Universitą degli studi di Pavia


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Milani's CV

Name: Milani, Pamela
Address: 6/i, Matteotti st., 23807, Merate, LECCO, Italy
Telephone: +393397496706
E-mail: pame.milani@gmail.com
Nationality: Italian

Education and training
From November 2009: PhD program at the University of Pavia
From March 2008 to July 2009: Master’s degree in Biology at the University of Pavia; Molecular Biology curriculum (final vote: 110/110 cum laude)
From 0ctober 2004 to February 2008: Bachelor’s degree in Biological Sciences at the University of Milano-Bicocca (UNIMIB); Biomolecular curriculum (Final vote: 110/110 cum laude)
From 2000 to 2004: Secondary schools, Scientific lyceum (Final vote 100/100)

Technical skills and competences
-Extraction of lymphocyte from peripheral blood
- Cultivation of various cell lines
-Cloning technique and production of vectors for cell transfectin
-Cell Transfection using Lipofectamine and Electroporation
-Cell treatment with different agents (i.e. hydrogen peroxide, tBHQ ...)
-Extraction of DNA and RNA from peripheral blood cells and various cell lines
-Polymerase chain raction (PCR)
- Quantitative analysis by real time PCR (qPCR), TaqMan and SybrGreen
- Preparation and analysis of agarose and poliacrilamide gel
- Sequencing of the DNA using the instrument ABI 310 Genetic Analyzer
-Protein extraction and quantification by BCA and Bradford
-Extraction and analysis of cytoplasmatic, nuclear and chromatin-associated proteins
- Immunofluorescence on primary cells (lymphocytes) and neuronal cell lines
-Electrophoretic Mobility Shift Assay (EMSA) using infrared probes
-Chromatin Immunoprecipitation and qPCR (ChIP-qPCR)
- RNA immunoprecipitation and qPCR (RIP-qPCR)
- Identification and quantification of protein by Western blotting
- Identification and quantification of protein by In Cell Western Blot (Odyssey)
-Immunoprecipitation and co-immunoprecipitation
-Detection and analysis of protein oxidation
-Cytofluorimetric analysis for Reactive Oxygen Species (ROS) and protein expression
-Statistical analysis, GraphPad Prism programs

Other languages: English (Reading skills: good; Writing skills: good; Verbal skills: good)

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