Universitą degli studi di Pavia
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Travaglino's cv
CURRICULUM
PERSONAL INFORMATIONS
Name: Stefano
Surname: Travaglino
Address: Via Robecchi 26, 27020 Gravellona Lomellina (PV), Italy
Phone number: 333 4282899
E-mail: stefano.travaglino01@universitadipavia.it, stetrava88@gmail.com
Nationality: Italian
Date of birth: 09/07/1988
EDUCATION
11/2012 - Present
PhD in Genetics, Molecular and Cellular Biology.
“Camillo Golgi” Doctoral School in Life Sciences, University of Pavia, Italy.
Scientific supervisor: Prof. Edda De Rossi - Laboratory of General Microbiology. Department of Biology and Biotechnology “L. Spallanzani”.
10/2010 - 07/2012
Master degree in Molecular Biology and Genetics at University of Pavia. Final grade: 110/110 cum laude. Laboratory of General Microbiology (Prinicipal investigator: Prof. Edda De Rossi - Department of Biology and Biotechnology “L. Spallanzani”). Thesis title: “Expression of the mycobacterial membrane protein MmpL3: a preliminary study”.
10/2007 - 07/2010
Bachelor degree in Biotechnology at University of Pavia. Final grade: 110/110 cum laude. Laboratory of Clinical Microbiology (Principal investigator: Prof. Laura Pagani - Department of Morphological, Eidological and Clinical Sciences). Thesis title: “Diffusion of KPC carbapenemase-producing Klebsiella pneumoniae strains in italian hospitals”.
10/2002 - 07/2007
Scientific maturity diploma at “A. Omodeo” High School (Mortara, PV, Italy).
Final grade: 100/100.
WORKING EXPERIENCE
11/2012 – Present
Research fellowship - PhD in Genetics, Molecular and Cellular Biology.
Scientific supervisor: Prof. Edda De Rossi - Laboratory of General Microbiology. Department of Biology and Biotechnology “L. Spallanzani”.
Laboratory activity: characterization of the cellular target of antitubercular compounds.
LANGUAGES
Italian. Native language.
English. Reading skill: excellent. Writing skill: excellent. Speaking skill: good.
TECHNICAL SKILLS
Microbiology, Genetics, Molecular Biology and Biochemistry basic techniques.
Manipulation and transformation of Escherichia coli, Mycobacterium smegmatis and M. bovis BCG cells, isolation of mutants resistant to antitubercular compounds, MIC determination in solid and liquid media, Gram staining, API test, Kirby-Bauer test, analysis of clonal relationships between bacterial strains using Pulse-Fiel Gel Electrophoresis. Gene cloning, preparation and purification of plasmid and genomic DNA , agarose gel electrophoresis, PCR, Real Time-PCR. Protein expression techniques, affinity chromatography, SDS-PAGE, isoelectrofocusing of proteic extracts. Analysis of nucleotide and amino acid sequences.
INFORMATIC SKILLS
Excellent knowledge of the Windows operative system.
Good knowledge of the Office suite (Word/Excel/PowerPoint/Publisher).
Basic knowledge of graphical editing programs (PhotoShop).
Scientific bibliographic search tools (NCBI-PubMed and EB-Eye)
Bioinformatic tools (BLAST, LALIGN, Chromas, Primer3, EXPASY proteomics server, NCBI and EMBL-EBI databases).
RESEARCH ACTIVITY
Elucidating the organization of the mycobacterial mmpL3 locus
Tuberculosis (TB) is one of the most widespread diseases in the world. Despite its incidence is slowly decreasing thanks to effective control strategies, new challenges are emerging. There is concern about the emergence and diffusion of increasingly drug resistant Mycobacterium tuberculosis strains. In this perspective, it is urgent the need of new and better therapeutic tools. In particular, new drugs are needed not only to fight resistant strains, but also to improve the therapeutic regimens which are very expensive and time-consuming, and demand a lot of effort both from the patients and the caregivers. When developing new drugs, it is fundamental the validation and characterization at molecular level of their target.
Recently, the compound with antitubercular activity BM212 and its derivatives have been found to target MmpL3. This mycobacterial membrane transporter belongs to the Mycobacterial Membrane Protein, Large (MmpLs) family and is involved in the essential mycolic acid biosynthetic pathway, but is still poorly characterized.
My research activity entails the study, in different mycobacterial species, of the mmpL3 gene and its adjacent region; possibly, the study will be extended also to other mmpL genes, with the aim to better define the role of this family peculiar to the genus Mycobacterium and few other related genera.
PUBLICATIONS
Battaglia S, Ortiz Canseco J, Travaglino S, La Rosa V, De Rossi E. Characterizing the MmpL3 protein, a novel target for new antituberculars. XII FISV Congress. 24th-27th September 2012, Rome.
PERSONAL INFORMATIONS
Name: Stefano
Surname: Travaglino
Address: Via Robecchi 26, 27020 Gravellona Lomellina (PV), Italy
Phone number: 333 4282899
E-mail: stefano.travaglino01@universitadipavia.it, stetrava88@gmail.com
Nationality: Italian
Date of birth: 09/07/1988
EDUCATION
11/2012 - Present
PhD in Genetics, Molecular and Cellular Biology.
“Camillo Golgi” Doctoral School in Life Sciences, University of Pavia, Italy.
Scientific supervisor: Prof. Edda De Rossi - Laboratory of General Microbiology. Department of Biology and Biotechnology “L. Spallanzani”.
10/2010 - 07/2012
Master degree in Molecular Biology and Genetics at University of Pavia. Final grade: 110/110 cum laude. Laboratory of General Microbiology (Prinicipal investigator: Prof. Edda De Rossi - Department of Biology and Biotechnology “L. Spallanzani”). Thesis title: “Expression of the mycobacterial membrane protein MmpL3: a preliminary study”.
10/2007 - 07/2010
Bachelor degree in Biotechnology at University of Pavia. Final grade: 110/110 cum laude. Laboratory of Clinical Microbiology (Principal investigator: Prof. Laura Pagani - Department of Morphological, Eidological and Clinical Sciences). Thesis title: “Diffusion of KPC carbapenemase-producing Klebsiella pneumoniae strains in italian hospitals”.
10/2002 - 07/2007
Scientific maturity diploma at “A. Omodeo” High School (Mortara, PV, Italy).
Final grade: 100/100.
WORKING EXPERIENCE
11/2012 – Present
Research fellowship - PhD in Genetics, Molecular and Cellular Biology.
Scientific supervisor: Prof. Edda De Rossi - Laboratory of General Microbiology. Department of Biology and Biotechnology “L. Spallanzani”.
Laboratory activity: characterization of the cellular target of antitubercular compounds.
LANGUAGES
Italian. Native language.
English. Reading skill: excellent. Writing skill: excellent. Speaking skill: good.
TECHNICAL SKILLS
Microbiology, Genetics, Molecular Biology and Biochemistry basic techniques.
Manipulation and transformation of Escherichia coli, Mycobacterium smegmatis and M. bovis BCG cells, isolation of mutants resistant to antitubercular compounds, MIC determination in solid and liquid media, Gram staining, API test, Kirby-Bauer test, analysis of clonal relationships between bacterial strains using Pulse-Fiel Gel Electrophoresis. Gene cloning, preparation and purification of plasmid and genomic DNA , agarose gel electrophoresis, PCR, Real Time-PCR. Protein expression techniques, affinity chromatography, SDS-PAGE, isoelectrofocusing of proteic extracts. Analysis of nucleotide and amino acid sequences.
INFORMATIC SKILLS
Excellent knowledge of the Windows operative system.
Good knowledge of the Office suite (Word/Excel/PowerPoint/Publisher).
Basic knowledge of graphical editing programs (PhotoShop).
Scientific bibliographic search tools (NCBI-PubMed and EB-Eye)
Bioinformatic tools (BLAST, LALIGN, Chromas, Primer3, EXPASY proteomics server, NCBI and EMBL-EBI databases).
RESEARCH ACTIVITY
Elucidating the organization of the mycobacterial mmpL3 locus
Tuberculosis (TB) is one of the most widespread diseases in the world. Despite its incidence is slowly decreasing thanks to effective control strategies, new challenges are emerging. There is concern about the emergence and diffusion of increasingly drug resistant Mycobacterium tuberculosis strains. In this perspective, it is urgent the need of new and better therapeutic tools. In particular, new drugs are needed not only to fight resistant strains, but also to improve the therapeutic regimens which are very expensive and time-consuming, and demand a lot of effort both from the patients and the caregivers. When developing new drugs, it is fundamental the validation and characterization at molecular level of their target.
Recently, the compound with antitubercular activity BM212 and its derivatives have been found to target MmpL3. This mycobacterial membrane transporter belongs to the Mycobacterial Membrane Protein, Large (MmpLs) family and is involved in the essential mycolic acid biosynthetic pathway, but is still poorly characterized.
My research activity entails the study, in different mycobacterial species, of the mmpL3 gene and its adjacent region; possibly, the study will be extended also to other mmpL genes, with the aim to better define the role of this family peculiar to the genus Mycobacterium and few other related genera.
PUBLICATIONS
Battaglia S, Ortiz Canseco J, Travaglino S, La Rosa V, De Rossi E. Characterizing the MmpL3 protein, a novel target for new antituberculars. XII FISV Congress. 24th-27th September 2012, Rome.
