Universitą degli studi di Pavia


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Siciliano's CV

Curriculum Vitae – Dr Paolo Siciliano

Telephone: +44(0)7856 279260 / +39 389 4292744
Address: Via Lazio 8, 26900 Lodi (LO) Italy
Email: paolo.siciliano@unipv.it
Nationality: Italian
Gender: Male
D.O.B: 24th April 1984

Education & Qualifications

University of Pavia, Italy
Rothamsted Research, Harpenden, UK 2008 –
PhD in Cellular Biology – In progress
Thesis: Pheromone perception in insects: pheromone purification and identification of pheromone binding proteins in the severe pest species Ceratitis capitata (Mediterranean fruit fly).
(Supervisor: Prof. Anna R. Malacrida; Co-Supervisor: Dr. Jing-Jiang Zhou)

University of Pavia, Italy 2006 – 2008
Masters Degree in Experimental and Applied Biology (Molecular Biology)
110/110 cum laude
Thesis student in the Laboratory of Molecular Biology of Insects of Economical Impact.
Thesis: Transcriptome analysis in Ceratitis capitata (Diptera, Tephritidae): individuation, characterization and expression profile analyses of genes involved in olfaction (Supervisor: Prof. Giuliano Gasperi)

University of Pavia, Italy 2003 – 2006
First Degree in Biotechnology
Thesis: Transgenic Ceratitis capitata (Diptera, Tephritidae): sperm-specific fluorescent protein expression (TurboGFP, DsRedExpress)
(Supervisor: Prof. Giuliano Gasperi)

Liceo Scientifico Satale G. Gandini, Lodi, Italy 1998 – 2003
High School Degree, (Maturitą Scientifica) 75/100
Trinity English Course 2001
Relevant Experience

Technical competence:

- Excellent knowledge in the main molecular biology techniques: PCR, iPCR, RT-PCR, cloning, electrophoresis, nucleic acid purification, probe design, blotting (Southern, Northern and Western), In situ hybridization, FISH, PRINS;

- Very good knowledge in Real Time PCR, both in relative and absolute quantification, with a very good knowledge of related softwares, such as MiniOpticon and CFX by BioRad;

- Very good knowledge in molecular biology related softwares, for sequence analyses, alignments, probe design, genome and trascriptome analyses (VectorNTI, AlignX, CLC Workbench, Muscle, T-Coffee, Praline, Primer3, Beacon Designer, and many others);

- Good knowledge in recombinant protein production and purification, preparation of constructs for expression in bacteria and yeast, gel filtration, ion-exchange chromatography;

- Basic knowledge of 3D protein structure and docking programmes (Phyre, Pymol)

- Good experience in insect transgenesis: construct preparation, micro-injection in embryos, screening and setting of transgenic insect lines;

- Insectary management and insect rearing;

- Excellent knowledge in MS-Office and Adobe applications and Internet use.

Other skills:
First language Italian; fluent in English.
Excellent ability to work in a team, motivated, open-minded, very outgoing, interested in learning new techniques and developing new ideas and projects.

Work experience
Molecular biology laboratory since 2005.
Store management, sales person, bar staff, public relations and event organization.

Sports (Football, Tennis, Swimming), music, cooking and fine cuisine, spending time with friends and meeting new people, travel.

Prof. Anna R. Malacrida Lab. of Molecular Biology of Insects of Economical Impact, Dept. Animal Biology University of Pavia 27100 Pavia, Italy malacrid@unipv.it
Prof. Giuliano Gasperi Lab. of Molecular Biology of Insects of Economical Impact, Dept. Animal Biology University of Pavia 27100 Pavia, Italy gasperi@unipv.it
Dr. Jing-Jiang Zhou Dept. of Biological Chemistry Rothamsted Research Harpenden, Herts, UK AL5 2JQ jing-jiang.zhou@rothamsted.ac.uk

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