Universitą degli studi di Pavia
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Pistono's curriculum
Curriculum
Personal informations
FIRST NAME / SURNAME: Cristiana Pistono
ADDRESS: Via Provinciale 34, 13888, Mongrando (BI), Italy
MOBILE PHONE: 0039 347 5119276
E-MAIL: pistono.cristiana@libero.it
NATIONALITY: Italian
DATE OF BIRTH: 27/04/1990
GENDER: Female
Education and training
November 2014 – present:
PhD in Genetics, Molecular and Cellular Biology, University of Pavia, XXX cycle (2014/2017).
Supervisor: Prof. Mariaclara Cuccia, Prof. Elena Raimondi.
November 2014:
Qualification to the profession of Biologist, University of Pavia, Italy.
October 2012 - July 2014:
Masters degree in Experimental and Applied Biology (program in Human Biology and Biomedical Sciences), University of Pavia, Italy.
Thesis title: Role of Hsp70 in multiple sclerosis: genetic analysis and ex vivo and in vitro study on peripheral blood mononucleate cells.
Supervisors: Prof. Mariaclara Cuccia, Dott. Chiara Boiocchi.
Final grade: 110/110 cum laude.
October 2009 - July 2012:
Bachelor Degree in Biological Sciences, University of Pavia, Italy.
Thesis title: Immunigenic approach to multiple sclerosis: how to set up a record of patients and begin the study of the polymorphism of Hsp genes.)
Supervisors: Prof. Mariaclara Cuccia, Dott. Chiara Boiocchi.
Final grade: 110/110 cum laude.
2009:
Scientific High School Diploma – Liceo Scientifico Tecnologico, Biella, Italy.
Final grade: 100/100.
Work experience
November 2014 – present: PhD in Genetics, Molecular and Cellular Biology, University of Pavia, XXX cycle (2014/2017).
Supervisor: Prof. Mariaclara Cuccia, Prof. Elena Raimondi.
October 2012 - July 2014: Internship for Masters Degree in Experimental and Applied Biology at the Laboratory of Immunogenetics, Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Italy.
2012: Internship for Bachelor Degree in Biological Sciences at the Laboratory of Immunogenetics, Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Italy.
Personal skills and competences
MOTHER TONGUE: Italian
OTHER LANGUAGE(S):
ENGLISH: Intermediate level
SPANISH: Intermediate level
Technical skills and competences
Separation of blood components by density gradient centrifugation, DNA extraction from blood through “GeneMATRIX Quick Blood Purification Kit”, molecular analysis by PCR and PCR-RFLP, agarose gel electrophoresis, polyacrilamide gel electrophoresis and western blotting, cell cultures of PBMCs, MTT assay for the evaluation of mitochondrial activity, RNA extraction with Trizol.
Computer skills and competences
Good knowledge of Windows operating systems, Microsoft Office™ software (Word™, Excel™, Power Point™), Internet, NCBI databases.
ECDL Licence.
Publications
Research project
GENETICS AND EPIGENETICS IN NEUROINFLAMMATION
This project will consider the role of oxidative stress involved in neurodegeneration and in the development of brain damage in multiple sclerosis. It will study the joint effects of protein Hsp70 (that is controlled by genes localised on the MHC class III region, on chromosome 6) and vitamin D (receptor encoded by a gene on chromosome 12) on the risk of developing the disease; risk defined by the interaction of genetic, environmental and epigenetic factors.
Multiple sclerosis (MS) is a multifactorial, disimmune disease, that affects the central nervous system and is characterised by an inflammatory process that leads to myelin damage. The pathogenesis of this disease is complex and involves different interconnected mechanisms. Furthermore different microRNAs, whose expression is altered in neurodegenerative disease, will be considered; the effects due to the action of pharmacological substances on patients, able to modify the expression levels of microRNAs, will be studied.
Personal informations
FIRST NAME / SURNAME: Cristiana Pistono
ADDRESS: Via Provinciale 34, 13888, Mongrando (BI), Italy
MOBILE PHONE: 0039 347 5119276
E-MAIL: pistono.cristiana@libero.it
NATIONALITY: Italian
DATE OF BIRTH: 27/04/1990
GENDER: Female
Education and training
November 2014 – present:
PhD in Genetics, Molecular and Cellular Biology, University of Pavia, XXX cycle (2014/2017).
Supervisor: Prof. Mariaclara Cuccia, Prof. Elena Raimondi.
November 2014:
Qualification to the profession of Biologist, University of Pavia, Italy.
October 2012 - July 2014:
Masters degree in Experimental and Applied Biology (program in Human Biology and Biomedical Sciences), University of Pavia, Italy.
Thesis title: Role of Hsp70 in multiple sclerosis: genetic analysis and ex vivo and in vitro study on peripheral blood mononucleate cells.
Supervisors: Prof. Mariaclara Cuccia, Dott. Chiara Boiocchi.
Final grade: 110/110 cum laude.
October 2009 - July 2012:
Bachelor Degree in Biological Sciences, University of Pavia, Italy.
Thesis title: Immunigenic approach to multiple sclerosis: how to set up a record of patients and begin the study of the polymorphism of Hsp genes.)
Supervisors: Prof. Mariaclara Cuccia, Dott. Chiara Boiocchi.
Final grade: 110/110 cum laude.
2009:
Scientific High School Diploma – Liceo Scientifico Tecnologico, Biella, Italy.
Final grade: 100/100.
Work experience
November 2014 – present: PhD in Genetics, Molecular and Cellular Biology, University of Pavia, XXX cycle (2014/2017).
Supervisor: Prof. Mariaclara Cuccia, Prof. Elena Raimondi.
October 2012 - July 2014: Internship for Masters Degree in Experimental and Applied Biology at the Laboratory of Immunogenetics, Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Italy.
2012: Internship for Bachelor Degree in Biological Sciences at the Laboratory of Immunogenetics, Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Italy.
Personal skills and competences
MOTHER TONGUE: Italian
OTHER LANGUAGE(S):
ENGLISH: Intermediate level
SPANISH: Intermediate level
Technical skills and competences
Separation of blood components by density gradient centrifugation, DNA extraction from blood through “GeneMATRIX Quick Blood Purification Kit”, molecular analysis by PCR and PCR-RFLP, agarose gel electrophoresis, polyacrilamide gel electrophoresis and western blotting, cell cultures of PBMCs, MTT assay for the evaluation of mitochondrial activity, RNA extraction with Trizol.
Computer skills and competences
Good knowledge of Windows operating systems, Microsoft Office™ software (Word™, Excel™, Power Point™), Internet, NCBI databases.
ECDL Licence.
Publications
Research project
GENETICS AND EPIGENETICS IN NEUROINFLAMMATION
This project will consider the role of oxidative stress involved in neurodegeneration and in the development of brain damage in multiple sclerosis. It will study the joint effects of protein Hsp70 (that is controlled by genes localised on the MHC class III region, on chromosome 6) and vitamin D (receptor encoded by a gene on chromosome 12) on the risk of developing the disease; risk defined by the interaction of genetic, environmental and epigenetic factors.
Multiple sclerosis (MS) is a multifactorial, disimmune disease, that affects the central nervous system and is characterised by an inflammatory process that leads to myelin damage. The pathogenesis of this disease is complex and involves different interconnected mechanisms. Furthermore different microRNAs, whose expression is altered in neurodegenerative disease, will be considered; the effects due to the action of pharmacological substances on patients, able to modify the expression levels of microRNAs, will be studied.
