Universitą degli studi di Pavia
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Santonastaso's cv
Curriculum
PERSONAL INFORMATION
First name and surname: Alice Santonastaso
Date of birth: 16th of March 1989
Birthplace: Novi Ligure (AL)
Nationality: Italian
Address: via Verdi, 84 – 15067, Novi Ligure (AL)
E-mail: alice.santonastaso01@ateneopv.it
Mobile: +39 3468298013
EDUCATION AND TRAINING
- November 2013 to current date
PhD in Genetics, Molecular and Cellular Biology, XXIX cycle (2013/2016)
University of Pavia, Department of Molecular Medicine, Unit of Immunology and General Pathology
Supervisor: Dr. Claudia Scotti
- October 2011 - July 2013
Master Degree in Medical and Pharmaceutical Biotechnology (110/110 with honours)
University of Pavia
Title of the experimental thesis: "Purification and Crystallization of NQ11/7.22.17 Fab"
Supervisor: Dr. Claudia Scotti
- October 2008 – September 2011
Degree in Biotechnology (110/110 with honours)
University of Pavia
Title of the experimental thesis: “Evaluation of circulating Tie+ monocytes in patients with Ph-negative myeloproliferative diseases"
Supervisor: Dr. Daniela Montagna
- September 2003 - July 2008
High School Diploma (Classical studies) (100/100)
Liceo Classico “A. Doria”, Novi Ligure (AL)
WORK EXPERIENCE
- November 2013 to current date
PhD in Genetics, Molecular and Cellular Biology, XXIX cycle (2013/2016)
Project: Generation and engineering of anti-Lipoprotein(a) antibodies for diagnostic and therapeutic purposes.
Department of Molecular Medicine, Unit of Immunology and General Pathology
University of Pavia
Supervisor: Dr. Claudia Scotti
- September 2012 - July 2013
Internship for the Master Degree in Medical and Pharmaceutical Biotechnology
Subjects of internship: production, purification and crystallization of antibody fragments derived from mouse monoclonal antibodies.
Department of Molecular Medicine, Unit of Immunology and General Pathology
University of Pavia
Supervisor: Dr. Claudia Scotti
- October 2010 - September 2011
Internship for the Degree in Biotechnology
Subject of internship: quanlitative and quantitative evaluation of monocyte populations in blood and spleen samples from patients affected with myeloproliferative diseases.
Department of Pediatric Sciences, Laboratories of Oncohaematology and Bone Marrow Transplantation
Fondazione I.R.C.C.S Policlinico San Matteo, Pavia
Supervisor: Dr. Daniela Montagna
ADDITIONAL EDUCATIONAL AND RESEARCH ACTIVITIES
- November 2012 - May 2013
Partecipation to the "Journal Club" initiative: a series of seminaries in English language focused on recent and relevant scientific studies. Department of Molecular Medicine, University of Pavia.
LABORATORY COMPETENCES
- Cell culture techniques
- DNA and RNA extraction and purification
- Molecular biology techniques (PCR, retrotranscription, gene cloning, bacterial transformation through heat-shock, enzymatic digestion, ligation, expression and purification of recombinant proteins)
- Immunocytochemistry
- Nucleic acid and protein analysis and separation techniques (gel electrophoresis, Western blot, chromatography)
- Microscopy
- Crystallography (sitting-drop technique)
- Flow cytometry
COMPUTER COMPETENCES
- Windows and Microsoft Office tools (Word, Excel, Access, PowerPoint)
- Adobe Photoshop (basic level)
- Biological Databases (NCBI, PDB, IMGT, GenBank,..)
- Softwares for the analysis of genetic sequences, primer design (ClustalW, BioEdit)
- Software of molecular modelling (PyMol)
- Software "R" (basic level)
2008 – ECDL Certificate, European Computer Driving Licence.
LINGUISTIC COMPETENCES
- Italian language: mother tongue
- English language: upper-intermediate level
- French language: basic level
2006 – PET Certificate, Preliminary Engligh Test (with merit)
2005 – Study holiday in Portsmouth, UK.
Research Project
Generation and Engineering of anti-Lp(a) antibodies for diagnostic and therapeutic purposes
Lipoprotein(a) [Lp(a)] is a novel cardiovascular risk factor. The project will focus on the generation of anti-Lp(a) antibodies for diagnostic and therapeutic purposes. After immunization and hybridoma generation, the genes encoding the VH and VL domains of the resulting antibodies will be amplified and sequenced. After cloning in scFv and Fab format, they will be overexpressed, purified and used as building blocks for systems aimed at the diagnosis of hyperlipoproteinemia. Their potential role as drugs will also be explored in in vitro systems.
Publications
PERSONAL INFORMATION
First name and surname: Alice Santonastaso
Date of birth: 16th of March 1989
Birthplace: Novi Ligure (AL)
Nationality: Italian
Address: via Verdi, 84 – 15067, Novi Ligure (AL)
E-mail: alice.santonastaso01@ateneopv.it
Mobile: +39 3468298013
EDUCATION AND TRAINING
- November 2013 to current date
PhD in Genetics, Molecular and Cellular Biology, XXIX cycle (2013/2016)
University of Pavia, Department of Molecular Medicine, Unit of Immunology and General Pathology
Supervisor: Dr. Claudia Scotti
- October 2011 - July 2013
Master Degree in Medical and Pharmaceutical Biotechnology (110/110 with honours)
University of Pavia
Title of the experimental thesis: "Purification and Crystallization of NQ11/7.22.17 Fab"
Supervisor: Dr. Claudia Scotti
- October 2008 – September 2011
Degree in Biotechnology (110/110 with honours)
University of Pavia
Title of the experimental thesis: “Evaluation of circulating Tie+ monocytes in patients with Ph-negative myeloproliferative diseases"
Supervisor: Dr. Daniela Montagna
- September 2003 - July 2008
High School Diploma (Classical studies) (100/100)
Liceo Classico “A. Doria”, Novi Ligure (AL)
WORK EXPERIENCE
- November 2013 to current date
PhD in Genetics, Molecular and Cellular Biology, XXIX cycle (2013/2016)
Project: Generation and engineering of anti-Lipoprotein(a) antibodies for diagnostic and therapeutic purposes.
Department of Molecular Medicine, Unit of Immunology and General Pathology
University of Pavia
Supervisor: Dr. Claudia Scotti
- September 2012 - July 2013
Internship for the Master Degree in Medical and Pharmaceutical Biotechnology
Subjects of internship: production, purification and crystallization of antibody fragments derived from mouse monoclonal antibodies.
Department of Molecular Medicine, Unit of Immunology and General Pathology
University of Pavia
Supervisor: Dr. Claudia Scotti
- October 2010 - September 2011
Internship for the Degree in Biotechnology
Subject of internship: quanlitative and quantitative evaluation of monocyte populations in blood and spleen samples from patients affected with myeloproliferative diseases.
Department of Pediatric Sciences, Laboratories of Oncohaematology and Bone Marrow Transplantation
Fondazione I.R.C.C.S Policlinico San Matteo, Pavia
Supervisor: Dr. Daniela Montagna
ADDITIONAL EDUCATIONAL AND RESEARCH ACTIVITIES
- November 2012 - May 2013
Partecipation to the "Journal Club" initiative: a series of seminaries in English language focused on recent and relevant scientific studies. Department of Molecular Medicine, University of Pavia.
LABORATORY COMPETENCES
- Cell culture techniques
- DNA and RNA extraction and purification
- Molecular biology techniques (PCR, retrotranscription, gene cloning, bacterial transformation through heat-shock, enzymatic digestion, ligation, expression and purification of recombinant proteins)
- Immunocytochemistry
- Nucleic acid and protein analysis and separation techniques (gel electrophoresis, Western blot, chromatography)
- Microscopy
- Crystallography (sitting-drop technique)
- Flow cytometry
COMPUTER COMPETENCES
- Windows and Microsoft Office tools (Word, Excel, Access, PowerPoint)
- Adobe Photoshop (basic level)
- Biological Databases (NCBI, PDB, IMGT, GenBank,..)
- Softwares for the analysis of genetic sequences, primer design (ClustalW, BioEdit)
- Software of molecular modelling (PyMol)
- Software "R" (basic level)
2008 – ECDL Certificate, European Computer Driving Licence.
LINGUISTIC COMPETENCES
- Italian language: mother tongue
- English language: upper-intermediate level
- French language: basic level
2006 – PET Certificate, Preliminary Engligh Test (with merit)
2005 – Study holiday in Portsmouth, UK.
Research Project
Generation and Engineering of anti-Lp(a) antibodies for diagnostic and therapeutic purposes
Lipoprotein(a) [Lp(a)] is a novel cardiovascular risk factor. The project will focus on the generation of anti-Lp(a) antibodies for diagnostic and therapeutic purposes. After immunization and hybridoma generation, the genes encoding the VH and VL domains of the resulting antibodies will be amplified and sequenced. After cloning in scFv and Fab format, they will be overexpressed, purified and used as building blocks for systems aimed at the diagnosis of hyperlipoproteinemia. Their potential role as drugs will also be explored in in vitro systems.
Publications
