Universitą degli studi di Pavia
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Rappa's curriculum
Curriculum
PERSONAL INFORMATIONS:
First name and Surname: Alessandra Rappa
Address: via P.Prestisimone,27
Date and place of birth: 06/08/1986, Palermo(PA) Italy
Phone: 3286984144,
e-mail: alessandra.rappa01@ateneopv.it
ale_rappa @libero.it
EDUCATION AND TRAINING:
1st November 2012 – Present: PhD in Genetics, Molecular and Cellular Biology, University of Pavia, Italy.
Scientific Supervisor: Dr. Patrizia Morbini Dept. of Molecular Medicine - Anatomic Pathology Section – Fondation IRCCS Policlinico “San Matteo”- University of Pavia
October 2010 - July 2012: Master of Science (MS) in Experimental and Applied Biology - Human Biology and Biomedical Sciences, 110/110 with honors, at Dept. of Biology and Biotechnologies “Lazzaro Spallanzani” – University of Pavia.
Title of the thesis: “Expression of a functional TRPV4 channel in endothelial progenitor cells”.
Scientific Supervisor: Prof. Franco Tanzi and Dr. Francesco Moccia, University of Pavia
October 2005 - October 2010: Bechelor's degree, Biological Science, 110/110 with honors, at Azienda Ospedaliera - Ospedali Riuniti “Villa Sofia – Cervello” - University of Palermo.
Title of the thesis: “Laboratory diagnosis in thyroid disease”.
Scinetific Supervisor: Prof. Giuseppe Calvaruso, University of Palermo.
September 2000 - July 2005: High school graduation, 82/100. Liceo Scientifico “Galileo Galilei”, Palermo.
Foreign Languages: English
Mother language: Italian
PERSONAL SKILLS AND COMPETENCES:
DNA/RNA extraction, immunohistochemistry, Polymerase Chain Reaction, Chromogenic and Fluorescence in situ hybridization (ISH-FISH), agarose-gel electrophoresis, hybridization with reverse Dot-Blot technique, sequencing and fragments analysis, cell cultures, calcium imaging, fluorescence microscope, gene silencing with siRNA, ELISA and electrochemiluminescence, clinical biochemistry analysis.
INFORMATIC SKILLS
Good knowledment of Windows Office, and internet browsers, “Origin” program and On-line databases consultation (NCBI- PubMed, Omim, Nucleotide, Ensembl, Blast ecc.)
Research project
Molecular methods applied to the histopathological diagnosis of cancers: from oncogenesis to predictive factors
Mutational analysis of oncogenes implicated in Langerhans Cell Histiocytosis
The purpose of my PhD in Genetics, Molecular and Cellular Biology is to investigate the mutational status of some oncogene in the Histiocytosis.
Histiocytoses encompass a wide range of rare and heterogeneous diseases characterized by the accumulation and proliferation of histiocytes within various tissues. In particular, my study is based on Langerhans Cell Histiocytosis or LCH. The etiology of LCH is unknown. This pathology is a rare neoplasm that occurs predominantly in male children, the incidence of LCH is 2-5 per million children per year in Western Europe.
We have selected a number of cases that arrived to the attention of the Anatomic Pathology Section, University of Pavia. I will study some oncogene implicated in MAPK and PI3K/AKT pathways like BRAF, KRAS, EGFR, c-KIT, PDGFRα, PIK3CA.
The identification of possible mutations in these oncogene could represent a new therapeutic approach for LCH patients. In fact there are inhibitor drugs for this genetic alteration (target therapy).
Publications
Dragoni S, Laforenza U, Bonetti E, Reforgiato M, Poletto V, Lodola F, Bottino C, Guido D, Rappa A, Pareek S, Tomasello M, Guarrera MR, Cinelli MP, Aronica A, Guerra G, Barosi G, Tanzi F, Rosti V, Moccia F.
Enhanced Expression of Stim, Orai, and TRPC Transcripts and Proteins in Endothelial Progenitor Cells Isolated from Patients with Primary Myelofibrosis. PLoS One. 2014 Mar 6;9(3):e91099.
PERSONAL INFORMATIONS:
First name and Surname: Alessandra Rappa
Address: via P.Prestisimone,27
Date and place of birth: 06/08/1986, Palermo(PA) Italy
Phone: 3286984144,
e-mail: alessandra.rappa01@ateneopv.it
ale_rappa @libero.it
EDUCATION AND TRAINING:
1st November 2012 – Present: PhD in Genetics, Molecular and Cellular Biology, University of Pavia, Italy.
Scientific Supervisor: Dr. Patrizia Morbini Dept. of Molecular Medicine - Anatomic Pathology Section – Fondation IRCCS Policlinico “San Matteo”- University of Pavia
October 2010 - July 2012: Master of Science (MS) in Experimental and Applied Biology - Human Biology and Biomedical Sciences, 110/110 with honors, at Dept. of Biology and Biotechnologies “Lazzaro Spallanzani” – University of Pavia.
Title of the thesis: “Expression of a functional TRPV4 channel in endothelial progenitor cells”.
Scientific Supervisor: Prof. Franco Tanzi and Dr. Francesco Moccia, University of Pavia
October 2005 - October 2010: Bechelor's degree, Biological Science, 110/110 with honors, at Azienda Ospedaliera - Ospedali Riuniti “Villa Sofia – Cervello” - University of Palermo.
Title of the thesis: “Laboratory diagnosis in thyroid disease”.
Scinetific Supervisor: Prof. Giuseppe Calvaruso, University of Palermo.
September 2000 - July 2005: High school graduation, 82/100. Liceo Scientifico “Galileo Galilei”, Palermo.
Foreign Languages: English
Mother language: Italian
PERSONAL SKILLS AND COMPETENCES:
DNA/RNA extraction, immunohistochemistry, Polymerase Chain Reaction, Chromogenic and Fluorescence in situ hybridization (ISH-FISH), agarose-gel electrophoresis, hybridization with reverse Dot-Blot technique, sequencing and fragments analysis, cell cultures, calcium imaging, fluorescence microscope, gene silencing with siRNA, ELISA and electrochemiluminescence, clinical biochemistry analysis.
INFORMATIC SKILLS
Good knowledment of Windows Office, and internet browsers, “Origin” program and On-line databases consultation (NCBI- PubMed, Omim, Nucleotide, Ensembl, Blast ecc.)
Research project
Molecular methods applied to the histopathological diagnosis of cancers: from oncogenesis to predictive factors
Mutational analysis of oncogenes implicated in Langerhans Cell Histiocytosis
The purpose of my PhD in Genetics, Molecular and Cellular Biology is to investigate the mutational status of some oncogene in the Histiocytosis.
Histiocytoses encompass a wide range of rare and heterogeneous diseases characterized by the accumulation and proliferation of histiocytes within various tissues. In particular, my study is based on Langerhans Cell Histiocytosis or LCH. The etiology of LCH is unknown. This pathology is a rare neoplasm that occurs predominantly in male children, the incidence of LCH is 2-5 per million children per year in Western Europe.
We have selected a number of cases that arrived to the attention of the Anatomic Pathology Section, University of Pavia. I will study some oncogene implicated in MAPK and PI3K/AKT pathways like BRAF, KRAS, EGFR, c-KIT, PDGFRα, PIK3CA.
The identification of possible mutations in these oncogene could represent a new therapeutic approach for LCH patients. In fact there are inhibitor drugs for this genetic alteration (target therapy).
Publications
Dragoni S, Laforenza U, Bonetti E, Reforgiato M, Poletto V, Lodola F, Bottino C, Guido D, Rappa A, Pareek S, Tomasello M, Guarrera MR, Cinelli MP, Aronica A, Guerra G, Barosi G, Tanzi F, Rosti V, Moccia F.
Enhanced Expression of Stim, Orai, and TRPC Transcripts and Proteins in Endothelial Progenitor Cells Isolated from Patients with Primary Myelofibrosis. PLoS One. 2014 Mar 6;9(3):e91099.
