Universitą degli studi di Pavia
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Di Matteo's cv
Curriculum
CURRICULUM VITAE
ANNA DI MATTEO
Nata ad Avellino (AV), 06/07/1990
Cittadinanza: Italiana
Indirizzo di residenza: Ct.da Campoluongo,84 - Altavilla Silentina (SA)
Cell. +39 345 1534237
E-mail: anna.dimatteo01@ateneopv.it
Formal Education
2013-now Phd Program in “Genetica, Biologia Molecolare e Cellulare”
2011-13 Master’s degree in Molecular Biology and Genetics. Full marks and honours: 110/110 cum laude. University of Pavia.
2008–11
2003-2008 Bachelor degree (first level) in Molecular Biology. Full marks and honours: 110/110 cum laude. University of Pavia.
High school diploma (scientific studies). Full marks (100/100); Institute A. Gallotta, Eboli (SA).
Research Related Activities
2013-now PhD Program in “Genetics, Molecular and Cellular Biology”
2011-13 INTERNSHIP for Master’s Graduation Thesis
Institute of Molecular Genetics- National Research Council (IGM-CNR), Pavia, Italy
Supervisor: Dr. Claudia Ghigna Co-supervisors: Prof. Alessandra Montecucco, Dr. Serena Bonomi
Experimental thesis: HnRNP A1 regulates the alternative splicing of the Ron proto-oncogene and promotes the mesenchymal-to-epithelial transition program
My main project has been decipher the mechanism underlying aberrant splicing in cancer by using, as an experimental model, the scatter factor receptor and proto-oncogene Ron that accumulates during tumor progression of epithelial tissues and is able to confer an invasive phenotype to the expressing cells. In particular, I have focused on the molecular mechanism of splicing that produces ∆Ron isoform, a constitutively active variant of the receptor, studying the implication of two regulatory and antagonistic splicing factors, SRSF1 and hnRNP A1.
I acquired expertise in several molecular and cellular biology techniques: cloning techniques, plasmid preparation and purification, classical PCR, PCR-mediated mutagenesis, RT-PCR, electrophoresis on agarose gel, LM gel and acrylamide gel, gel extraction and purification. DNA, RNA and protein quantification. Eukaryotic cell culture. Eukaryotic cell transfection, DNA transfection, RNA extraction from cell pellet and purification, Western blotting.
2010–11
INTERNSHIP for First level Graduation Thesis
Institute of Molecular Genetics- National Research Council, Pavia, Italy
Supervisor: Dr. Claudia Ghigna; Co-supervisor: Prof. Guglielmina N. Ranzani
Experimental thesis: “RT-PCR analysis of alternative splicing of the Ron gene after over-expression of hnRNP A1 protein”.
Additional Education and professional experiences
2012 Theoretical course: “RNA Structure and Function” (Trieste, 27 February- 1 March 2012).
2012 Congress: 1st Post-EURASNET Symposium “Regulation of gene expression through RNA splicing” (Trieste, 24-27 March 2012).
2011–12
Part-time work at the Museum of Electrical Technology, University of Pavia. Roles: classification and organization of books in a library.
Competitions and adwards
2009-13 selected as alumnus of the “Collegio Nuovo-Fondazione Sandra e Enea Mattei”, historic college of Pavia and among the thirteen Italian university institutions of high cultural qualification set under the control of the Ministry of Education, Universities and Research (MIUR)
Languages
English: Good knowledge - Trinity College exam of spoken English, Grade 7 (2006)
Italian: mother tongue
Additional Skills and Qualifications:
Bioinformatics:
Good knowledge and familiarity with Microsoft Windows XP
Microsoft Office package
Adobe Photoshop
Bioinformatic tools: NCBI Pubmed, OMIM, GenBank, Map Viewer, Taxonomy, Entrez Nucleotide, Protein, BLAST.
References
If you require any further information or supporting materials please feel free to contact:
Dott. Claudia Ghigna
Section of Molecular and Cellular Biology of the Nucleus
Institute of Molecular Genetics - National Research Council,
Pavia, Italy
arneri@igm.cnr.it
telephone +39 0382 546324.
Research Project
Alternative splicing in the process of angiogenesis
Cancer cells induce new vessels formation from pre-existing ones. This process, named angiogenesis, allows the tumor to receive oxygen and nutrients for its growth. Alternative slicing is the process that allows the production of structurally and functionally different isoforms. Recently has emerged that several alternative splicing events occur only in cancer cells and a lot of these events give rise to new epitopes that can be used for immunotherapy. Thus, alternative splicing represents a powerful tool to identify new protein domains on the surface of endothelial cells useful to develop new anti-angiogenic strategies. One of our aims is to identify new isoforms of splicing on the surface of endothelial cells regulated during the process of angiogenesis in order to use them as therapeutic targets easily available to the drugs.
Publications
Publications
Bonomi S, di Matteo A, Buratti E, Cabianca DS, Baralle FE, Ghigna C*, Biamonti G*. 2013. HnRNP A1 controls a splicing regulatory circuit promoting mesenchymal-to-epithelial transition. Nucleic Acids Res. [Epub ahead of print]. *Corresponding author . IF: 8.278
Paronetto MP, Gallo S, di Matteo A, Ghigna C. Alternative pre-mRNA processing in cancer progression: clinical significance and therapeutic implications. Global Journal of Human Genetics & Gene Therapy. In preparazione.
Congress Abstracts and Presentations
Bonomi S, Di Matteo A, Buratti E, Baralle FE, Ghigna C, Biamonti G. 2013. “HnRNP A1 controls a splicing regulatory circuit promoting mesenchymal-to-epithelial transition (MET)”. SIBBM "Frontiers in Molecular Biology" Seminar 2013. Pavia, Italy. June 5-7, 2013. Poster.
Bonomi S, Di Matteo A, Buratti E, Baralle FE, Ghigna C, Biamonti G. 2013. “HnRNP A1 controls a splicing regulatory circuit promoting mesenchymal-to-epithelial transition (MET)”. Convegno congiunto IGM. Bologna, Italy. May 14, 2013. Oral communication.
Bonomi S, Di Matteo A, Buratti E, Baralle FE, Ghigna C, Biamonti G. 2012. “HnRNPA1 inhibits the production of ∆Ron isoform promoting mesenchymal to epithelial transition”. 1st Post-EURASNET Symposium. Trieste, Italy. March 24-27, 2012. Oral communication.
CURRICULUM VITAE
ANNA DI MATTEO
Nata ad Avellino (AV), 06/07/1990
Cittadinanza: Italiana
Indirizzo di residenza: Ct.da Campoluongo,84 - Altavilla Silentina (SA)
Cell. +39 345 1534237
E-mail: anna.dimatteo01@ateneopv.it
Formal Education
2013-now Phd Program in “Genetica, Biologia Molecolare e Cellulare”
2011-13 Master’s degree in Molecular Biology and Genetics. Full marks and honours: 110/110 cum laude. University of Pavia.
2008–11
2003-2008 Bachelor degree (first level) in Molecular Biology. Full marks and honours: 110/110 cum laude. University of Pavia.
High school diploma (scientific studies). Full marks (100/100); Institute A. Gallotta, Eboli (SA).
Research Related Activities
2013-now PhD Program in “Genetics, Molecular and Cellular Biology”
2011-13 INTERNSHIP for Master’s Graduation Thesis
Institute of Molecular Genetics- National Research Council (IGM-CNR), Pavia, Italy
Supervisor: Dr. Claudia Ghigna Co-supervisors: Prof. Alessandra Montecucco, Dr. Serena Bonomi
Experimental thesis: HnRNP A1 regulates the alternative splicing of the Ron proto-oncogene and promotes the mesenchymal-to-epithelial transition program
My main project has been decipher the mechanism underlying aberrant splicing in cancer by using, as an experimental model, the scatter factor receptor and proto-oncogene Ron that accumulates during tumor progression of epithelial tissues and is able to confer an invasive phenotype to the expressing cells. In particular, I have focused on the molecular mechanism of splicing that produces ∆Ron isoform, a constitutively active variant of the receptor, studying the implication of two regulatory and antagonistic splicing factors, SRSF1 and hnRNP A1.
I acquired expertise in several molecular and cellular biology techniques: cloning techniques, plasmid preparation and purification, classical PCR, PCR-mediated mutagenesis, RT-PCR, electrophoresis on agarose gel, LM gel and acrylamide gel, gel extraction and purification. DNA, RNA and protein quantification. Eukaryotic cell culture. Eukaryotic cell transfection, DNA transfection, RNA extraction from cell pellet and purification, Western blotting.
2010–11
INTERNSHIP for First level Graduation Thesis
Institute of Molecular Genetics- National Research Council, Pavia, Italy
Supervisor: Dr. Claudia Ghigna; Co-supervisor: Prof. Guglielmina N. Ranzani
Experimental thesis: “RT-PCR analysis of alternative splicing of the Ron gene after over-expression of hnRNP A1 protein”.
Additional Education and professional experiences
2012 Theoretical course: “RNA Structure and Function” (Trieste, 27 February- 1 March 2012).
2012 Congress: 1st Post-EURASNET Symposium “Regulation of gene expression through RNA splicing” (Trieste, 24-27 March 2012).
2011–12
Part-time work at the Museum of Electrical Technology, University of Pavia. Roles: classification and organization of books in a library.
Competitions and adwards
2009-13 selected as alumnus of the “Collegio Nuovo-Fondazione Sandra e Enea Mattei”, historic college of Pavia and among the thirteen Italian university institutions of high cultural qualification set under the control of the Ministry of Education, Universities and Research (MIUR)
Languages
English: Good knowledge - Trinity College exam of spoken English, Grade 7 (2006)
Italian: mother tongue
Additional Skills and Qualifications:
Bioinformatics:
Good knowledge and familiarity with Microsoft Windows XP
Microsoft Office package
Adobe Photoshop
Bioinformatic tools: NCBI Pubmed, OMIM, GenBank, Map Viewer, Taxonomy, Entrez Nucleotide, Protein, BLAST.
References
If you require any further information or supporting materials please feel free to contact:
Dott. Claudia Ghigna
Section of Molecular and Cellular Biology of the Nucleus
Institute of Molecular Genetics - National Research Council,
Pavia, Italy
arneri@igm.cnr.it
telephone +39 0382 546324.
Research Project
Alternative splicing in the process of angiogenesis
Cancer cells induce new vessels formation from pre-existing ones. This process, named angiogenesis, allows the tumor to receive oxygen and nutrients for its growth. Alternative slicing is the process that allows the production of structurally and functionally different isoforms. Recently has emerged that several alternative splicing events occur only in cancer cells and a lot of these events give rise to new epitopes that can be used for immunotherapy. Thus, alternative splicing represents a powerful tool to identify new protein domains on the surface of endothelial cells useful to develop new anti-angiogenic strategies. One of our aims is to identify new isoforms of splicing on the surface of endothelial cells regulated during the process of angiogenesis in order to use them as therapeutic targets easily available to the drugs.
Publications
Publications
Bonomi S, di Matteo A, Buratti E, Cabianca DS, Baralle FE, Ghigna C*, Biamonti G*. 2013. HnRNP A1 controls a splicing regulatory circuit promoting mesenchymal-to-epithelial transition. Nucleic Acids Res. [Epub ahead of print]. *Corresponding author . IF: 8.278
Paronetto MP, Gallo S, di Matteo A, Ghigna C. Alternative pre-mRNA processing in cancer progression: clinical significance and therapeutic implications. Global Journal of Human Genetics & Gene Therapy. In preparazione.
Congress Abstracts and Presentations
Bonomi S, Di Matteo A, Buratti E, Baralle FE, Ghigna C, Biamonti G. 2013. “HnRNP A1 controls a splicing regulatory circuit promoting mesenchymal-to-epithelial transition (MET)”. SIBBM "Frontiers in Molecular Biology" Seminar 2013. Pavia, Italy. June 5-7, 2013. Poster.
Bonomi S, Di Matteo A, Buratti E, Baralle FE, Ghigna C, Biamonti G. 2013. “HnRNP A1 controls a splicing regulatory circuit promoting mesenchymal-to-epithelial transition (MET)”. Convegno congiunto IGM. Bologna, Italy. May 14, 2013. Oral communication.
Bonomi S, Di Matteo A, Buratti E, Baralle FE, Ghigna C, Biamonti G. 2012. “HnRNPA1 inhibits the production of ∆Ron isoform promoting mesenchymal to epithelial transition”. 1st Post-EURASNET Symposium. Trieste, Italy. March 24-27, 2012. Oral communication.
