 – SEPTEMBER 2015
Laboratory of DNA enzymology and molecular virology, directed by Dr. Maga. Institute of
Molecular Genetics – CNR (Pavia).
‒ In these two years I have been involved in different projects. The one I worked on for my experimental thesis deals with the molecular and biochemical characterization of human specialized polymerases and . In particular, we focused our attention on incorporation of ribonucleotides opposite 7,8-dihydro-8-oxoguanine (8-oxo-G), one of the most relevant and widely distributed DNA oxidative damages.
• NOVEMBER 2012 - JULY 2013
Laboratory of electrophysiology and biophysics of neuronal ionic channels, directed by Dr. Toselli. Department of biology and biotechnology “Lazzaro Spallanzani” (Pavia).
At the last year of my bachelor’s degree I participated to a project aimed at expressing human ionic channels in mammalian cells; elettrophysiological analysis of sodium currents passing through them were performed.
COMPETENCES
• Mother tongue: ITALIAN
• Other languages: ENGLISH - C1 / FRENCH - A1 / SPANISH - A1
• Professional competences:
‒ Enzymatic assays: polymerase assay, helicase assay, evaluation of DNA intercalating agents, inhibition assays.
‒ Polyacrylamide/agarose/SDS-PAGE/2D SDS-PAGE gels
‒ Cloning
‒ Cell cultures
‒ Plasmid purification (mini/midi-prep)
‒ Transformation (heat shock/electroporation)
‒ E. Coli protein expression and purification (FPLC, batch purification)
‒ Western blot
‒ Quantitation of DNA and proteins
‒ PCR/ Real-Time PCR
‒ Use of different lab instruments: electrophoresis devices (polyacrylamide/agarose gels), spectrophotometers, CHEMI DOC XRS, typhoon laser scanner, beta-counter, Real-time PCR instruments, FPLC system, super- and ultra-centrifuges.
• Informatic skills:
‒ Excellent use of Microsoft Office Word and Power Point
‒ Adequate use of imaging programs (Photoshop)
‒ Use of the following databases: NCBI (Pubmed, Gene, Genome, GEO, Nucleotide, OMIM), Uniprot (Blast), UCSC (Blast/Blat, Genome Browser)
‒ Use of social networks (Linkedin)
Publications
Crespan E, Furrer A, Rösinger M, Bertoletti F, Mentegari E, Chiapparini G, Imhof R, Ziegler N, Sturla SJ, Hübscher U, van Loon B, Maga G. Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair. Nat Commun. 2016 Feb 26;7:10805. doi: 10.1038/ncomms10805. PubMed PMID: 26917111; PubMed Central PMCID: PMC4773436.
Research project
Role of reparative DNA polymerases and their post-translational modifications in pathways activated following genotoxic stress
Damages on DNA can arise from both endogenous and exogenous sources. Genome integrity is critical for DNA replication and cell survival. For this reason, cells have evolved different strategies to cope with possible DNA damages. Reparative DNA polymerases play a fundamental role in DNA repair pathways.
The aim of my research project is to characterize the role of these enzymes in different pathways. In particular, my attention will be focused on the influence of post-translational modifications, occurring at DNA pols, which take place in response to genotoxic stress in different model organisms.
