Universitą degli studi di Pavia
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Esposito's cv
Curriculum
Personal information
Name
Marta Esposito
E-mail
marta.esposito01@ateneopv.it
Nationality
Italian, Polish
Date of birth
25/11/1988
Gender
Female
Education
Maj 2015 - Present
Junior member of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”.
November 2013 - Present
PhD in Genetics, Molecular and Cellular Biology, University of Pavia, XXIX cycle (2013/2016). Supervisor: Prof. Maria Rosalia Pasca
October 2011 – July 2013
Master Degree in Molecular Biology and Genetics, University of Pavia, Italy. Classification average: 110/110 cum laude. Title of thesis: “New antitubercular drugs activated by EthA”. Supervisor: Prof. Giovanna Riccardi.
October 2008 – July 2011
Degree in Biological Sciences, University of Pavia, Italy. Classification average: 110/110 cum laude. Title of thesis: “Caratterizzazione di cellule tumorali propagate a lungo termine in presenza di 3-aminobenzamide”. Supervisor: Dr. Chiara Mondello.
September 2002 – June 2007
High school diploma, Liceo Scientifico Biologico Blaise Pascal, Ovada (AL), Italy. Classification average: 100/100 cum laude.
Work experiences
November 2013 - Present
PhD in Genetics, Molecular and Cellular Biology at the Molecular Microbiology Laboratory, Department of Biology and Biotechnology, XXIX cycle (2013/2016), University of Pavia, Italy. Supervisor: Prof. Maria Rosalia Pasca.
October 2011 – July 2013
Internship for Master Degree in Molecular Biology and Genetics at the Molecular Microbiology Laboratory, Department of Biology and Biotechnology, University of Pavia, Italy. Supervisor: Prof. Giovanna Riccardi.
Subject of internship: characterization of the mechanisms of action and resistance of new antitubercular drugs.
September 2010 – July 2011
Internship for Degree in Biological Sciences at the Molecular Genetics Institute (CNR), University of Pavia, Italy. Supervisor: Dr. Chiara Mondello. Subject of internship: genomic instability and cellular transformation; analysis of expression levels of different proteins in different cellular lines by Western Blot experiments.
Didactic work
November-December 2015
Tutor in “Laboratorio integrato di Biologia Sperimentale (Microbiologia)” course, Biotechnology Degree, University of Pavia.
November 2014
Assistant supervisor for the experimental thesis “Purificazione e determinazione dell’attivitą enzimatica della CTP sintasi PyrG di Mycobacterium tuberculosis”, student Liliana Giordano, Degree in Biological Sciences.
November-December 2014
Tutor in “Laboratorio integrato di Biologia Sperimentale (Microbiologia)” course, Biotechnology Degree, University of Pavia.
Technical skills
Microbiological techniques
Liquid and solid media preparation, sterilization techniques (autoclaving, filtration, UV), bacterial cultures, streaks and single colonies isolation, competent cells preparation and plasmid transformation by electroporation, minimal inhibitory concentration (MIC) determination of antibiotics and other compounds for Escherichia coli and Mycobacterium smegmatis.
Molecular Biology techniques
Plasmidic DNA extraction and purification, gene cloning, PCR. Expression and purification of recombinant proteins; SDS – Page. Protein extraction and Western blot.
Biochemical techniques
Expression and purification of recombinant proteins; SDS – Page. Determination of proteins enzymatic activity with the use of a spectrophotometer. Kinetics constant determination. Enzymatic inhibition assays. Oxygraph system. Liquid-ESI Mass Spectrometry.
Bioinformatic techniques
Analysis of chromatograms from Sanger sequencing; use of websites and software for annotation and analysis of genomic data (ClustalW, EXPASY proteomics server, Blastn, Blastp); analysis of genome sequencing data from Illumina platform using SolexaQA software (SolexaQA, DynamicTrim, LengthSort), BWA (bwa index, bwa aln, bwa samse), Soap.coverage, SAMtools (faidx, view, sort, pileup), VarScan (pileup2snp, pileup2indel), Tablet.
Computer skills
Competent with: Microsoft Windows XP/Vista/7, Microsoft OfficeTM software (Word TM, ExcelTM, PowerPointTM).
Courses and Masterclasses
International Famelab Masterclass in Science Communication, Isola Polvese, Perugia, Italy, 10-12 April 2015. Principal trainer: Quentin Cooper (BBC).
Languages
Polish: mother tongue
English: good level in speaking, writing and understanding
French: basic level
Research Project
Study of the mechanisms of action and resistance of new antitubercular drugs
Tuberculosis is an airborne, infectious disease caused by a bacterial pathogen, Mycobacterium tuberculosis. Since it remains a leading cause of mortality worldwide and due to the increasing spread of M. tuberculosis multidrug-resistant strains, the discovery of more effective drugs, together with the identification of novel drug targets, is an urgent need.
Our laboratory is a member of the European project “More Medicines for Tuberculosis” (VII FP) whose goals are the validation of at least five new drug targets pharmacologically and the discovery of at least one family of novel candidate antitubercular drugs. In particular, our laboratory is currently responsible for the characterization in terms of mechanisms of action and resistance of 19 molecules that are discovered within this project and already proven to be effective in models of active or latent tuberculosis.
Publications
Meetings and Conferences
• Chiarelli LR, Esposito M, Orena BS, Mori G, Pasca MR, Riccardi G. PyrG and Rv0579: two promising drug targets. XIth Consortium Meeting, More Medicines for Tuberculosis, Hotel Moderno, Pavia, Italy, 11-12 January 2016. (Oral communication).
• de Jesus Lopes Ribeiro AL, Mori G, Chiarelli LR, Esposito M, Albesa-Jove D, Urresti S, Comino N, Hartkoorn RC, Binda C, Cole ST, Makarov V, Guerin M, Pasca MR, Riccardi G. Rv2466c: a novel drug activator for new antitubercular compounds. 30th Meeting of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”, Ischia, Italy, 18-21 September 2013. Poster.
• Esposito M, Chiarelli LR, Mori G, Pasca MR, Riccardi G. New antitubercular prodrugs activated by EthA monooxygenase. 2nd Italian Experience in Biochemical Research: Young Minds at Work. Desenzano, Italy, 24-25 October 2014. (Oral communication).
• Esposito M, Mori G, Chiarelli LR, Orena BS, Pasca MR, Riccardi G. Mechanisms of action and resistance of 5-methyl-N-(4-nitrophenyl)-2-thiophenecarboxamide (7947882 compound). 9th Consortium Meeting, More Medicines for Tuberculosis, Gran Hotel Domine Bilbao, Bilbao, Spain, 8-9 January 2015. (Oral communication).
• Chiarelli LR,* Esposito M*, Mori G, Orena BS, Makarov V, Bellinzoni M, Degiacomi G, Boldrin F, Manganelli R, Mikušovį K, de Carvalho LPS, Cole ST, Riccardi G, Pasca MR. Mechanism of action of the new antitubercular thiophenecarboxamide derivatives: a multidisciplinary approach. 31st Meeting of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”, Ravenna, Italy, 23-26 September 2015. Poster (*equal contribution).
• Mori G*, Orena BS*, Chiarelli LR, Esposito M, Anatriello M, Makarov V, Pasca MR, Riccardi G. Characterization of a new antitubercular compound activated by Rv2466c. 31st Meeting of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”, Ravenna, Italy, 23-26 September 2015. Poster (*equal contribution).
Publications
• Mori G*, Chiarelli L*, Esposito M*, Makarov V*, Bellinzoni M, Hartkoorn RC, Degiacomi G, Boldrin F, Ekins S, de Jesus Lopes Ribeiro AL, Marino LB, Centįrovį, Svetlķkovį Z, Blasco J, Kazakova E., Lepioshkin A, Barilone N, Zanoni G, Porta A, Fondi M, Fani R, Baulard AR, Mikušovį K, Alzari PM, Manganelli R, de Carvalho LPS, Riccardi G, Cole ST, Pasca MR (2015) Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG. Chemistry and Biology. 22 (7): 917-27 (*equal contribution).
Personal information
Name
Marta Esposito
marta.esposito01@ateneopv.it
Nationality
Italian, Polish
Date of birth
25/11/1988
Gender
Female
Education
Maj 2015 - Present
Junior member of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”.
November 2013 - Present
PhD in Genetics, Molecular and Cellular Biology, University of Pavia, XXIX cycle (2013/2016). Supervisor: Prof. Maria Rosalia Pasca
October 2011 – July 2013
Master Degree in Molecular Biology and Genetics, University of Pavia, Italy. Classification average: 110/110 cum laude. Title of thesis: “New antitubercular drugs activated by EthA”. Supervisor: Prof. Giovanna Riccardi.
October 2008 – July 2011
Degree in Biological Sciences, University of Pavia, Italy. Classification average: 110/110 cum laude. Title of thesis: “Caratterizzazione di cellule tumorali propagate a lungo termine in presenza di 3-aminobenzamide”. Supervisor: Dr. Chiara Mondello.
September 2002 – June 2007
High school diploma, Liceo Scientifico Biologico Blaise Pascal, Ovada (AL), Italy. Classification average: 100/100 cum laude.
Work experiences
November 2013 - Present
PhD in Genetics, Molecular and Cellular Biology at the Molecular Microbiology Laboratory, Department of Biology and Biotechnology, XXIX cycle (2013/2016), University of Pavia, Italy. Supervisor: Prof. Maria Rosalia Pasca.
October 2011 – July 2013
Internship for Master Degree in Molecular Biology and Genetics at the Molecular Microbiology Laboratory, Department of Biology and Biotechnology, University of Pavia, Italy. Supervisor: Prof. Giovanna Riccardi.
Subject of internship: characterization of the mechanisms of action and resistance of new antitubercular drugs.
September 2010 – July 2011
Internship for Degree in Biological Sciences at the Molecular Genetics Institute (CNR), University of Pavia, Italy. Supervisor: Dr. Chiara Mondello. Subject of internship: genomic instability and cellular transformation; analysis of expression levels of different proteins in different cellular lines by Western Blot experiments.
Didactic work
November-December 2015
Tutor in “Laboratorio integrato di Biologia Sperimentale (Microbiologia)” course, Biotechnology Degree, University of Pavia.
November 2014
Assistant supervisor for the experimental thesis “Purificazione e determinazione dell’attivitą enzimatica della CTP sintasi PyrG di Mycobacterium tuberculosis”, student Liliana Giordano, Degree in Biological Sciences.
November-December 2014
Tutor in “Laboratorio integrato di Biologia Sperimentale (Microbiologia)” course, Biotechnology Degree, University of Pavia.
Technical skills
Microbiological techniques
Liquid and solid media preparation, sterilization techniques (autoclaving, filtration, UV), bacterial cultures, streaks and single colonies isolation, competent cells preparation and plasmid transformation by electroporation, minimal inhibitory concentration (MIC) determination of antibiotics and other compounds for Escherichia coli and Mycobacterium smegmatis.
Molecular Biology techniques
Plasmidic DNA extraction and purification, gene cloning, PCR. Expression and purification of recombinant proteins; SDS – Page. Protein extraction and Western blot.
Biochemical techniques
Expression and purification of recombinant proteins; SDS – Page. Determination of proteins enzymatic activity with the use of a spectrophotometer. Kinetics constant determination. Enzymatic inhibition assays. Oxygraph system. Liquid-ESI Mass Spectrometry.
Bioinformatic techniques
Analysis of chromatograms from Sanger sequencing; use of websites and software for annotation and analysis of genomic data (ClustalW, EXPASY proteomics server, Blastn, Blastp); analysis of genome sequencing data from Illumina platform using SolexaQA software (SolexaQA, DynamicTrim, LengthSort), BWA (bwa index, bwa aln, bwa samse), Soap.coverage, SAMtools (faidx, view, sort, pileup), VarScan (pileup2snp, pileup2indel), Tablet.
Computer skills
Competent with: Microsoft Windows XP/Vista/7, Microsoft OfficeTM software (Word TM, ExcelTM, PowerPointTM).
Courses and Masterclasses
International Famelab Masterclass in Science Communication, Isola Polvese, Perugia, Italy, 10-12 April 2015. Principal trainer: Quentin Cooper (BBC).
Languages
Polish: mother tongue
English: good level in speaking, writing and understanding
French: basic level
Research Project
Study of the mechanisms of action and resistance of new antitubercular drugs
Tuberculosis is an airborne, infectious disease caused by a bacterial pathogen, Mycobacterium tuberculosis. Since it remains a leading cause of mortality worldwide and due to the increasing spread of M. tuberculosis multidrug-resistant strains, the discovery of more effective drugs, together with the identification of novel drug targets, is an urgent need.
Our laboratory is a member of the European project “More Medicines for Tuberculosis” (VII FP) whose goals are the validation of at least five new drug targets pharmacologically and the discovery of at least one family of novel candidate antitubercular drugs. In particular, our laboratory is currently responsible for the characterization in terms of mechanisms of action and resistance of 19 molecules that are discovered within this project and already proven to be effective in models of active or latent tuberculosis.
Publications
Meetings and Conferences
• Chiarelli LR, Esposito M, Orena BS, Mori G, Pasca MR, Riccardi G. PyrG and Rv0579: two promising drug targets. XIth Consortium Meeting, More Medicines for Tuberculosis, Hotel Moderno, Pavia, Italy, 11-12 January 2016. (Oral communication).
• de Jesus Lopes Ribeiro AL, Mori G, Chiarelli LR, Esposito M, Albesa-Jove D, Urresti S, Comino N, Hartkoorn RC, Binda C, Cole ST, Makarov V, Guerin M, Pasca MR, Riccardi G. Rv2466c: a novel drug activator for new antitubercular compounds. 30th Meeting of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”, Ischia, Italy, 18-21 September 2013. Poster.
• Esposito M, Chiarelli LR, Mori G, Pasca MR, Riccardi G. New antitubercular prodrugs activated by EthA monooxygenase. 2nd Italian Experience in Biochemical Research: Young Minds at Work. Desenzano, Italy, 24-25 October 2014. (Oral communication).
• Esposito M, Mori G, Chiarelli LR, Orena BS, Pasca MR, Riccardi G. Mechanisms of action and resistance of 5-methyl-N-(4-nitrophenyl)-2-thiophenecarboxamide (7947882 compound). 9th Consortium Meeting, More Medicines for Tuberculosis, Gran Hotel Domine Bilbao, Bilbao, Spain, 8-9 January 2015. (Oral communication).
• Chiarelli LR,* Esposito M*, Mori G, Orena BS, Makarov V, Bellinzoni M, Degiacomi G, Boldrin F, Manganelli R, Mikušovį K, de Carvalho LPS, Cole ST, Riccardi G, Pasca MR. Mechanism of action of the new antitubercular thiophenecarboxamide derivatives: a multidisciplinary approach. 31st Meeting of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”, Ravenna, Italy, 23-26 September 2015. Poster (*equal contribution).
• Mori G*, Orena BS*, Chiarelli LR, Esposito M, Anatriello M, Makarov V, Pasca MR, Riccardi G. Characterization of a new antitubercular compound activated by Rv2466c. 31st Meeting of “Societą Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)”, Ravenna, Italy, 23-26 September 2015. Poster (*equal contribution).
Publications
• Mori G*, Chiarelli L*, Esposito M*, Makarov V*, Bellinzoni M, Hartkoorn RC, Degiacomi G, Boldrin F, Ekins S, de Jesus Lopes Ribeiro AL, Marino LB, Centįrovį, Svetlķkovį Z, Blasco J, Kazakova E., Lepioshkin A, Barilone N, Zanoni G, Porta A, Fondi M, Fani R, Baulard AR, Mikušovį K, Alzari PM, Manganelli R, de Carvalho LPS, Riccardi G, Cole ST, Pasca MR (2015) Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG. Chemistry and Biology. 22 (7): 917-27 (*equal contribution).
