Universitą degli studi di Pavia
Contenuto della pagina
Bongianino's curriculum
Curriculum
NAME
Rossana Bongianino
DATE AND PLACE OF BIRTH
29/07/1988, Ivrea (TO)
NATIONALITY
Italian
E-MAIL
rossana.bongianino01@universitadipavia.it
EDUCATION
November 2012 – present: PhD student in Genetics, Molecular and Cellular Biology - Universitą degli Studi di Pavia – I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia, Laboratory of Molecular Cardiology.
September 2012 : Master degree in Molecular Biology and Genetics - Universitą degli Studi di Pavia, Faculty of Mathematical, Physical and Natural Sciences. Grade: 110/110 cum laude.
September 2010 : Bachelor's degree in Biotechnology - Universitą degli Studi di Pavia, Faculty of Mathematical, Physical and Natural Sciences, Medicine and Surgery and Pharmacy. Grade: 110/110 cum laude.
July 2007 Scientific High School Diploma : Liceo Scientifico A. Gramsci – Ivrea (TO). Grade: 90/100
PROFESSIONAL EXPERIENCE AND TRAINING
November 2012 - today
Fellowship supported by I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia. Research topic: “Development of viral constructs for the study of new gene therapies to treat calsequestrin deficiencies”. Laboratory of Molecular Cardiology - I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia. Supervisor: Prof. Silvia G. Priori
October 2010 - October 2012
Internship for the Master degree : experimental research in the field of cardiac arrhythmogenic diseases. Laboratory of Molecular Cardiology - I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia. Supervisor: Prof. Silvia G. Priori
September 2009 - September 2010
Internship for the bachelor degree : experimental research applied to the analysis of variability in the mitochondrial genome to study populations and their evolution. Laboratory of Human genetics – Departments of Genetics and Microbiology – Universitą degli Studi di Pavia. Supervisor: Prof. Antonio Torroni.
LANGUAGE
English: good understanding, writing and speaking.
CAPACITY AND TECHNICAL SKILLS
Bio-molecular:
- Cloning techniques.
- Preparation of competent cells and transformation with DNA plasmids, amplification of plasmids in bacterial cultures.
- Purification of DNA plasmids.
- PCR, RT-PCR, Real-Time PCR.
- Analysis of DNA polymorphisms using restriction enzymes (RFLP)
- Extraction of RNA and DNA from tissues and cells.
- Sequencing analysis.
Cellular
- Preparation of mammalian cell cultures.
- Transient transfection of cell cultures.
- Methods of cellular and subcellular fractionation.
- Direct and indirect immunofluorescence techniques.
- Techniques of Optical and Confocal Fluorescence Microscopy.
Biochemistry
- Visible spectrophotometer, UV and IR.
- Electrophoretic techniques (agarose and acrylamide gel)
- Western blot.
- Immunoprecipitation techniques for protein.
Informatic
- Major software in Windows XP, Vista and Windows 7 (Office, Adobe).
- Internet and e-mail management.
- Research in major scientific databases (PubMed, OMIM, Nucleotide, Ensemble, etc..) and use BLAST and FASTA to search for sequence similarity in gene databases.
Publications
Lodola F, Di Pasquale E, Novelli V, Avelino Cruz JE, Denegri M, Leccioli V, Bongianino R, Buoncuore M, Bloise R, Condorelli G, Napolitano C, Priori SG : “CamKII inhibition in human induced pluripotent stem cells derived from Catecholaminergic Polymorphic ventricular tachycardia patients” - 4th Annual Meeting - Foundation Leducq - Transatlantic CamKII Alliace - I.R.C.C.S. Fondazione Salvatore Maugeri, Pavia, 31 August - 1 September / 2012.
Denegri M, Avelino-Cruz JE, De Giusti VC, Lodola L, Curcio A, Bongianino R, Leccioli V, Boncompagni S, Protasi F, Auricchio A, Napolitano C and Priori SG : “Adeno-associated viral gene delivery of calsequestrin 2 protects adult calsequestrin 2-R33Q knock-in mice from developing ventricular tachycardias” American Heart Association Scientific Sessions – Los Angeles CA, 3 - 7 November, 2012
Bongianino R, De Giusti VC, Avelino-Cruz JE, Lodola F, Leccioli V, Denegri M, Tanzi F, Napolitano C, Priori SG : “Functional and molecular characterization of a novel KCNQ1 mutation that causes Long QT Syndrome” – 1st SIRC Workshop on New Roads on Cardiovascular Research, Milano 23 - 24 November 2012
Research project
Gene therapy experimental approaches in cellular and animal models of inherited arrhythmogenic cardiac diseases
My research is part of the studies for the characterization of Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT), an inherited arrhythmic disorder that predisposes to cardiac arrest and sudden death. CPVT occurs in an autosomal dominant form caused by mutations in the gene coding for the cardiac ryanodine receptor (RyR2) and a recessive form caused by mutations in the cardiac calsequestrin (CASQ2). The recessive form of the syndrome is characterized by a decrease in calsequestrin protein expression and consequent alterations of macromolecular complexes involved in calcium homeostasis of the sarcoplasmic reticulum. The aim of my research is to develop viral constructs for gene therapy in transgenic murine models of recessive CPVT in order to obtain the recovery of an healthy phenotype. This work will include cloning and genetic engineering procedures of vectors for the gene transfer of CASQ2 and a later stage of molecular and functional characterization of animal models after infection.
NAME
Rossana Bongianino
DATE AND PLACE OF BIRTH
29/07/1988, Ivrea (TO)
NATIONALITY
Italian
rossana.bongianino01@universitadipavia.it
EDUCATION
November 2012 – present: PhD student in Genetics, Molecular and Cellular Biology - Universitą degli Studi di Pavia – I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia, Laboratory of Molecular Cardiology.
September 2012 : Master degree in Molecular Biology and Genetics - Universitą degli Studi di Pavia, Faculty of Mathematical, Physical and Natural Sciences. Grade: 110/110 cum laude.
September 2010 : Bachelor's degree in Biotechnology - Universitą degli Studi di Pavia, Faculty of Mathematical, Physical and Natural Sciences, Medicine and Surgery and Pharmacy. Grade: 110/110 cum laude.
July 2007 Scientific High School Diploma : Liceo Scientifico A. Gramsci – Ivrea (TO). Grade: 90/100
PROFESSIONAL EXPERIENCE AND TRAINING
November 2012 - today
Fellowship supported by I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia. Research topic: “Development of viral constructs for the study of new gene therapies to treat calsequestrin deficiencies”. Laboratory of Molecular Cardiology - I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia. Supervisor: Prof. Silvia G. Priori
October 2010 - October 2012
Internship for the Master degree : experimental research in the field of cardiac arrhythmogenic diseases. Laboratory of Molecular Cardiology - I.R.C.C.S - Fondazione Salvatore Maugeri – Pavia. Supervisor: Prof. Silvia G. Priori
September 2009 - September 2010
Internship for the bachelor degree : experimental research applied to the analysis of variability in the mitochondrial genome to study populations and their evolution. Laboratory of Human genetics – Departments of Genetics and Microbiology – Universitą degli Studi di Pavia. Supervisor: Prof. Antonio Torroni.
LANGUAGE
English: good understanding, writing and speaking.
CAPACITY AND TECHNICAL SKILLS
Bio-molecular:
- Cloning techniques.
- Preparation of competent cells and transformation with DNA plasmids, amplification of plasmids in bacterial cultures.
- Purification of DNA plasmids.
- PCR, RT-PCR, Real-Time PCR.
- Analysis of DNA polymorphisms using restriction enzymes (RFLP)
- Extraction of RNA and DNA from tissues and cells.
- Sequencing analysis.
Cellular
- Preparation of mammalian cell cultures.
- Transient transfection of cell cultures.
- Methods of cellular and subcellular fractionation.
- Direct and indirect immunofluorescence techniques.
- Techniques of Optical and Confocal Fluorescence Microscopy.
Biochemistry
- Visible spectrophotometer, UV and IR.
- Electrophoretic techniques (agarose and acrylamide gel)
- Western blot.
- Immunoprecipitation techniques for protein.
Informatic
- Major software in Windows XP, Vista and Windows 7 (Office, Adobe).
- Internet and e-mail management.
- Research in major scientific databases (PubMed, OMIM, Nucleotide, Ensemble, etc..) and use BLAST and FASTA to search for sequence similarity in gene databases.
Publications
Lodola F, Di Pasquale E, Novelli V, Avelino Cruz JE, Denegri M, Leccioli V, Bongianino R, Buoncuore M, Bloise R, Condorelli G, Napolitano C, Priori SG : “CamKII inhibition in human induced pluripotent stem cells derived from Catecholaminergic Polymorphic ventricular tachycardia patients” - 4th Annual Meeting - Foundation Leducq - Transatlantic CamKII Alliace - I.R.C.C.S. Fondazione Salvatore Maugeri, Pavia, 31 August - 1 September / 2012.
Denegri M, Avelino-Cruz JE, De Giusti VC, Lodola L, Curcio A, Bongianino R, Leccioli V, Boncompagni S, Protasi F, Auricchio A, Napolitano C and Priori SG : “Adeno-associated viral gene delivery of calsequestrin 2 protects adult calsequestrin 2-R33Q knock-in mice from developing ventricular tachycardias” American Heart Association Scientific Sessions – Los Angeles CA, 3 - 7 November, 2012
Bongianino R, De Giusti VC, Avelino-Cruz JE, Lodola F, Leccioli V, Denegri M, Tanzi F, Napolitano C, Priori SG : “Functional and molecular characterization of a novel KCNQ1 mutation that causes Long QT Syndrome” – 1st SIRC Workshop on New Roads on Cardiovascular Research, Milano 23 - 24 November 2012
Research project
Gene therapy experimental approaches in cellular and animal models of inherited arrhythmogenic cardiac diseases
My research is part of the studies for the characterization of Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT), an inherited arrhythmic disorder that predisposes to cardiac arrest and sudden death. CPVT occurs in an autosomal dominant form caused by mutations in the gene coding for the cardiac ryanodine receptor (RyR2) and a recessive form caused by mutations in the cardiac calsequestrin (CASQ2). The recessive form of the syndrome is characterized by a decrease in calsequestrin protein expression and consequent alterations of macromolecular complexes involved in calcium homeostasis of the sarcoplasmic reticulum. The aim of my research is to develop viral constructs for gene therapy in transgenic murine models of recessive CPVT in order to obtain the recovery of an healthy phenotype. This work will include cloning and genetic engineering procedures of vectors for the gene transfer of CASQ2 and a later stage of molecular and functional characterization of animal models after infection.
