Universitą degli studi di Pavia
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Lombardi's cv
Curriculum
PERSONAL INFORMATION
First Name: Anita
Surname: Lombardi
Date of birth: 3 Agosto 1988
Place of birth: Isernia IS, Italy
Nationality: Italian
Gender: Female
Adress: c/da Vazzieri Poggio Verde 86010 Ferrazzano (CB) Italy
E-mail: anita-88@live.it
Telephone number: +39.388. 3425395
EDUCATION
November 2012
Qualifying examination to biologist register (32/40)
October 2012
Master’s degree in Medical Biotechnologies at University of Siena. (110/110 with honors)
Thesis:” Genetic reprogramming of FOXG1 mutated fibroblasts into induced pluripotent stem cells”
September 2010
Bachelor’s Degree in Biotechnologies at University of Siena. (110/110 with honors)
Thesis: “ Study of the action of different adjuvants to improve the priming of T cells”
July 2007
High School Leaving Certificate at Liceo Classico “M. Pagano” of Campobasso (CB) (90/100)
2005
“First certificate in English (FCE)”, Accademia Britannica Campobasso, Italy
2004
“Preliminary English Test (PET)”, Accademia Britannica Campobasso, Italy
RESEARCH EXPERIENCE
March 2013 at present: CNR fellow at the IGM-CNR (Pavia) as part of the project ” Analysis of the epigenetics modifications that affects the integrity and stability of human genome” supervised by Dr. Donata Orioli.
October 2011-October 2012: Research training as ungraduate student at the University of Siena, Laboratory of Medical Genetics working on the reprogramming and characterization of induced pluripotent stem cells (iPS) derived from FOXG1 mutated fibroblasts. Supervisors: Dr. Francesca Mari and Dr. Ilaria Meloni.
March 2010- September 2010: research training as ungraduate student at the University of Siena, Laboratory of Microbiology working on the effects of different adjuvants to improve the priming of T cells. Supervisors: Dr. Donata Medaglini and Dr. Annalisa Ciabattini.
RESEARCH ACTIVITY:
March 2013-at present
Laboratory of Human Genomics, Institute of Molecular Genetics- National Research Council, Pavia-Italy.
Identification of epigenetics modifications of the genome associated with trichothiodystrophy.
The research activity has been focused on a complex ereditary disorder, trichothiodystrophy (TTD) characterised by developmental and neurological anomalies. This disorder is defective in nucleotide excision repair (NER), the only DNA repair pathway devoted to the removal of UV.induced lesions in human cells. Mutations in three subunits of the general transcription factor TFIIH are responsible for TTD. By using primary dermal fibroblasts from TTD patients I’m dissecting the TFIIH-dependent transcription alterations specific for TTD as well as the role of TFIIH in chromatin remodelling by using genome wide approaches such as RNAseq and ChIPseq.
October 2011-October 2012
Laboratory of Medical Genetics, University of Siena
Genetic reprogramming of FOXG1 mutated fibroblasts into induced pluripotent stem cells
During her ungraduated studies, she focused on the RETT syndrome, a progressive neurodevelopmental disorder that rapresent one of the most common causes of mental retardation in females. RETT is caused by mutations in one of the three genes MECP2, CDKL5 and FOXG1. She reprogrammed primary dermal fibroblasts from a patient mutated in FOXG1, into induced pluripotent stem cells (iPS) through a lentiviral infection with the four reprogramming factors ( C-Myc, Oct-4, Klf-4 and Sox-2). She characterised the iPS cells by confirming the expression ofpluripotent markers through immunostainig analysis and the reactivation of specific endogenous genesby Real-time PCR. The pluripotency of iPS cells was evaluated by investigating their ability to differentiate into embryonic bodies.
March 2010- September 2010
Laboratory of Microbiology, University of Siena
Study of the action of different adjuvants to improve the priming of T cells
During the undergraduate studies she performed experiments to test the effects of two different adjuvants, CpG and α-Gal-Cer in improving the priming of T cellsderived from OT-I and OT-II transgenic mice. She contributed to this study by identifying a reduced amount of activated T cells in mice treated with the antigen compared to mice treated with the antigen plus adjuvants. For this analysis she used flowcytometry and cell-sorting.
SCIENTIFIC ACTIVITY
Meeting and course attendance:
May 17, 2013: 14th workshop “Epigenetica e staminali” Unistem, Palazzo Greppi, Milan
May 14, 29013: Convegno congiunto IGM, Bologna
December 6, 2008: “ Percorso preventive assistenziale al cittadino fumatore”, University of Siena
ACQUIRED KNOWLEDGE
Laboratory of Human Genomics, Institute of Molecular Genetics- National Research Council, Pavia-Italy.
-maintenance and propagation of primary cell cultures;
-extraction, purification and quantification of nucleic acids;
-RNA quantitative amplification methods (real-time RT-PCR)
-chromatin immunoprecipitation (ChIP)
Laboratory of Medical Genetics, Faculty of Medical Biotechnologies, University of Siena, Italy
-preparation of Lentiviral transduction particle;
-transduction of human primary dermal fibroblasts and cells reprogramming;
-IPS cells differentiation;
-immunofluorescence and microscopy analysis;
-DNA sequencing
Laboratory of microbiology, Faculty of Biotechnology, University of Siena, Italy
-Management of transgenic mice and in vivo immunization;
-Flowcytometry and ELISA;
-B and T cells purification, RT-PCR analysis.
PERSONAL SKILLS AND COMPETENCES
- LANGUAGES
Italian: mother tongue
English: Upper-Intermediate, good in both written and spoken. FCE, First Certificate English, Level B2.
- COMPUTER SKILLS
Good Knowledge of Windows XP/Vista and Macintosh. Competent with Microsoft Office Programs: Word, Excel, Power Point, Outlook Express. Knowledge of Photoshop. Excellent use of the web and social networks.
Research Project
The research activity has been focused on a complex ereditary disorder, trichothiodystrophy (TTD), characterised by developmental and neuroligical anomalies. This disorder is defective in nucleotide excision repair (NER), the only DNA repair pathway devoted to the removal of UV-induced lesions in human cells. Mutation in three subunits of the general trascriptional factor TFIIH are responsible of TTD. By using primary dermal fibroblasts from TTD patients, I’m dissecting the TFIIH-dependent transcription alterations specific for TTD as well as the role of TFIIH in chromatin remodelling by using genome wide approches such as RNA seq and ChIPseq.
Publications
Abstracts
-Lombardi Anita, Arseni Lavinia, Lanzafame Manuela, Biamonti Giuseppe, Stefanini Miria and Orioli Donata. TFIIH- mutated cells as a model system to dissect the multiple rules of TFIIH in chromatin dynamics. Epigen Workshop, 13-16 October 2013 Capri.
-Arseni Lavinia, Lombardi Anita, Stefanini Miria and Orioli Donata. (2013) TTD transcriptional defects are responsible for extracellular matrix alterations. Convegno congiunto IGM Bologna, May 14- Pavia, Italy.
PERSONAL INFORMATION
First Name: Anita
Surname: Lombardi
Date of birth: 3 Agosto 1988
Place of birth: Isernia IS, Italy
Nationality: Italian
Gender: Female
Adress: c/da Vazzieri Poggio Verde 86010 Ferrazzano (CB) Italy
E-mail: anita-88@live.it
Telephone number: +39.388. 3425395
EDUCATION
November 2012
Qualifying examination to biologist register (32/40)
October 2012
Master’s degree in Medical Biotechnologies at University of Siena. (110/110 with honors)
Thesis:” Genetic reprogramming of FOXG1 mutated fibroblasts into induced pluripotent stem cells”
September 2010
Bachelor’s Degree in Biotechnologies at University of Siena. (110/110 with honors)
Thesis: “ Study of the action of different adjuvants to improve the priming of T cells”
July 2007
High School Leaving Certificate at Liceo Classico “M. Pagano” of Campobasso (CB) (90/100)
2005
“First certificate in English (FCE)”, Accademia Britannica Campobasso, Italy
2004
“Preliminary English Test (PET)”, Accademia Britannica Campobasso, Italy
RESEARCH EXPERIENCE
March 2013 at present: CNR fellow at the IGM-CNR (Pavia) as part of the project ” Analysis of the epigenetics modifications that affects the integrity and stability of human genome” supervised by Dr. Donata Orioli.
October 2011-October 2012: Research training as ungraduate student at the University of Siena, Laboratory of Medical Genetics working on the reprogramming and characterization of induced pluripotent stem cells (iPS) derived from FOXG1 mutated fibroblasts. Supervisors: Dr. Francesca Mari and Dr. Ilaria Meloni.
March 2010- September 2010: research training as ungraduate student at the University of Siena, Laboratory of Microbiology working on the effects of different adjuvants to improve the priming of T cells. Supervisors: Dr. Donata Medaglini and Dr. Annalisa Ciabattini.
RESEARCH ACTIVITY:
March 2013-at present
Laboratory of Human Genomics, Institute of Molecular Genetics- National Research Council, Pavia-Italy.
Identification of epigenetics modifications of the genome associated with trichothiodystrophy.
The research activity has been focused on a complex ereditary disorder, trichothiodystrophy (TTD) characterised by developmental and neurological anomalies. This disorder is defective in nucleotide excision repair (NER), the only DNA repair pathway devoted to the removal of UV.induced lesions in human cells. Mutations in three subunits of the general transcription factor TFIIH are responsible for TTD. By using primary dermal fibroblasts from TTD patients I’m dissecting the TFIIH-dependent transcription alterations specific for TTD as well as the role of TFIIH in chromatin remodelling by using genome wide approaches such as RNAseq and ChIPseq.
October 2011-October 2012
Laboratory of Medical Genetics, University of Siena
Genetic reprogramming of FOXG1 mutated fibroblasts into induced pluripotent stem cells
During her ungraduated studies, she focused on the RETT syndrome, a progressive neurodevelopmental disorder that rapresent one of the most common causes of mental retardation in females. RETT is caused by mutations in one of the three genes MECP2, CDKL5 and FOXG1. She reprogrammed primary dermal fibroblasts from a patient mutated in FOXG1, into induced pluripotent stem cells (iPS) through a lentiviral infection with the four reprogramming factors ( C-Myc, Oct-4, Klf-4 and Sox-2). She characterised the iPS cells by confirming the expression ofpluripotent markers through immunostainig analysis and the reactivation of specific endogenous genesby Real-time PCR. The pluripotency of iPS cells was evaluated by investigating their ability to differentiate into embryonic bodies.
March 2010- September 2010
Laboratory of Microbiology, University of Siena
Study of the action of different adjuvants to improve the priming of T cells
During the undergraduate studies she performed experiments to test the effects of two different adjuvants, CpG and α-Gal-Cer in improving the priming of T cellsderived from OT-I and OT-II transgenic mice. She contributed to this study by identifying a reduced amount of activated T cells in mice treated with the antigen compared to mice treated with the antigen plus adjuvants. For this analysis she used flowcytometry and cell-sorting.
SCIENTIFIC ACTIVITY
Meeting and course attendance:
May 17, 2013: 14th workshop “Epigenetica e staminali” Unistem, Palazzo Greppi, Milan
May 14, 29013: Convegno congiunto IGM, Bologna
December 6, 2008: “ Percorso preventive assistenziale al cittadino fumatore”, University of Siena
ACQUIRED KNOWLEDGE
Laboratory of Human Genomics, Institute of Molecular Genetics- National Research Council, Pavia-Italy.
-maintenance and propagation of primary cell cultures;
-extraction, purification and quantification of nucleic acids;
-RNA quantitative amplification methods (real-time RT-PCR)
-chromatin immunoprecipitation (ChIP)
Laboratory of Medical Genetics, Faculty of Medical Biotechnologies, University of Siena, Italy
-preparation of Lentiviral transduction particle;
-transduction of human primary dermal fibroblasts and cells reprogramming;
-IPS cells differentiation;
-immunofluorescence and microscopy analysis;
-DNA sequencing
Laboratory of microbiology, Faculty of Biotechnology, University of Siena, Italy
-Management of transgenic mice and in vivo immunization;
-Flowcytometry and ELISA;
-B and T cells purification, RT-PCR analysis.
PERSONAL SKILLS AND COMPETENCES
- LANGUAGES
Italian: mother tongue
English: Upper-Intermediate, good in both written and spoken. FCE, First Certificate English, Level B2.
- COMPUTER SKILLS
Good Knowledge of Windows XP/Vista and Macintosh. Competent with Microsoft Office Programs: Word, Excel, Power Point, Outlook Express. Knowledge of Photoshop. Excellent use of the web and social networks.
Research Project
The research activity has been focused on a complex ereditary disorder, trichothiodystrophy (TTD), characterised by developmental and neuroligical anomalies. This disorder is defective in nucleotide excision repair (NER), the only DNA repair pathway devoted to the removal of UV-induced lesions in human cells. Mutation in three subunits of the general trascriptional factor TFIIH are responsible of TTD. By using primary dermal fibroblasts from TTD patients, I’m dissecting the TFIIH-dependent transcription alterations specific for TTD as well as the role of TFIIH in chromatin remodelling by using genome wide approches such as RNA seq and ChIPseq.
Publications
Abstracts
-Lombardi Anita, Arseni Lavinia, Lanzafame Manuela, Biamonti Giuseppe, Stefanini Miria and Orioli Donata. TFIIH- mutated cells as a model system to dissect the multiple rules of TFIIH in chromatin dynamics. Epigen Workshop, 13-16 October 2013 Capri.
-Arseni Lavinia, Lombardi Anita, Stefanini Miria and Orioli Donata. (2013) TTD transcriptional defects are responsible for extracellular matrix alterations. Convegno congiunto IGM Bologna, May 14- Pavia, Italy.
