Università degli studi di Pavia
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Pradella's curriculum
Curriculum
PERSONAL INFORMATION
Name: Davide
Surname: Pradella
Date of birth: 18TH April 1990
Nationality: Italian
Address: 17, Via Dante, 22015, Gravedona ed Uniti, Como, Italy
EDUCATION AND TRAINING
11/2014 - present: Phd program in Genetics, Molecular and Cellular Biology. University of Pavia, Italy
09/2009 - 05/2015: Undergraduate courses in Biomedical Sciences. Institute for Advanced Study (IUSS), Pavia, Italia.
Diploma di Licenza Thesis: Gene expression analysis of Thoracic Aortic Aneurysms associated with bicuspid aortic valves compared to tricuspid aortic valves: a source for novel potential biomarkers.
Supervisor: Prof. Orsetta Zuffardi
09/2012 - 07/2014: Master of Science in Molecular Biology and Genetics (110/110 cum laude). University of Pavia, Italy
Master thesis: Study of the alternative splicing of Par3, the central organizer of the apico-basal cellular polarity.
Supervisor: Dr. Claudia Ghigna
09/2009 - 07/2012: Bachelor degree in Biotechnology (110/110 cum laude). University of Pavia, Italy
Bachelor Thesis: Chromatography approaches for Irudin Variant 1 purification.
Supervisor: Dr. Claudia Binda
09/2004 - 07/2009: Maturità scientifica, Liceo Scientifico P. Nervi, Morbegno, Italy
EDUCATIONAL EXPERIENCES
12/11/2015: Training Course: "Sicurezza e Salute nei Luoghi di Lavoro ai sensi del D.Lgs. 81/08. University of Pavia, Italy
7/10/2015: Seminars RNAseq e Target Enrichment. BMR Genomics (Padua) c/o University of Pavia, Italy
14-17/04/2014: Theorical Course: “RNA Structure and Function”, International Centre For Genetic Engineering And Biotechnology (ICGEB)
05/2007 and 05/2008: CusMiBio project, (Centre University of Milan - School for Biosciences education). University of Milan, Italy
PERSONAL SKILLS AND COMPETENCES
Mother Tongue: Italian
Other Languages: English – Professional working proficiency
French – DELF B1 level (Certification of Ministère éducation nationale, France)
Social and organisational skills and competences:
- Team spirit
- Good ability to adapt to new and multicultural environments
- Capable of discussing on article and of explaining them to others
- Leadership and sense of organisation
Technical and artistic skills and competences:
Technical laboratory skills:
Nucleic acid and protein extraction, PCR, RT-PCR, q-PCR, Agarose and polyacrylamide gel electrophoresis, Eucaryotic cell and Bacterial Cultures, ability to work under aseptic conditions, cloning techniques, Western-Blot, Protein and DNA purification. Cell survival assay.
Bioinformatic tools and databases:
NCBI, EBI, OMIM, UCSC, Protein Data Bank, UniProt, BLAST, BLAT, Exonmine, Fasta DB, Esefinder, SFmap Hapmap, Vista Browser, DAVID, Gene Ontology, Genemania, String, miRBase, TargetScan, PicTar.
Scientific Softwares:
Papers2, Endnote, FinchTV, ImaJ, Qutemole, EasyChem.
Other Softwares:
Excel / Numbers, Word / Pages, Powerpoint / Keynote / Prezi, AutoCAD, Photoshop. Base knowledge of HTML anc C language for web design.
Use of instruments:
Optical microscope, spectrophotometer, thermal cycler, laminar flow and chemical fume hood, imaging systems.
SCHOLARSHIPS RECEIVED
- 2013/2014 INPS Scholarship - provided by “INPS” (Istituto nazionale della previdenza sociale)
- 2013: “Giuseppe Casella” Scholarship - provided by “Almo Collegio Borromeo”
- 2012: “Cesare Angelini” Scholarship - provided by “Associazione Alunni Almo Collegio Borromeo”
- 2009 - 2012: “Dote di residenzialità” Scholarship - provided by “Regione Lombardia”
PRIZES
Winner of XII Olympic Games of Verbal-Linguistic and Logical-mathematical
University of Siena, University of Bari, Polytechnic of Bari, Gioia Mathesis Foundation, Italy, 2002
Publications
Giampietro C, Deflorian G, Gallo S, Di Matteo A, Pradella D, Bonomi S, Belloni E, Nyqvist D, Quaranta V, Confalonieri S, Bertalot G, Orsenigo F, Pisati F, Ferrero E, Biamonti G, Fredrickx E, Taveggia C, Wyatt CD, Irimia M, Di Fiore PP,
Blencowe BJ, Dejana E, Ghigna C. The alternative splicing factor Nova2 regulates vascular development and lumen formation. Nat Commun. 2015; 6 : 8479.
Frisone P, Pradella D, Di Matteo A, Belloni E, Ghigna C, Paronetto MP. SAM68: Signal Transduction and RNA Metabolism in Human Cancer. Biomed Res Int. 2015; 2015 : 528954.
Abstracts and presentations
- Giampietro C, Gallo S, Deflorian G, Bonomi S, Di Matteo A, Belloni E, Quaranta V, Nyqvist D, Confalonieri S, Bertalot G, Pradella D, Fredrickx E, Biamonti G, Taveggia C, Irimia M, Di Fiore PP, Blencowe BJ, Dejana E, Ghigna C. "The alternative splicing factor Nova2 is a novel regulator of angiogenesis". SIICA (Società Italiana Immunologia, Immunologia Clinica e Allergologia). Angiogenesi: basi molecolari ed implicazioni terapeutiche, Certosa di Pontignano, Italy, 25-27 May 2015. Poster.
- Gallo S., Giampietro C., Bonomi S., Deflorian G., Di Matteo A., Belloni E., Quaranta V., Pradella D., Biamonti G., Dejana E. and Ghigna C. “The alternative splicing factor Nova2 is a novel regulator of angiogenesis”. Joint IGM Meeting, Pavia, Italy, 3 October 2014. Oral communication.
- Gallo S., Bonomi S., Giampietro C., Deflorian G., Di Matteo A., Pradella D., Dejana E. and Ghigna C. “Identification of a novel post-transcriptional regulator of angiogenesis”. Joint National Ph. D. Meeting 2013, Pesaro, Italy, 10-12 October 2013. Poster.
Research project
A GENOME-EDITING TOOL FOR THE STUDY OF THE ROLE OF ALTERNATIVE SPLICING DURING ANGIOGENESIS
Recently a new tool has revolutionized genetic engineering approaches in the genome manipulation field. This genome editing tool, known as CRISPR (Clustered, Regularly Interspaced, Short Palindromic Repeats), has been efficiently used for exogenous DNA integration (Knock-in), depletion of specific genes (Knock-out), silencing of specific targets (CRISPRi, Knock-down), manipulate the transcriptional activity without affecting the genome sequence and as imaging tool for the study of specific loci (CRISPR imaging).
Through the use of this technology we aim to generate murine endothelial cell (ECs) knock-out for different splicing factors involved in the angiogenesis process.
Next we will investigate the differences in splicing profiles of specific splicing factor-depleted ECs compared to wild-type ECs as control, with the final goal to identify novel specific splicing variants expressed in ECs during angiogenesis. In particular, through the use of Next-Generation Sequencing (NGS) approaches such as the Whole Transcriptome Shotgun Sequencing (WTSS o RNA-seq) we will focus on alternative splicing events occurring in genes codifying protein surface molecules in endothelial cells.
PERSONAL INFORMATION
Name: Davide
Surname: Pradella
Date of birth: 18TH April 1990
Nationality: Italian
Address: 17, Via Dante, 22015, Gravedona ed Uniti, Como, Italy
EDUCATION AND TRAINING
11/2014 - present: Phd program in Genetics, Molecular and Cellular Biology. University of Pavia, Italy
09/2009 - 05/2015: Undergraduate courses in Biomedical Sciences. Institute for Advanced Study (IUSS), Pavia, Italia.
Diploma di Licenza Thesis: Gene expression analysis of Thoracic Aortic Aneurysms associated with bicuspid aortic valves compared to tricuspid aortic valves: a source for novel potential biomarkers.
Supervisor: Prof. Orsetta Zuffardi
09/2012 - 07/2014: Master of Science in Molecular Biology and Genetics (110/110 cum laude). University of Pavia, Italy
Master thesis: Study of the alternative splicing of Par3, the central organizer of the apico-basal cellular polarity.
Supervisor: Dr. Claudia Ghigna
09/2009 - 07/2012: Bachelor degree in Biotechnology (110/110 cum laude). University of Pavia, Italy
Bachelor Thesis: Chromatography approaches for Irudin Variant 1 purification.
Supervisor: Dr. Claudia Binda
09/2004 - 07/2009: Maturità scientifica, Liceo Scientifico P. Nervi, Morbegno, Italy
EDUCATIONAL EXPERIENCES
12/11/2015: Training Course: "Sicurezza e Salute nei Luoghi di Lavoro ai sensi del D.Lgs. 81/08. University of Pavia, Italy
7/10/2015: Seminars RNAseq e Target Enrichment. BMR Genomics (Padua) c/o University of Pavia, Italy
14-17/04/2014: Theorical Course: “RNA Structure and Function”, International Centre For Genetic Engineering And Biotechnology (ICGEB)
05/2007 and 05/2008: CusMiBio project, (Centre University of Milan - School for Biosciences education). University of Milan, Italy
PERSONAL SKILLS AND COMPETENCES
Mother Tongue: Italian
Other Languages: English – Professional working proficiency
French – DELF B1 level (Certification of Ministère éducation nationale, France)
Social and organisational skills and competences:
- Team spirit
- Good ability to adapt to new and multicultural environments
- Capable of discussing on article and of explaining them to others
- Leadership and sense of organisation
Technical and artistic skills and competences:
Technical laboratory skills:
Nucleic acid and protein extraction, PCR, RT-PCR, q-PCR, Agarose and polyacrylamide gel electrophoresis, Eucaryotic cell and Bacterial Cultures, ability to work under aseptic conditions, cloning techniques, Western-Blot, Protein and DNA purification. Cell survival assay.
Bioinformatic tools and databases:
NCBI, EBI, OMIM, UCSC, Protein Data Bank, UniProt, BLAST, BLAT, Exonmine, Fasta DB, Esefinder, SFmap Hapmap, Vista Browser, DAVID, Gene Ontology, Genemania, String, miRBase, TargetScan, PicTar.
Scientific Softwares:
Papers2, Endnote, FinchTV, ImaJ, Qutemole, EasyChem.
Other Softwares:
Excel / Numbers, Word / Pages, Powerpoint / Keynote / Prezi, AutoCAD, Photoshop. Base knowledge of HTML anc C language for web design.
Use of instruments:
Optical microscope, spectrophotometer, thermal cycler, laminar flow and chemical fume hood, imaging systems.
SCHOLARSHIPS RECEIVED
- 2013/2014 INPS Scholarship - provided by “INPS” (Istituto nazionale della previdenza sociale)
- 2013: “Giuseppe Casella” Scholarship - provided by “Almo Collegio Borromeo”
- 2012: “Cesare Angelini” Scholarship - provided by “Associazione Alunni Almo Collegio Borromeo”
- 2009 - 2012: “Dote di residenzialità” Scholarship - provided by “Regione Lombardia”
PRIZES
Winner of XII Olympic Games of Verbal-Linguistic and Logical-mathematical
University of Siena, University of Bari, Polytechnic of Bari, Gioia Mathesis Foundation, Italy, 2002
Publications
Giampietro C, Deflorian G, Gallo S, Di Matteo A, Pradella D, Bonomi S, Belloni E, Nyqvist D, Quaranta V, Confalonieri S, Bertalot G, Orsenigo F, Pisati F, Ferrero E, Biamonti G, Fredrickx E, Taveggia C, Wyatt CD, Irimia M, Di Fiore PP,
Blencowe BJ, Dejana E, Ghigna C. The alternative splicing factor Nova2 regulates vascular development and lumen formation. Nat Commun. 2015; 6 : 8479.
Frisone P, Pradella D, Di Matteo A, Belloni E, Ghigna C, Paronetto MP. SAM68: Signal Transduction and RNA Metabolism in Human Cancer. Biomed Res Int. 2015; 2015 : 528954.
Abstracts and presentations
- Giampietro C, Gallo S, Deflorian G, Bonomi S, Di Matteo A, Belloni E, Quaranta V, Nyqvist D, Confalonieri S, Bertalot G, Pradella D, Fredrickx E, Biamonti G, Taveggia C, Irimia M, Di Fiore PP, Blencowe BJ, Dejana E, Ghigna C. "The alternative splicing factor Nova2 is a novel regulator of angiogenesis". SIICA (Società Italiana Immunologia, Immunologia Clinica e Allergologia). Angiogenesi: basi molecolari ed implicazioni terapeutiche, Certosa di Pontignano, Italy, 25-27 May 2015. Poster.
- Gallo S., Giampietro C., Bonomi S., Deflorian G., Di Matteo A., Belloni E., Quaranta V., Pradella D., Biamonti G., Dejana E. and Ghigna C. “The alternative splicing factor Nova2 is a novel regulator of angiogenesis”. Joint IGM Meeting, Pavia, Italy, 3 October 2014. Oral communication.
- Gallo S., Bonomi S., Giampietro C., Deflorian G., Di Matteo A., Pradella D., Dejana E. and Ghigna C. “Identification of a novel post-transcriptional regulator of angiogenesis”. Joint National Ph. D. Meeting 2013, Pesaro, Italy, 10-12 October 2013. Poster.
Research project
A GENOME-EDITING TOOL FOR THE STUDY OF THE ROLE OF ALTERNATIVE SPLICING DURING ANGIOGENESIS
Recently a new tool has revolutionized genetic engineering approaches in the genome manipulation field. This genome editing tool, known as CRISPR (Clustered, Regularly Interspaced, Short Palindromic Repeats), has been efficiently used for exogenous DNA integration (Knock-in), depletion of specific genes (Knock-out), silencing of specific targets (CRISPRi, Knock-down), manipulate the transcriptional activity without affecting the genome sequence and as imaging tool for the study of specific loci (CRISPR imaging).
Through the use of this technology we aim to generate murine endothelial cell (ECs) knock-out for different splicing factors involved in the angiogenesis process.
Next we will investigate the differences in splicing profiles of specific splicing factor-depleted ECs compared to wild-type ECs as control, with the final goal to identify novel specific splicing variants expressed in ECs during angiogenesis. In particular, through the use of Next-Generation Sequencing (NGS) approaches such as the Whole Transcriptome Shotgun Sequencing (WTSS o RNA-seq) we will focus on alternative splicing events occurring in genes codifying protein surface molecules in endothelial cells.
