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Mustafa's cv
Curriculum
Name: Noor Hussein Mustafa
Nationality: Jordanian
Date & Place of Birth: June/02/1984. Kuwait.
Marital status: married
Nooroo20038@hotmail.com
Education
M.Sc. in Biological Sciences
The Hashemite University, Department of Biological Sciences and Biotechnology, Zarqa, Jordan January 2009 with an average of 3.65 Out of 4.00 rating: Excellent .
Thesis title: “The Effect of Supraphysiological Dosing of Anabolic –Androgenic Steroids on Chromatin and Expression of Androgen Receptor of the Rat Testis –Supporting Cells”
Supervisor: Dr. Lubna Tahtamouni, Co-Supervisor: Dr. Maher Abdalla
B.Sc. in Biological Sciences
Hashemite University, Department of Biological Sciences and Biotechnology, Zarqa, Jordan, June 2006 with an average of 3.73 Out of 4.00
rating: Excellent. get number 2nd In emamination Efficiency ofuniversity on all undergraduates Jordanian universities
Scientific Stream
Scientific Stream, Muscat, Oman, July 2002 with percentage average 96.51%
Professional Experience
Lecturer * King Saud bin Abdul Aziz for health science-UPPP September 2011 to September 2013
Lecturer * Department of Biological Sciences and Biotechnology, The Hashemite University February 2011 to June 2011
Lecturer * King Saud University, Kingdom of Saudi Arabia-ALKJARJ October 2009 to September 2010
Research Assistant Lab. Tissue culture, Department of Radiography, The Hashemite University, Zarqa, Jordan. December 2008 to October
2009
Teaching Assistant* Department of Biology, The Hashemite University, Zarqa, Jordan February 2009 to October
Student Teaching Assistant * Department of Biology, The Hashemite University, Zarqa, Jordan. September 2006 to June 2008 (four semester)
Research Assistant Tissue culture, immunology and Microtechniques lab. Department of Biology, The Hashemite University, Zarqa, Jordan.
September 2006 to June 2008
Research Assistant (when I was undergraduate I worked as volunteer) Neuroscience lab. Department of Biology, The Hashemite University, Zarqa, Jordan February 2006 to October 2006
*Teaching Experience
2006 to present – Immunology, Tissue Culture Techniques, Developmental Biology, Applied Developmental Biology, Gene Expression,
Microbiology, Recombenant DNA Techniques, Microtechniques, Hematolgy, Biochimistry , Chordates ,Physiology , Cell Biology , General Practical Biology (1); and General Practical Biology (2)
Training course
(video education training tutorials) http://www.knowledgecity.com
October 2011 to May 2012
1-The Art of Team Building
2- Problem Solving & Decision Making
3- The Art of Motivation
4- Time Management
5- Strategic Thinking
6- The Art of Negotiation
7- Effective Communication
8- Gender Communication
9- Generational Communication
10- Group Communication
11- Managing and Supervising
12- Elements of Leadership and Supervision
13- Intercultural Communication
14- Interpersonal Communication National guard health affairs
Medical terminology
National Guard Health Affairs (NGHA)\languish training February 2012 to May 2012
1-Methodolgy of sperm retrival in non obstructive azoospermia
2-Oocyte and ovarian tissue freezing
The Farah hospital on the 8th of April 2009 on the 8th of April 2009
Immunohistochemistry
The Higher Council For Science And Technology In Association With The Jordan Company For Antibody Production (MONOJO). October 2008 (24 hours)
Principles and Practices of Assisted Reproduction: In vitro fertilization (IVF)& Intracytoplasmic sperm injection (ICSI)
Center for Studies, Consultation, and Community Service. The Hashemite University, Zarqa, Jordan, October 2007 (36 hours)
License details
Address: The Ministry of Health, Amman- Jordan
Professional status: Registered Medical laboratory technician
Registration #: 70002/984
Registration date: 11/2/2007
Conferences and Workshops Attendance
Placental Mesenchymal Stem Cell from Characterization to Clinical Application, King Abdullah International Medical Research Center. Research
Promotion and Education Section, Kingdom of Saudi Arabia
National Guard Health Affairs (NGHA) 16-17 April 2012
The third international congress of the jordanian society for fertility and genetic, 8th -10th April 2009
American Association for cancer research (AACR). Advances in Cancer Research: From the Laboratory to the clinic, King Hussein Bin Talal
Convention Center, Dead Sea, Jordan, March 16-19 2008
2nd Jordan Society of Pathology and Laboratory Medicine International Conferences, Le Royal Hotel, Amman, Jordan August 31st to
September 3rd 2006
Computer Skills and Qualifications:
-Microsoft office
- Statistical Analyses (SPSS, Statistica & prism)
-Internet
Languages:
- Arabic (mother tongue)
- English (very good; reading, spoken & written)
Professional Experience
1) Protein extraction and determination (Bradford and Lowry method)
2) Thiol assays (GSH/GSSG level).
3) Hormonal assay
4) Measurement the microphage activity (Nitric Oxide assay)
5) Lipid peroxidation assay (TBAR assay)
6) DNA extraction and evaluation DNA fragmentation by using gel electrophoresis and acridin orange
7) Routine H&E stain
8) Preparation electron microscope sample
9) Immunohistochemistry and Immunocytochemistry
10) Immunoblotting and Redox Western Blotting
11) Assessment and principles of management of IVF laboratory; semen preparation and assessment of sperm parameter and function; embryo
culture and cryopreservation in assisted reproduction; advanced laboratory techniques in assisted reproduction.
12) Assessment and principles of management of tissue culture laboratory: Isolation of cells, maintaining cells in culture, Manipulation of
cultured cells, Passaging cells, Transfection and transduction, determination cell Cytotoxicity and proliferation by using Cell proliferation assay (MTT assay), clonogenic survival assay and Preservation and Storage tissue culture cell
13) Preparation of the plant aqueous and alkaloid extract
14) Determination lethal dose, LD50 of any substance
15) Experimental models of pain in rat and mice: The antinociceptive activity of any agent by using hot plate and formalin tests, and paw edema test to study Anti-Inflammatory activity.
16) Laboratory Animal Handling Technique: mice and rat (Blood collection, Intraperitoneal Subcutaneous, intramuscular injection, and oral feeding etc)
References: Available upon request, and you can call or contact:
Dr.Maher Y. Abdalla Assistant Professor .Center for Cancer Research, NCI
National Naval Medical Center
Bldg. 8, Rm. 4155
8901 Wisconsin Ave.
Bethesda, MD 20889, USA
Tel: (301) 402-5159
Fax: (301) 496-0047
cell phone +962-795514343
maher_abdalla@yahoo.com
abdallamy@mail.nih.gov
Dr.Lubna H. Tahtamouni Assistant Professor. Dep. of Biological Sciences and Biotechnology Faculty of Sciences.The Hashemite University,
Zarqa, Jordan.
P.O. Box 150459, Zarqa 13115, Jordan
Phone: +962-5-3903333 Ext.5031
Fax :+962-5-3826613
cell phone +962-785701902
lubnatah@yahoo.com
Dr. Iman M. Ahmad Assistant Professor. Dep.of Radiography Faculty of Allied Health Sciences.The Hashemite University, Zarqa, Jordan.
P.O. Box 150459, Zarqa
Cell phone 0796162063
iman_maher@yahoo.com
Research Project
Application of Next Generation Sequencing technologies to a group of patients with different rare undiagnosed diseases.
Research plan and objectives: The identification of the molecular basis of a number of disorders has become the basis for the management of the disease itself and for performing clinical trials on patients homogeneous not so much on the basis of the clinical phenotype but rather on the specific gene alteration. These trials, in turn, make it possible to identify categories of drugs designed to suppress the pathological effects caused by the altered protein pathway. Obviously, the identification of the causative mutation in the patient allows the expansion of the investigation to his/her at risk family members who can therefore be treated in asymptomatic stages or in stages in which the disease is still mild. Most importantly and more pragmatically, it allows to perform prenatal diagnosis so to recognize fetuses at risk to be affected by the disease and take the proper decisions. Currently, the diagnosis of genetic mutations is possible for large numbers of patients and in real time thanks to the new approaches to gene sequencing (next generation sequencing) allowing sequencing of megabases of DNA at a cost even lower than 1000 € per patient and, therefore, much more convenient than the one requested for the analysis of a single gene with the Sanger method. The identification of genetic mutations associated with nephronophthisis could be a good example of a new medicine based on new diagnostic and therapeutic approaches. Nephronophthisis is the most frequent cause of polycystic kidney with loss of renal function and subsequent dialysis or transplantation by the third decade of life. The nephronophthisis fall into the category of the ciliopathies and are associated with mutations of at least 10 different genes. The construction of next generation platforms specifically dedicated to this disease makes it possible to identify the specific gene mutated and to implement treatment’s protocols in the family and still asymptomatic mutation carriers.
Methodologies: Patients DNAs will be evaluated by using next generation sequencing
either whole exome or targeted panels. The research takes advantage of two high throughput sequencers, Genome Analyzer IIx and MiSeq (both provided by Illumina) that are currently in use by the team of Prof. Zuffardi
Publications
Peer-Reviewed Publications
* Noor H. Mustafa, Iman M. Hassan, Iman M. Ahmad, Maher Y. Abdalla, Lubna H. Tahtamouni .Nandrolone Decanoate Administration to Male Rats
Induces Oxidative Stress, Seminiferous Tubules Abnormalities, and Sperm DNA Fragmentation. Jordan Journal of Biological 3, (4):165 - 174
* Maher Y. Abdalla, Iman M. Hassan, Noor H. Mustafa, Iman M. Ahmad, Lubna H.Tahtamouni (2010).Total Body Glutathione Depletion Induces
Oxidative Stress and Disrupts the Immune Function in Mice. Toxicological & Environmental Chemistry.1-14
*Iman M. Ahmad, Ibtihal, Noor H. Mustafa, Lubna H. Tahtamouni, Maher Y. Abdalla .(2010) 2DG enhances the susceptibility of breast cancer
cells to doxorubicin. Central European Journal of Biology . 5(6):739-748.
*Iman M. Ahmad , Maher Y. Abdalla , Noor H. Mustafa , Esam Y. Qnais , Fuad A. Abdulla.(2009) Datura Aqueous Leaf Extract Enhances
Cytotoxicity via Metabolic Oxidative Stress on Different Human Cancer Cells. Jordan Journal of Biological Sciences .2(1):9-14
* Tahtamouni LH, Mustafa NH, Alfaouri AA, Hassan IM, Abdalla MY, Yasin SR.(2008) Prevalence and Risk Factors for Anabolic-Androgenic Steroid
Abuse among Jordanian Collegiate Students and Athletes. European Journal of Public Health. 18(6): 661-665.
Name: Noor Hussein Mustafa
Nationality: Jordanian
Date & Place of Birth: June/02/1984. Kuwait.
Marital status: married
Nooroo20038@hotmail.com
Education
M.Sc. in Biological Sciences
The Hashemite University, Department of Biological Sciences and Biotechnology, Zarqa, Jordan January 2009 with an average of 3.65 Out of 4.00 rating: Excellent .
Thesis title: “The Effect of Supraphysiological Dosing of Anabolic –Androgenic Steroids on Chromatin and Expression of Androgen Receptor of the Rat Testis –Supporting Cells”
Supervisor: Dr. Lubna Tahtamouni, Co-Supervisor: Dr. Maher Abdalla
B.Sc. in Biological Sciences
Hashemite University, Department of Biological Sciences and Biotechnology, Zarqa, Jordan, June 2006 with an average of 3.73 Out of 4.00
rating: Excellent. get number 2nd In emamination Efficiency ofuniversity on all undergraduates Jordanian universities
Scientific Stream
Scientific Stream, Muscat, Oman, July 2002 with percentage average 96.51%
Professional Experience
Lecturer * King Saud bin Abdul Aziz for health science-UPPP September 2011 to September 2013
Lecturer * Department of Biological Sciences and Biotechnology, The Hashemite University February 2011 to June 2011
Lecturer * King Saud University, Kingdom of Saudi Arabia-ALKJARJ October 2009 to September 2010
Research Assistant Lab. Tissue culture, Department of Radiography, The Hashemite University, Zarqa, Jordan. December 2008 to October
2009
Teaching Assistant* Department of Biology, The Hashemite University, Zarqa, Jordan February 2009 to October
Student Teaching Assistant * Department of Biology, The Hashemite University, Zarqa, Jordan. September 2006 to June 2008 (four semester)
Research Assistant Tissue culture, immunology and Microtechniques lab. Department of Biology, The Hashemite University, Zarqa, Jordan.
September 2006 to June 2008
Research Assistant (when I was undergraduate I worked as volunteer) Neuroscience lab. Department of Biology, The Hashemite University, Zarqa, Jordan February 2006 to October 2006
*Teaching Experience
2006 to present – Immunology, Tissue Culture Techniques, Developmental Biology, Applied Developmental Biology, Gene Expression,
Microbiology, Recombenant DNA Techniques, Microtechniques, Hematolgy, Biochimistry , Chordates ,Physiology , Cell Biology , General Practical Biology (1); and General Practical Biology (2)
Training course
(video education training tutorials) http://www.knowledgecity.com
October 2011 to May 2012
1-The Art of Team Building
2- Problem Solving & Decision Making
3- The Art of Motivation
4- Time Management
5- Strategic Thinking
6- The Art of Negotiation
7- Effective Communication
8- Gender Communication
9- Generational Communication
10- Group Communication
11- Managing and Supervising
12- Elements of Leadership and Supervision
13- Intercultural Communication
14- Interpersonal Communication National guard health affairs
Medical terminology
National Guard Health Affairs (NGHA)\languish training February 2012 to May 2012
1-Methodolgy of sperm retrival in non obstructive azoospermia
2-Oocyte and ovarian tissue freezing
The Farah hospital on the 8th of April 2009 on the 8th of April 2009
Immunohistochemistry
The Higher Council For Science And Technology In Association With The Jordan Company For Antibody Production (MONOJO). October 2008 (24 hours)
Principles and Practices of Assisted Reproduction: In vitro fertilization (IVF)& Intracytoplasmic sperm injection (ICSI)
Center for Studies, Consultation, and Community Service. The Hashemite University, Zarqa, Jordan, October 2007 (36 hours)
License details
Address: The Ministry of Health, Amman- Jordan
Professional status: Registered Medical laboratory technician
Registration #: 70002/984
Registration date: 11/2/2007
Conferences and Workshops Attendance
Placental Mesenchymal Stem Cell from Characterization to Clinical Application, King Abdullah International Medical Research Center. Research
Promotion and Education Section, Kingdom of Saudi Arabia
National Guard Health Affairs (NGHA) 16-17 April 2012
The third international congress of the jordanian society for fertility and genetic, 8th -10th April 2009
American Association for cancer research (AACR). Advances in Cancer Research: From the Laboratory to the clinic, King Hussein Bin Talal
Convention Center, Dead Sea, Jordan, March 16-19 2008
2nd Jordan Society of Pathology and Laboratory Medicine International Conferences, Le Royal Hotel, Amman, Jordan August 31st to
September 3rd 2006
Computer Skills and Qualifications:
-Microsoft office
- Statistical Analyses (SPSS, Statistica & prism)
-Internet
Languages:
- Arabic (mother tongue)
- English (very good; reading, spoken & written)
Professional Experience
1) Protein extraction and determination (Bradford and Lowry method)
2) Thiol assays (GSH/GSSG level).
3) Hormonal assay
4) Measurement the microphage activity (Nitric Oxide assay)
5) Lipid peroxidation assay (TBAR assay)
6) DNA extraction and evaluation DNA fragmentation by using gel electrophoresis and acridin orange
7) Routine H&E stain
8) Preparation electron microscope sample
9) Immunohistochemistry and Immunocytochemistry
10) Immunoblotting and Redox Western Blotting
11) Assessment and principles of management of IVF laboratory; semen preparation and assessment of sperm parameter and function; embryo
culture and cryopreservation in assisted reproduction; advanced laboratory techniques in assisted reproduction.
12) Assessment and principles of management of tissue culture laboratory: Isolation of cells, maintaining cells in culture, Manipulation of
cultured cells, Passaging cells, Transfection and transduction, determination cell Cytotoxicity and proliferation by using Cell proliferation assay (MTT assay), clonogenic survival assay and Preservation and Storage tissue culture cell
13) Preparation of the plant aqueous and alkaloid extract
14) Determination lethal dose, LD50 of any substance
15) Experimental models of pain in rat and mice: The antinociceptive activity of any agent by using hot plate and formalin tests, and paw edema test to study Anti-Inflammatory activity.
16) Laboratory Animal Handling Technique: mice and rat (Blood collection, Intraperitoneal Subcutaneous, intramuscular injection, and oral feeding etc)
References: Available upon request, and you can call or contact:
Dr.Maher Y. Abdalla Assistant Professor .Center for Cancer Research, NCI
National Naval Medical Center
Bldg. 8, Rm. 4155
8901 Wisconsin Ave.
Bethesda, MD 20889, USA
Tel: (301) 402-5159
Fax: (301) 496-0047
cell phone +962-795514343
maher_abdalla@yahoo.com
abdallamy@mail.nih.gov
Dr.Lubna H. Tahtamouni Assistant Professor. Dep. of Biological Sciences and Biotechnology Faculty of Sciences.The Hashemite University,
Zarqa, Jordan.
P.O. Box 150459, Zarqa 13115, Jordan
Phone: +962-5-3903333 Ext.5031
Fax :+962-5-3826613
cell phone +962-785701902
lubnatah@yahoo.com
Dr. Iman M. Ahmad Assistant Professor. Dep.of Radiography Faculty of Allied Health Sciences.The Hashemite University, Zarqa, Jordan.
P.O. Box 150459, Zarqa
Cell phone 0796162063
iman_maher@yahoo.com
Research Project
Application of Next Generation Sequencing technologies to a group of patients with different rare undiagnosed diseases.
Research plan and objectives: The identification of the molecular basis of a number of disorders has become the basis for the management of the disease itself and for performing clinical trials on patients homogeneous not so much on the basis of the clinical phenotype but rather on the specific gene alteration. These trials, in turn, make it possible to identify categories of drugs designed to suppress the pathological effects caused by the altered protein pathway. Obviously, the identification of the causative mutation in the patient allows the expansion of the investigation to his/her at risk family members who can therefore be treated in asymptomatic stages or in stages in which the disease is still mild. Most importantly and more pragmatically, it allows to perform prenatal diagnosis so to recognize fetuses at risk to be affected by the disease and take the proper decisions. Currently, the diagnosis of genetic mutations is possible for large numbers of patients and in real time thanks to the new approaches to gene sequencing (next generation sequencing) allowing sequencing of megabases of DNA at a cost even lower than 1000 € per patient and, therefore, much more convenient than the one requested for the analysis of a single gene with the Sanger method. The identification of genetic mutations associated with nephronophthisis could be a good example of a new medicine based on new diagnostic and therapeutic approaches. Nephronophthisis is the most frequent cause of polycystic kidney with loss of renal function and subsequent dialysis or transplantation by the third decade of life. The nephronophthisis fall into the category of the ciliopathies and are associated with mutations of at least 10 different genes. The construction of next generation platforms specifically dedicated to this disease makes it possible to identify the specific gene mutated and to implement treatment’s protocols in the family and still asymptomatic mutation carriers.
Methodologies: Patients DNAs will be evaluated by using next generation sequencing
either whole exome or targeted panels. The research takes advantage of two high throughput sequencers, Genome Analyzer IIx and MiSeq (both provided by Illumina) that are currently in use by the team of Prof. Zuffardi
Publications
Peer-Reviewed Publications
* Noor H. Mustafa, Iman M. Hassan, Iman M. Ahmad, Maher Y. Abdalla, Lubna H. Tahtamouni .Nandrolone Decanoate Administration to Male Rats
Induces Oxidative Stress, Seminiferous Tubules Abnormalities, and Sperm DNA Fragmentation. Jordan Journal of Biological 3, (4):165 - 174
* Maher Y. Abdalla, Iman M. Hassan, Noor H. Mustafa, Iman M. Ahmad, Lubna H.Tahtamouni (2010).Total Body Glutathione Depletion Induces
Oxidative Stress and Disrupts the Immune Function in Mice. Toxicological & Environmental Chemistry.1-14
*Iman M. Ahmad, Ibtihal, Noor H. Mustafa, Lubna H. Tahtamouni, Maher Y. Abdalla .(2010) 2DG enhances the susceptibility of breast cancer
cells to doxorubicin. Central European Journal of Biology . 5(6):739-748.
*Iman M. Ahmad , Maher Y. Abdalla , Noor H. Mustafa , Esam Y. Qnais , Fuad A. Abdulla.(2009) Datura Aqueous Leaf Extract Enhances
Cytotoxicity via Metabolic Oxidative Stress on Different Human Cancer Cells. Jordan Journal of Biological Sciences .2(1):9-14
* Tahtamouni LH, Mustafa NH, Alfaouri AA, Hassan IM, Abdalla MY, Yasin SR.(2008) Prevalence and Risk Factors for Anabolic-Androgenic Steroid
Abuse among Jordanian Collegiate Students and Athletes. European Journal of Public Health. 18(6): 661-665.
