Università degli studi di Pavia
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Maffia's curriculum
Curriculum
Antonio Maffia
antonio.maffia01@universitadipavia.it
Oct 2015 – present PhD student
Istituto di Genetica Molecolare (IGM – CNR) Pavia, Italy
Mar 2015 – May 2015 Erasmus Traineeship fellowship
Technical University Darmstadt, Department of Biology, Darmstadt, Germany
Oct 2013 – Jul 2015 Master Degree and traineeship
Università degli Studi di Pavia, Istituto di Genetica Molecolare (IGM – CNR) Pavia, Italy
Oct 2010 – Nov 2013 Bachelor Degree, Università degli Studi di Milano Bicocca, Milano, Italy
Publications
Abstracts
Dutto I, Maffia A, Tillhon M, Cazzalini O, Stivala LA, Prosperi E. p21 interacts with NER factors at DNA damage sites, before its degradation. DNA repair and Genome Stability in Chromatin Environment, Institute of Molecular Biology (IMB), Mainz. June 2015.
Research project
Establishment of a human cell line carrying PCNA K164 mutation through CRISPR/Cas technology
DNA repair is a highly dynamic process involving DNA transactions to exploit a successful repair process, upon DNA damage and thus ensuring genome stability for the cell. The majority of the players involved in numerous repair pathways have been characterized during previous years. One of the most fundamental and versatile proteins required during both DNA replication and DNA repair is PCNA (Proliferating Cell Nuclear Antigen). Acting as a sliding clamp, PCNA undergoes different post-translational modifications to allow complex molecular dynamics to occur, upon its binding. One of the major modifications of PCNA is its ubiquitylation on the lysine 164 residue, that occurs after DNA damage, such as UV irradiation. The pivotal role of PCNA ubiquitylation has already been assessed in the context of damage tolerance systems, like DNA translesion synthesis, and it is thought to be involved in the bypass of CPDs lesions on DNA. The aim of the project is to further investigate the role of PCNA ubiquitylation through the mutation of the specific target residue, K164. We propose to establish a number of stable cell lines, mutated in the residue, using the new CRISPR/Cas technology. The creation of such tool will help to further delineate the role of Ubi-PCNA in DNA replication and repair.
Antonio Maffia
antonio.maffia01@universitadipavia.it
Oct 2015 – present PhD student
Istituto di Genetica Molecolare (IGM – CNR) Pavia, Italy
Mar 2015 – May 2015 Erasmus Traineeship fellowship
Technical University Darmstadt, Department of Biology, Darmstadt, Germany
Oct 2013 – Jul 2015 Master Degree and traineeship
Università degli Studi di Pavia, Istituto di Genetica Molecolare (IGM – CNR) Pavia, Italy
Oct 2010 – Nov 2013 Bachelor Degree, Università degli Studi di Milano Bicocca, Milano, Italy
Publications
Abstracts
Dutto I, Maffia A, Tillhon M, Cazzalini O, Stivala LA, Prosperi E. p21 interacts with NER factors at DNA damage sites, before its degradation. DNA repair and Genome Stability in Chromatin Environment, Institute of Molecular Biology (IMB), Mainz. June 2015.
Research project
Establishment of a human cell line carrying PCNA K164 mutation through CRISPR/Cas technology
DNA repair is a highly dynamic process involving DNA transactions to exploit a successful repair process, upon DNA damage and thus ensuring genome stability for the cell. The majority of the players involved in numerous repair pathways have been characterized during previous years. One of the most fundamental and versatile proteins required during both DNA replication and DNA repair is PCNA (Proliferating Cell Nuclear Antigen). Acting as a sliding clamp, PCNA undergoes different post-translational modifications to allow complex molecular dynamics to occur, upon its binding. One of the major modifications of PCNA is its ubiquitylation on the lysine 164 residue, that occurs after DNA damage, such as UV irradiation. The pivotal role of PCNA ubiquitylation has already been assessed in the context of damage tolerance systems, like DNA translesion synthesis, and it is thought to be involved in the bypass of CPDs lesions on DNA. The aim of the project is to further investigate the role of PCNA ubiquitylation through the mutation of the specific target residue, K164. We propose to establish a number of stable cell lines, mutated in the residue, using the new CRISPR/Cas technology. The creation of such tool will help to further delineate the role of Ubi-PCNA in DNA replication and repair.
