Universitą degli studi di Pavia


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Angeletti's curriculum


Personal Information
Surname Name: Angeletti Francesca
Date of Birth: 7 March 1987
E-mail: francesca.angeletti01@unipv.it
Adress: Cassine (AL)

Education and training
Dates: November/ December 2011
Admitted to the profession of Biologist, Section A (State examination of professional abilitation) – University of Genoa
Dates: October 2009 - 23 September 2011
Master degree in Medical-Pharmaceutical Biotechnologies with a score of 110/110 cum laude. Thesis:"Characterization of the role of chemokines SDF-1 and I-TAC in tumorigenesis of Glioblastoma”.- University of Genoa
Dates: October 2006 - 25 September 2009
Bachelor degree in Biotechnology with the score of 110/110 cum laude. Thesis " Toxic effect of fractions derived from olive mill wastewater (OMW) with respect to microbial flora isolated from environmental and food matrices and strains of collection." – University of Genoa
Dates: 15 September 2001 - 01 July 2006
Scientific biological high school leaving qualifications with a score of 100/100.- ITIS Carlo Barletti, Acqui Terme.
Work experience
Dates: 01 november 2012 → now
PhD program in Genetics, cellular and molecular Biology; University of Pavia.
Study of the role of different microRNA in the autophagic process in Glioblastoma’s cells and in vitro models of Alzheimer desease.
Supervisor: Prof.Sergio Comincini.
Dates: 13 june 2012 - 30 november 2012
Pharmaceutical informant- Green Pharma Srl
Dates: october 2009 - september 2011
Student-Trainee; I worked with the laboratory staff for the compilation of my experimental thesis. I studied the role oftwo chemokines: SDF-1 and I-TAC in the development of glioblastoma. For this study I used an in

vitro model representative of the subpopulation of glioblastoma's cancer stem cells.- Pharmacology Laboratory c.o. University of Genoa
Dates: september 2008 - september 2009
Student- traineeI worked with the laboratory staff for the compilation of my experimental thesis. During the stage I analyzed the potential antimicrobial effect of a pollutant olive oil production by-product : the olive mill wastewater. The study has been made testing the effect of this product on Gram+ and Gram- bacteria coming from natural matrices (dietary and environmental) and collection.- Analisi & Controlli S.p.a Largo Rosanna Benzi 10 (CBA), 16132 Genova (Italia).

Mother tongue: Italian
Secon language: English
Technical skills and competences
Laboratory techniques acquired:
- Cellular Biology: Cell lines culture. Isolation and primary culture of cells derived from post-surgical cancer tissue. Isolation of neural stem cells and human Glioblastoma cancer stem cells. Culture of cancer stem cells in monolayer, spherogenesis and differentiation assays. Proliferation and cellular vitality (cytotoxicity test: MTT)
- Molecular Biology: extraction and purification of nucleic acids from in vitro cultures of cells. Electrophoresis in agarose gel. RT-PCR (retrotranscriptase), PCR reactions.
- Biochemistry: extraction of proteins and following dosage (Bradford technique), separation of proteins by SDS PAGE and Western Blot.
- Immunofluorescence on adhering cells and neurospheres.
- Microbiology: isolation of bacterial strains from food, water and fuel. Maintenance of bacterial strains on slant agar. Evaluation of antimicrobial activity of different substances.
Computer skills and competences
PC Operative systems: Windows; good command of Office tools (Word, Excel and Power Point); good command of NCBI database (www.ncbi.nlm.nih.gov).


  • Combined EGFR and Autophagy Modulation Impairs Cell Migration and Enhances Radiosensitivity in Human Glioblastoma Cells. Palumbo S, Tini P, Toscano M, Allavena G, Angeletti F, Manai F, Miracco C, Comincini S, Pirtoli L. (J.Cell Physiol., 2014); 229 :1863-1873.
  • Autophagy Is Modulated in Human Neuroblastoma Cells Through Direct Exposition to Low Frequency Electromagnetic Fields. Marchesi N, Osera C, Fassina L, Amadio M, Angeletti F, Morini M, Magenes G, Venturini L, Biggiogera M, Ricevuti G, Govoni S, Caorsi S, Pascale A, Comincini S. (J.Cell Physiol., 2014); 229: 1776-1786.
  • microRNA-17 regulates the expression of ATG7 and modulates the autophagy process, improving the sensitivity to temozolomide and low-dose ionizing radiation treatments in human glioblastoma cells. Comincini S, Allavena G, Palumbo S, Morini M, Durando F, Angeletti F, Pirtoli L, Miracco C. (Cancer Biol. Ther, 2013); 4: 574-86
  • Somatostatin receptor 1, 2 and 5 activation leads to C6 glioma growth arrest in vitro and in vivo; analysis of the intracellular pathways involved. Gatti M, Pattarozzi A, Würth R, Angeletti F, Barbieri F, Florio T (Bollettino della Societą italiana di biologia sperimentale, 2011); 84:139-140.

Research project

Development of preclinical experimental protocols to induce human tumor cell death

Cancer of the central nervous system (CNS) is a dramatic health emergency. Despite decades of research on tumor biology, some cancers are still characterized by a dismal prognosis, particularly those overwhelmingly resistant to conventional radio- and chemo-therapeutic treatments. Therefore, novel therapies aimed to induce neoplastic cell death are clearly needed to improve current treatments. During my PhD course I’ll therefore interested in the development of preclinical experimental anticancer protocols: the molecular strategy used is to induce programmed cell death processes of autophagic type directly into the tumor cells, mainly related to astrocytic tumors, known to be highly malignant. To this purpose, cellular models representing human astrocytic tumors are largely employed. The practical interest is therefore directed to the development of adjuvant therapy compared with existing protocols based on radio and chemotherapy and development of new potential anticancer drug and molecular-based compounds. In particular, molecular components based on DNA, RNA or proteins that interfere at the molecular level with the proliferative capacity of cancer cells by inducing programmed cell death processes are designed and assayed using normal and tumor in vitro systems.
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