Universitą degli studi di Pavia
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Bono's cv
Curriculum
PERSONAL INFORMATION:
Name: BARTOLO BONO
Nationality: Italian
Home address: 275, Via Umberto I°, 91021 Campobello di Mazara (TP), Italy
Date and place of birth: Castelvetrano, 25/11/1982
Telephone: +39.3206003231
E-mail: bono@igm.cnr.it; bonobartolo@libero.it
EDUCATION and TRAINING:
• 10/2009 - 04/2013 Master Degree in “Cellular and Molecular Biology” - Biomedical address
University of Turin, Italy
grade: 103/110
• 10/2001 - 07/2009 Bachelor Degree in “Biological Sciences” - Biosanitary address
University of Palermo, Italy
grade: 108/110
• 09/1996 - 07/2001 Scientific High School Degree
Liceo Scientifico “m. Cipolla”, Italy
grade: 100/100
WORK and PROFESSIONAL EXPERIENCE:
• 11/2013 - Present: Ph.D in Genetics, Molecular and Cellular Biology, University of Pavia;
Project: “Tumor heterogeneity analysis using in vitro transformed human cells”;
Genomics and Human Genetics Laboratory; Institute of Molecular Genetics - Italian National Research Council (IGM-CNR), Pavia, Italy;
Scientific Supervisor: Dott. Chiara Mondello;
Scholarship I.N.P.S. associated with Ph.D
• 04/2013 - 10/2013: Collaboration as Laboratory Assistant;
- “Molecular diagnostics in the field of Cancer Genetics”;
- “ Identification of alternative transcripts in G396D-associated MAP patients”;
Anatomic Pathology Division, Genetics and Molecular Pathology Laboratory, Institute for Cancer Research and Treatment (IRCC), Candiolo, Turin, Italy;
Dr. Tiziana Venesio,
• 03/2011 - 04/2013: Internship for the Master Degree;
Project: “Characterization of alternative aberrant transcripts G396D-associated germline mutation in MAP patients”;
Anatomic Pathology Division, Genetics and Molecular Pathology Laboratory, Institute for Cancer Research and Treatment (IRCC), Candiolo, Turin, Italy;
Scientific Supervisors: Dott. Tiziana Venesio, Prof. Michele De Bortoli;
• 09/2007 - 06/2009: Intership for the Bachelor Degree;
Project: “Techniques for the study of the responses induced by stimulation of enteric neurons in gastrointestinal smooth muscles”;
General Physiology Laboratory, STEBICEF, Department of Cellular and Development Biology “A. Monroy”, University of Palermo, Italy.
Scientific Supervisors: Prof. Rosa Serio; Dott. Maria Grazia Zizzo;
ADDITIONAL CERTIFICATES and LICENSES:
• 10/12/2013: Qualification to exercise the Profession of Biologist
ORGANISATIONAL and SOCIAL SKILLS
Good organisational skills and communication, determination, self-motivated. I have worked in different scientific research teams during my university studies, collaborating with my colleagues and people from other groups; I optimized my organization sense and my competences; I can work in stressful conditions and respect deadlines.
I also developed an excellent adaptation in no-profit cultural and multicultural environments. Education, kindness and respect are my priority.
TECHNICAL SKILLS:
• MOLECULAR BIOLOGY and BIOCHEMISTRY: nucleic acids purification and quantification, PCR, Nested PCR, RT-PCR, qPCR, qRT-PCR, agarose gel electrophoresis, direct sequencing (Sanger method), pyrosequencing; protein extraction and quantification, SDS-PAGE, western blot.
• CELLULAR BIOLOGY: eukaryotic cell culture, optical microscopy.
• PHYSIOLOGY: microelectrode recording techniques, isolated organ bath system.
• BIOINFORMATICS: analysis of electropherograms and pyrograms (BioEdit, PyroMark); using of scientific software and web tools (Quantity One, Primer3, AnnHyb, Blastn, Blastp, ClustalW, NEBcutter, ESEFinder, SpliceAid, Human Splicing Finder); analysis of genomic sequences (NCBI, Ensembl).
• BIOSTATISTICS: Excel, R and SPSS software
LINGUSTIC COMPETENCES:
• Italian: mother tongue
• English: good level (understanding, speaking and writing)
• French: basic level
• Spanish: basic level
INFORMATIC SKILLS:
Competent with most Microsoft Office (Word, Power Point, Excel) and Windows (XP,Vista,7) tools.
ATTENDANCE TO MEETINGS AND COURSES:
• 4th Annual Meeting, SysBioM (Complex Systems in Molecular Biology and Medicine) interdisciplinary research cluster (Turin ,Italy - February 5, 2010);
• A.I.F.E.G. Annual Meeting, IRCCS Foundation - National Cancer Institute (Milan, Italy - June 27,2011);
• QIAGEN Event: “Personalized medicine: the Pathologist at the forefront” (Edo and Elvo Tempia Foundation, Biella, Italy - June 26, 2013);
• XI° A.I.F.E.G. National Meeting, Collegio Ghislieri, (Pavia, Italy - October 24-25, 2013)
Research Project
Tumor heterogeneity analysis using in vitro transformed human cells
My research project concerns the analysis of cellular heterogeneity in tumors. I will perform this study using a particular cellular line that, in particular conditions, it mimics the neoplastic transformed status of human fibroblasts; I’m going to investigate the presence of cells with cancer stemness features and eventually their role in tumorigenesis.
Publications
Abstracts
• Bartolo Bono, Antonella Balsamo, Elisa Giacomin, Alessandra Viel and Tiziana Venesio, “Characterization of alternative transcripts in MUTYH-associated-polyposis patients carrying the p.Gly396Asp mutation”. XI Congresso Nazionale A.I.F.E.G., 24-25/10/2013, Pavia. Oral presentation.
• Mondello C, Chiodi I, Bono B, Peritore M, Monticone G, Pizzinga M, Frapolli R and D’Incalci M. Deregulation of c-myc and GNL3 in tumorspheres from in vitro transformed cell lines. 4th World Congress on Cell Science & Stem Cell Research, Valencia, June 24-26, 2014. J Cell Sci Ther, 5:4, 2014.
PERSONAL INFORMATION:
Name: BARTOLO BONO
Nationality: Italian
Home address: 275, Via Umberto I°, 91021 Campobello di Mazara (TP), Italy
Date and place of birth: Castelvetrano, 25/11/1982
Telephone: +39.3206003231
E-mail: bono@igm.cnr.it; bonobartolo@libero.it
EDUCATION and TRAINING:
• 10/2009 - 04/2013 Master Degree in “Cellular and Molecular Biology” - Biomedical address
University of Turin, Italy
grade: 103/110
• 10/2001 - 07/2009 Bachelor Degree in “Biological Sciences” - Biosanitary address
University of Palermo, Italy
grade: 108/110
• 09/1996 - 07/2001 Scientific High School Degree
Liceo Scientifico “m. Cipolla”, Italy
grade: 100/100
WORK and PROFESSIONAL EXPERIENCE:
• 11/2013 - Present: Ph.D in Genetics, Molecular and Cellular Biology, University of Pavia;
Project: “Tumor heterogeneity analysis using in vitro transformed human cells”;
Genomics and Human Genetics Laboratory; Institute of Molecular Genetics - Italian National Research Council (IGM-CNR), Pavia, Italy;
Scientific Supervisor: Dott. Chiara Mondello;
Scholarship I.N.P.S. associated with Ph.D
• 04/2013 - 10/2013: Collaboration as Laboratory Assistant;
- “Molecular diagnostics in the field of Cancer Genetics”;
- “ Identification of alternative transcripts in G396D-associated MAP patients”;
Anatomic Pathology Division, Genetics and Molecular Pathology Laboratory, Institute for Cancer Research and Treatment (IRCC), Candiolo, Turin, Italy;
Dr. Tiziana Venesio,
• 03/2011 - 04/2013: Internship for the Master Degree;
Project: “Characterization of alternative aberrant transcripts G396D-associated germline mutation in MAP patients”;
Anatomic Pathology Division, Genetics and Molecular Pathology Laboratory, Institute for Cancer Research and Treatment (IRCC), Candiolo, Turin, Italy;
Scientific Supervisors: Dott. Tiziana Venesio, Prof. Michele De Bortoli;
• 09/2007 - 06/2009: Intership for the Bachelor Degree;
Project: “Techniques for the study of the responses induced by stimulation of enteric neurons in gastrointestinal smooth muscles”;
General Physiology Laboratory, STEBICEF, Department of Cellular and Development Biology “A. Monroy”, University of Palermo, Italy.
Scientific Supervisors: Prof. Rosa Serio; Dott. Maria Grazia Zizzo;
ADDITIONAL CERTIFICATES and LICENSES:
• 10/12/2013: Qualification to exercise the Profession of Biologist
ORGANISATIONAL and SOCIAL SKILLS
Good organisational skills and communication, determination, self-motivated. I have worked in different scientific research teams during my university studies, collaborating with my colleagues and people from other groups; I optimized my organization sense and my competences; I can work in stressful conditions and respect deadlines.
I also developed an excellent adaptation in no-profit cultural and multicultural environments. Education, kindness and respect are my priority.
TECHNICAL SKILLS:
• MOLECULAR BIOLOGY and BIOCHEMISTRY: nucleic acids purification and quantification, PCR, Nested PCR, RT-PCR, qPCR, qRT-PCR, agarose gel electrophoresis, direct sequencing (Sanger method), pyrosequencing; protein extraction and quantification, SDS-PAGE, western blot.
• CELLULAR BIOLOGY: eukaryotic cell culture, optical microscopy.
• PHYSIOLOGY: microelectrode recording techniques, isolated organ bath system.
• BIOINFORMATICS: analysis of electropherograms and pyrograms (BioEdit, PyroMark); using of scientific software and web tools (Quantity One, Primer3, AnnHyb, Blastn, Blastp, ClustalW, NEBcutter, ESEFinder, SpliceAid, Human Splicing Finder); analysis of genomic sequences (NCBI, Ensembl).
• BIOSTATISTICS: Excel, R and SPSS software
LINGUSTIC COMPETENCES:
• Italian: mother tongue
• English: good level (understanding, speaking and writing)
• French: basic level
• Spanish: basic level
INFORMATIC SKILLS:
Competent with most Microsoft Office (Word, Power Point, Excel) and Windows (XP,Vista,7) tools.
ATTENDANCE TO MEETINGS AND COURSES:
• 4th Annual Meeting, SysBioM (Complex Systems in Molecular Biology and Medicine) interdisciplinary research cluster (Turin ,Italy - February 5, 2010);
• A.I.F.E.G. Annual Meeting, IRCCS Foundation - National Cancer Institute (Milan, Italy - June 27,2011);
• QIAGEN Event: “Personalized medicine: the Pathologist at the forefront” (Edo and Elvo Tempia Foundation, Biella, Italy - June 26, 2013);
• XI° A.I.F.E.G. National Meeting, Collegio Ghislieri, (Pavia, Italy - October 24-25, 2013)
Research Project
Tumor heterogeneity analysis using in vitro transformed human cells
My research project concerns the analysis of cellular heterogeneity in tumors. I will perform this study using a particular cellular line that, in particular conditions, it mimics the neoplastic transformed status of human fibroblasts; I’m going to investigate the presence of cells with cancer stemness features and eventually their role in tumorigenesis.
Publications
Abstracts
• Bartolo Bono, Antonella Balsamo, Elisa Giacomin, Alessandra Viel and Tiziana Venesio, “Characterization of alternative transcripts in MUTYH-associated-polyposis patients carrying the p.Gly396Asp mutation”. XI Congresso Nazionale A.I.F.E.G., 24-25/10/2013, Pavia. Oral presentation.
• Mondello C, Chiodi I, Bono B, Peritore M, Monticone G, Pizzinga M, Frapolli R and D’Incalci M. Deregulation of c-myc and GNL3 in tumorspheres from in vitro transformed cell lines. 4th World Congress on Cell Science & Stem Cell Research, Valencia, June 24-26, 2014. J Cell Sci Ther, 5:4, 2014.
