Università degli studi di Pavia
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Degiacomi's CV
Name and Surname: Giulia Degiacomi
Place and date of birth: Cuneo (CN), 3rd March 1984
Nationality: Italian
Laboratory: +39 0382 985578
e-mail: giulia.degiacomi@unipv.it
EDUCATION
2003 High School Degree at Liceo Classico “G. Govone”, in Alba (CN), Italy
2006 First Level Degree in Biotecnology at Università degli Studi di Pavia, Italy
Title of the thesis: “Purificazione e preliminare caratterizzazione della FHIT bovina (Purification and preliminary characterization of bovine FHIT)”
Supervisor: Prof. Giovanna Valentini
Final mark: 110/110 cum laude
2008 Master Degree in Industrial Biotecnology at Università degli Studi di Pavia, Italy
Title of the thesis: “Preparazione e studi in vitro di nanotubi funzionalizzati per il rilascio controllato di farmaci (Preparation and in vitro characterization of functionalized nanotubes for controlled release of drugs)”
Supervisor: Prof. Piercarlo Mustarelli
Final mark: 110/110 cum laude
2008-present Doctorate Fellowship in "Genetic and Biomolecular Sciences" at Department of Genetics and Microbiology, Università degli Studi di Pavia
Supervisors: Prof. Giovanna Riccardi and Dr. Maria Rosalia Pasca
LABORATORY EXPERIENCE
2005-2006 Thesis internship at the Laboratory of Functional and Structural Biochemistry of Proteins at Department of Biochemistry “A. Castellani”, Università degli Studi di Pavia (supervisor: Prof. Giovanna Valentini).
2007-2008 Thesis internship at the Laboratory of Thin Films at Department of Physical Chemistry, Università degli Studi di Pavia (supervisor: Prof. PierCarlo Mustarelli)
2008-present PhD student at the Laboratory of General Microbiology at Department of Genetics and Microbiology at Università degli Studi di Pavia (supervisors: Prof. Giovanna Riccardi and Dr. Maria Rosalia Pasca).
Research topics:
- identification of the cellular target of new antitubercular drugs;
- characterization of M. smegmatis mutants resistant to new antitubercular compounds.
TECHNICAL SKILLS
Microbiological techniques
Liquid and solid media preparation, sterilization techniques (autoclaving, ovens, filtration, UV rays), bacterial cultures, streaks and single colonies isolation, Gram and Ziehl- Nielsen staining, competent cells preparation and plasmid transformation by electroporation or heat- shock, transformation by conjugation, replica plating, vital and total counting of cells, minimal inhibitory concentration (MIC) determination of antibiotics and other compounds for E. coli and M. smegmatis, MIC determination by antibiogram, bacterial identification by API tests.
Molecular Biology techniques
DNA purification, plasmid and genomic DNA extraction and purification, gene cloning, PCR. Site-direct mutagesis. Sequence analysis by bioinformatic techniques. Heterologous expression and purification of recombinant proteins in E. coli.
LANGUAGES
English good understanding
MEETING AND CONFERENCES
1. Pasca MR, Degiacomi G, de Jesus Lopes Ribeiro AL, Zara F, De Mori P, Mirrione M, Brerra R, Pagani L, Pucillo L, Troupioti P, Makarov V, Cole ST, Riccardi G. Caratterizzazione della regione responsabile della resistenza ai benzotiazinoni in isolati clinici di Mycobacterium tuberculosis. 37° Congresso nazionale di Microbiologia (SIM), 11-14/10/2009, Torino.
2. XXVIII SIMGBM National Meeting. Spoleto, Chiostro San Nicolò, 11-13/6/2009.
3. Buroni S, Manina G, Pasca MR, Ribeiro AL, Degiacomi G, De Rossi E, Riccardi G. (14- 15/04/2010) Decaprenylphosphoryl-beta-D-ribose 2’-epimerase from Mycobacterium tuberculosis is a magic drug target. Cortona Procarioti 2010, Cortona (AR).
4. Final Consortium Meeting, New Medicines for Tuberculosis. Davos, Switzerland, 16-17/2/2011.
5. First Consortium Meeting, More Medicines for Tuberculosis. Davos, Switzerland, 18/2/2011.
6. 5th AltUN Annual Meeting “Vaccines: Prevention is better than cure. Current Status and Future Prospects.”. Pavia, 11-12/3/2011.
7. de Jesus Lopes Ribeiro AL*, Degiacomi G*, Incandela ML, Molteni E, Mori G, Buroni S, Pasca MR. Mechanisms of resistance to benzothiazinones and dinitrobenzamides in Mycobacterium smegmatis. SIMGBM 29th National Meeting, 20-23/09/2011, Pisa (selected for oral comunication).
Place and date of birth: Cuneo (CN), 3rd March 1984
Nationality: Italian
Laboratory: +39 0382 985578
e-mail: giulia.degiacomi@unipv.it
EDUCATION
2003 High School Degree at Liceo Classico “G. Govone”, in Alba (CN), Italy
2006 First Level Degree in Biotecnology at Università degli Studi di Pavia, Italy
Title of the thesis: “Purificazione e preliminare caratterizzazione della FHIT bovina (Purification and preliminary characterization of bovine FHIT)”
Supervisor: Prof. Giovanna Valentini
Final mark: 110/110 cum laude
2008 Master Degree in Industrial Biotecnology at Università degli Studi di Pavia, Italy
Title of the thesis: “Preparazione e studi in vitro di nanotubi funzionalizzati per il rilascio controllato di farmaci (Preparation and in vitro characterization of functionalized nanotubes for controlled release of drugs)”
Supervisor: Prof. Piercarlo Mustarelli
Final mark: 110/110 cum laude
2008-present Doctorate Fellowship in "Genetic and Biomolecular Sciences" at Department of Genetics and Microbiology, Università degli Studi di Pavia
Supervisors: Prof. Giovanna Riccardi and Dr. Maria Rosalia Pasca
LABORATORY EXPERIENCE
2005-2006 Thesis internship at the Laboratory of Functional and Structural Biochemistry of Proteins at Department of Biochemistry “A. Castellani”, Università degli Studi di Pavia (supervisor: Prof. Giovanna Valentini).
2007-2008 Thesis internship at the Laboratory of Thin Films at Department of Physical Chemistry, Università degli Studi di Pavia (supervisor: Prof. PierCarlo Mustarelli)
2008-present PhD student at the Laboratory of General Microbiology at Department of Genetics and Microbiology at Università degli Studi di Pavia (supervisors: Prof. Giovanna Riccardi and Dr. Maria Rosalia Pasca).
Research topics:
- identification of the cellular target of new antitubercular drugs;
- characterization of M. smegmatis mutants resistant to new antitubercular compounds.
TECHNICAL SKILLS
Microbiological techniques
Liquid and solid media preparation, sterilization techniques (autoclaving, ovens, filtration, UV rays), bacterial cultures, streaks and single colonies isolation, Gram and Ziehl- Nielsen staining, competent cells preparation and plasmid transformation by electroporation or heat- shock, transformation by conjugation, replica plating, vital and total counting of cells, minimal inhibitory concentration (MIC) determination of antibiotics and other compounds for E. coli and M. smegmatis, MIC determination by antibiogram, bacterial identification by API tests.
Molecular Biology techniques
DNA purification, plasmid and genomic DNA extraction and purification, gene cloning, PCR. Site-direct mutagesis. Sequence analysis by bioinformatic techniques. Heterologous expression and purification of recombinant proteins in E. coli.
LANGUAGES
English good understanding
MEETING AND CONFERENCES
1. Pasca MR, Degiacomi G, de Jesus Lopes Ribeiro AL, Zara F, De Mori P, Mirrione M, Brerra R, Pagani L, Pucillo L, Troupioti P, Makarov V, Cole ST, Riccardi G. Caratterizzazione della regione responsabile della resistenza ai benzotiazinoni in isolati clinici di Mycobacterium tuberculosis. 37° Congresso nazionale di Microbiologia (SIM), 11-14/10/2009, Torino.
2. XXVIII SIMGBM National Meeting. Spoleto, Chiostro San Nicolò, 11-13/6/2009.
3. Buroni S, Manina G, Pasca MR, Ribeiro AL, Degiacomi G, De Rossi E, Riccardi G. (14- 15/04/2010) Decaprenylphosphoryl-beta-D-ribose 2’-epimerase from Mycobacterium tuberculosis is a magic drug target. Cortona Procarioti 2010, Cortona (AR).
4. Final Consortium Meeting, New Medicines for Tuberculosis. Davos, Switzerland, 16-17/2/2011.
5. First Consortium Meeting, More Medicines for Tuberculosis. Davos, Switzerland, 18/2/2011.
6. 5th AltUN Annual Meeting “Vaccines: Prevention is better than cure. Current Status and Future Prospects.”. Pavia, 11-12/3/2011.
7. de Jesus Lopes Ribeiro AL*, Degiacomi G*, Incandela ML, Molteni E, Mori G, Buroni S, Pasca MR. Mechanisms of resistance to benzothiazinones and dinitrobenzamides in Mycobacterium smegmatis. SIMGBM 29th National Meeting, 20-23/09/2011, Pisa (selected for oral comunication).
