Universitą degli studi di Pavia


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Manai's cv


Name and surname : Federico Manai
Adress: Via Manara Negrone 39, 27029 Vigevano (PV)
Date and place of birth: september 29, 1987, Abbiategrasso (MI)
Phone: -
E-mail: federico.manai01@universitadipavia.it

• November 2013-today: PhD student in Genetics, Molecular and Cellular Biology, University of Pavia (Italy). Laboratory of Functional Oncogenomics headed by Prof. Sergio Comincini.
• 2010-2012: Master degree in Experimental and Applied Biology, University of Pavia (Italy). Final evaluation: 110/110 cum laude. Title of thesis: “Ruolo della proteina Shc3 nella risposta allo stress metabolico nei glioblastomi” - Supervisor Prof. Lorenzo Magrassi, co-supervisor prof. Sergio Comincini.
• 2006-2010: Bachelor degree in Biological Sciences, University of Pavia (Italy). Final evaluation: 108/110. Title of thesis: “Analisi di sequenza di un aplogruppo mitocondriale raro in Italia” - Supervisor Prof. Antonio Torroni.

• September 2010 – June 2012: Traineeship in the laboratory of Neurosurgical Neurobiology headed by Prof. Lorenzo Magrassi – Institute of Molecular Genetics IGM-CNR, University of Pavia (Italy).
• 2009-2010: Traineeship in the laboratory of Genetics of Human Evolution headed by Prof. Antonio Torroni – Dipartement of Biology and Biotecnology, University of Pavia (Italy).

• Eukaryotic cell culture (tumoral stable and primary cell lines)
• Preparation of cellular fractions (i.e. nuclei extraction)
• Western Blot
• Immunoprecipitation with magnetic microbeads
• PCR, agarose gel electrophoresis and enzymatic digestion
• 2D-PAGE electrophoresis
• Transfections
• Cell viability assay and clonogenic assay
• Protein quantification
• Use of the optical microscope and fluorescence microscope
• Use of molecular databases

• Good command of bioinformatics tools (NCBI, Ensembl).
• Excellent knowledge of Microsoft Office™ tools (Word™, Excel™, PowerPoint™, Access™ and Publisher™).
• Basic knowledge of graphic design applications (Image J®, Photoshop®).
• Use of two operating systems (Window, Mac OS).
• Good command of internet browsers

• Italian: Mother tongue.
• English: Intermediate level.

• Convegno congiunto DGM-IGM
IGM-CNR of Pavia, February 22-23, 2011
Shc3 modulates glucose import and metabolism in high grade gliomas
Magrassi L, Azzalin A, Manai F
• XVI Congresso Nazionale e Corso Residenziale della Associazione Italiana di Neuro-Oncologia (AINO) e meeting congiunto AINO-ANOCEF (Association des Neuro-Oncologues d’Expression Franēaise Biology, diagnosis and treatment of Low Grade Glioma)
University of Milano, November 3-5, 2011
La reazione allo stress metabolico delle cellule di glioblastoma coltivate in vitro č mediata da parte della proteina Shc3
Magrassi L, Azzalin A, Pesantez Orellana H, Manai F, Ruggieri L

Research Project  

1) Role of the autophagic activation in the clearance of neurotoxic peptides in an in vitro model of Alzheimer’s disease
Alzheimer’s disease is a form of dementia caused by elevated level of 39 to 42 aminoacids β-amyloid peptides (Aβ) and their accumulation in neuritic plaques, neurofibrillary tangles, oxidative stress. The aim of my work is to study how and in what way the autophagy plays a role in counteracting the toxic effect of Aβ(1-40) and Aβ(1-42) peptides in an in vitro model of this disease.
2) Identification of circulating miRNA markers in pediatric patients affected by celiac disease
This project will be in collaboration with the Auxology Research Center of Pediatric Clinic, IRCCS Foundation Policlinico San Matteo of Pavia. The role of microRNA (miRNA) regulatory mechanisms of gene expression in celiac disease is little studied. It has been shown that miRNAs play an important role in regulating gene expression in many biological processes in physiological and pathological conditions including autoimmune disorders such as celiac disease. Furthermore, it has been described that miRNAs are important for the differentiation and function of the intestinal epithelium in mice. Today only one miRNA (miR449a) has been identified and is over-expressed pediatric patients with celiac disease. The purpose of this project is to conduct a pilot study on the expression of miRNAs in peripheral blood of pediatric patients with celiac disease and healthy subjects matched for age and sex, for a better understanding of the transcriptional regulation of genetic determinants involved in CD.


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