Universitą degli studi di Pavia
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Masiello's curriculum
Curriculum
PERSONAL INFORMATION
First Name and Surname: Irene Masiello;
Address: Via Maiorino 43, 04023 Formia (LT – Italy);
Telephon: 0771 770364;
Mobile: 328 0639451;
E-mail: irene.masiello@virgilio.it;
Date of Birth: 24/10/1989;
Nationality: Italian.
EDUCATION AND TRAINING
• Title of qualification awarded: Master’s Degree in Biological Sciences (Molecular Diagnostics);
Date: 26/03/2014;
Name and type of organization providing education and training: University “Federico II” of Naples – Faculty of MM. FF. NN. Sciences;
Thesis: Experimental thesis in Molecular Biology – Antibody production for p53 e mdm-like proteins of Mytilus galloprovincialis;
Vote: 110/110 cum laude.
• Title of qualification awarded: Bachelor’s Degree in Biological Sciences;
Date: 23/02/2012;
Name and type of organization providing education and training: University of Naples “Federico II” – Faculty of MM. FF. NN. Sciences;
Thesis: Experimental thesis in Molecular Biology – Danio rerio RPLP0 cloning in an expression vector;
Vote: 110/110 cum laude.
• Title of qualification awarded: High School Qualification in classical studies;
Date: 2008;
Name and type of organization providing education and training: Liceo Classico Vitruvio Pollione – Formia;
Vote: 100/100.
PERSONAL SKILLS
• Mother tongue: Italian;
• Other language: English;
• Understanding: excellent;
• Writing: excellent;
• Speaking: good.
• Social skills and competences: skill of interaction with other workers understood mainly in academic and athletic environment; good expressive and communicative competences understood mainly in academic enviroment; ability to adapt in different social contexts.
• Organisational skills: during the academic period and my thesis stage, I have obtained organisational competences to face everyday the events in a reaserch lab and to organize the activities of other students.
• Technical skills:
• knowledge of several scientific techniques obtained during the thesis stage in a biomolecular lab: extraction of RNA; extraction of genomic DNA; extraction of proteins; synthesis of cDNA; PCR; electrophoresis of nucleic acid; gel extraction; cloning; ligation; bacterical trasformation; bacterical colture on plates; extraction of plasmidic DNA; digestion using restriction enzymes; antibody precipitation; hybridization in situ; purification of tRNA; preparation of probes; protein dosage;
• ability to use scientific machines (termal cycler, spectrophotometrer, centrifuge, ecc.);
• Computer skills: good knowledge of computer especially good competence of the Office instruments.
• Driving licence: Driving Licence B.
Publications
Research project
DNA METHYLATION ANALYSIS IN ELECTRON MICROSCOPY
Until now, no data are available at electron microscopy on the detection of DNA methylation levels. Consequently, we would use this methodology to highlight possible differences between neoplastic cells and normal control cells. These samples will be analyzed according to their transcriptional rate, either physiological or modified by drugs or active molecules. It will be also interesting verify if chromosome territories in the metaphase nucleus show particular and specific methylation patterns, observable at EM.
PERSONAL INFORMATION
First Name and Surname: Irene Masiello;
Address: Via Maiorino 43, 04023 Formia (LT – Italy);
Telephon: 0771 770364;
Mobile: 328 0639451;
E-mail: irene.masiello@virgilio.it;
Date of Birth: 24/10/1989;
Nationality: Italian.
EDUCATION AND TRAINING
• Title of qualification awarded: Master’s Degree in Biological Sciences (Molecular Diagnostics);
Date: 26/03/2014;
Name and type of organization providing education and training: University “Federico II” of Naples – Faculty of MM. FF. NN. Sciences;
Thesis: Experimental thesis in Molecular Biology – Antibody production for p53 e mdm-like proteins of Mytilus galloprovincialis;
Vote: 110/110 cum laude.
• Title of qualification awarded: Bachelor’s Degree in Biological Sciences;
Date: 23/02/2012;
Name and type of organization providing education and training: University of Naples “Federico II” – Faculty of MM. FF. NN. Sciences;
Thesis: Experimental thesis in Molecular Biology – Danio rerio RPLP0 cloning in an expression vector;
Vote: 110/110 cum laude.
• Title of qualification awarded: High School Qualification in classical studies;
Date: 2008;
Name and type of organization providing education and training: Liceo Classico Vitruvio Pollione – Formia;
Vote: 100/100.
PERSONAL SKILLS
• Mother tongue: Italian;
• Other language: English;
• Understanding: excellent;
• Writing: excellent;
• Speaking: good.
• Social skills and competences: skill of interaction with other workers understood mainly in academic and athletic environment; good expressive and communicative competences understood mainly in academic enviroment; ability to adapt in different social contexts.
• Organisational skills: during the academic period and my thesis stage, I have obtained organisational competences to face everyday the events in a reaserch lab and to organize the activities of other students.
• Technical skills:
• knowledge of several scientific techniques obtained during the thesis stage in a biomolecular lab: extraction of RNA; extraction of genomic DNA; extraction of proteins; synthesis of cDNA; PCR; electrophoresis of nucleic acid; gel extraction; cloning; ligation; bacterical trasformation; bacterical colture on plates; extraction of plasmidic DNA; digestion using restriction enzymes; antibody precipitation; hybridization in situ; purification of tRNA; preparation of probes; protein dosage;
• ability to use scientific machines (termal cycler, spectrophotometrer, centrifuge, ecc.);
• Computer skills: good knowledge of computer especially good competence of the Office instruments.
• Driving licence: Driving Licence B.
Publications
Research project
DNA METHYLATION ANALYSIS IN ELECTRON MICROSCOPY
Until now, no data are available at electron microscopy on the detection of DNA methylation levels. Consequently, we would use this methodology to highlight possible differences between neoplastic cells and normal control cells. These samples will be analyzed according to their transcriptional rate, either physiological or modified by drugs or active molecules. It will be also interesting verify if chromosome territories in the metaphase nucleus show particular and specific methylation patterns, observable at EM.
