Università degli studi di Pavia
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Badiale's curriculum
CURRICULUM VITAE: CLAUDIA BADIALE
PERSONAL INFORMATION:
Address(es):
• Via Padre Lorenzo Piazza 64/31 17019 Varazze (SV)
• Dipartimento di Biologia e Biotecnologie “Buzzati Traverso” -University of Pavia
Via Ferrata 9, 27100 PAVIA
Tel: +39-0382-985541
Fax: +39-0382-528496
Italy
Telephone(s): 347-370 40 60
E-mail: claudia.bady@gmail.com
Nationality: Italian
Date of birth: 07 October 1988
Gender: female
OCCUPATIONAL FIELD: Molecular Biology
PhD student at the Laboratory of Molecular and Cellular Biology
(Department of Biology and Biotechnology “Buzzati-Traverso”- University of Pavia)
WORK EXPERIENCE:
• 2014: Tutor for the Molecular Biology and Genetics Master course at the University of Pavia.
• 2014: 3 months internship at the “Universitat Autonoma of Barcelona”, in the laboratory of Professor Herrera A.R. working on horse meiosis.
• 2012: Experimental Thesis Internship “Inter-individual variation of a mammalian centromere”.
• 2010: Experimental Thesis Internship “Instabilità del genoma in cellule umane con aumentata espressione di geni coinvolti nel trasporto vescicolare” (genomic instability in human cells with increased expression of genes involved in vesicular transport.)
• 2010: Tutor for III years Molecular Biology students in the Laboratory of Experimental Biology at the University of Pavia.
ACADEMIC AND PROFESSIONAL FORMATION:
2012 Tutor for students, laboratory of Molecular Biology. University of Pavia.
2010-2012 Master Degree in Molecular Biology and Genetics (110/110 cum laude), Laboratory of Molecular and Cellular Biology, Department of Genetics and Microbiology – University of Pavia.
2010 Tutor for students, laboratory of Molecular biology. University of Pavia.
2007-2010 Degree in Biological Sciences (109/110) (Italian degree similar to bachelor’s degree), Laboratory of Molecular and Cellular Biology, Department of Genetics and Microbiology – University of Pavia.
2002-2007 Linguistic High School graduation qualification (97/100), Liceo Ginnasio Statale "Gabriello Chiabrera" Savona, Via Caboto, 2 .17100 Savona
PERSONAL SKILLS AND COMPETENCES:
In vitro cultures of mammalian cells
Standard chromosomal preparations
PNA-FISH
DNA extraction and purification
ChIP
PCR and Real Time-PCR
Protein extraction
Use of laboratory equipment like autoclave, electrophoretic devices, microscopes, thermostats, etc.
MOTHER LANGUAGE: Italian
English: High-intermediate
French: Base
Spanish: intermediate (D.B.E. B2 level)
INFORMATIC SKILLS
Office, Open-Office and Mac. On-line databases consultation. Adobe Photoshop, GraphPad, MicroMeasure.
ORGANIZATIONAL COMPETENCES
• Production and analysis of experimental data
• Formal presentation of the results
• Collect data from literature
• Tutoring activity
• Routine activities in the lab
PUBLICATIONS
MEETING ABSTRACTS
The sliding behaviour of horse chromosome 11 centromere
(XIII FISV Congress- Pisa, 2014)
A.Mazzagatti, FM. Piras, SG. Nergadze, E. Belloni, F. Cerutti, C. Badiale, E. Giulotto, E. Raimondi
Physical organization and CENP binding ability of horse centromeric satellite DNA families
(19th International Chromosome Conference – Bologna, 2013)
Raimondi E, Belloni E, Piras FM, Mazzagatti A, Badiale C, Meinardi B, Castro Duque AF, Savini G, Cerutti F, Bensi M, Nergadze SG, Giulotto E.
Analysis of the physical organization and of the CENP binding ability of horse centromeric satellite DNA families
(Convegno AGI, Cortona. Settembre 2013)
Elisa Belloni, Francesca Piras, Alice Mazzagatti, Claudia Badiale, Benedetta Meinardi, Andres Castro, Grazia Savini, Federico Cerutti, Mirella Bensi, Solomon Nergadze, Elena Raimondi and Elena Giulotto.
Different mechanisms contribute to the rapid genome evolution of Equids
(International Wild Equid Conference, Vienna. September 2012)
Elena Giulotto, Francesca M Piras, Francesco Vella, Claudia Badiale, Elisa Belloni, Federico Cerutti, Riccardo Gamba, Paola Pellanda, Valerio Vitelli, Oliver A. Ryder, Elena Raimondi, Solomon G Nergadze.
RESEARCH ARTICLES
Centromere Sliding on a Mammalian Chromosome. Purgato S, Belloni E, Piras MF, Zoli M, Badiale C, Cerutti F, Mazzagatti A, Perini G, Della Valle G, Nergadze SG, Sullivan KF, Raimondi E, Rocchi M, Giulotto E. Chromosoma DOI 10.1007/s00412-014-0493-6 (2014).
RESEARCH PROJECT
Characterization of the centromeric region in Equids’ Neocentromeres
In previous works from our laboratory (Wade et al. 2009) two domains of binding of the protein CENP-A were described in the centromeric region of the horse chromosome 11 (ECA11). Those studies demonstrated that the centromere of the horse chromosome 11 is an Evolutionary Neocentromere (ENC) stable and fixed in the species. This ENC is characterized by the absence of satellite DNA, tipically present at mammalian centromeres that prevent their molecular characterization.
Taking advantage of this particular characteristic, during my Master Degree I contributed to the study of the centromeric region and CENP-A binding domains in other five individuals using ChIP coupled to RT-PCR analysis and Sequencing of the obtained fragments.
During my PhD I will concentrate on the study of centromeric regions in other Equus species such as E. asinus, E. grevy and E. burchelli that, as previously demonstrated (Piras et al. 2010), possess several centromeres devoid of satellite DNA detectable by standard in situ fluorescence techniques.
PERSONAL INFORMATION:
Address(es):
• Via Padre Lorenzo Piazza 64/31 17019 Varazze (SV)
• Dipartimento di Biologia e Biotecnologie “Buzzati Traverso” -University of Pavia
Via Ferrata 9, 27100 PAVIA
Tel: +39-0382-985541
Fax: +39-0382-528496
Italy
Telephone(s): 347-370 40 60
E-mail: claudia.bady@gmail.com
Nationality: Italian
Date of birth: 07 October 1988
Gender: female
OCCUPATIONAL FIELD: Molecular Biology
PhD student at the Laboratory of Molecular and Cellular Biology
(Department of Biology and Biotechnology “Buzzati-Traverso”- University of Pavia)
WORK EXPERIENCE:
• 2014: Tutor for the Molecular Biology and Genetics Master course at the University of Pavia.
• 2014: 3 months internship at the “Universitat Autonoma of Barcelona”, in the laboratory of Professor Herrera A.R. working on horse meiosis.
• 2012: Experimental Thesis Internship “Inter-individual variation of a mammalian centromere”.
• 2010: Experimental Thesis Internship “Instabilità del genoma in cellule umane con aumentata espressione di geni coinvolti nel trasporto vescicolare” (genomic instability in human cells with increased expression of genes involved in vesicular transport.)
• 2010: Tutor for III years Molecular Biology students in the Laboratory of Experimental Biology at the University of Pavia.
ACADEMIC AND PROFESSIONAL FORMATION:
2012 Tutor for students, laboratory of Molecular Biology. University of Pavia.
2010-2012 Master Degree in Molecular Biology and Genetics (110/110 cum laude), Laboratory of Molecular and Cellular Biology, Department of Genetics and Microbiology – University of Pavia.
2010 Tutor for students, laboratory of Molecular biology. University of Pavia.
2007-2010 Degree in Biological Sciences (109/110) (Italian degree similar to bachelor’s degree), Laboratory of Molecular and Cellular Biology, Department of Genetics and Microbiology – University of Pavia.
2002-2007 Linguistic High School graduation qualification (97/100), Liceo Ginnasio Statale "Gabriello Chiabrera" Savona, Via Caboto, 2 .17100 Savona
PERSONAL SKILLS AND COMPETENCES:
In vitro cultures of mammalian cells
Standard chromosomal preparations
PNA-FISH
DNA extraction and purification
ChIP
PCR and Real Time-PCR
Protein extraction
Use of laboratory equipment like autoclave, electrophoretic devices, microscopes, thermostats, etc.
MOTHER LANGUAGE: Italian
English: High-intermediate
French: Base
Spanish: intermediate (D.B.E. B2 level)
INFORMATIC SKILLS
Office, Open-Office and Mac. On-line databases consultation. Adobe Photoshop, GraphPad, MicroMeasure.
ORGANIZATIONAL COMPETENCES
• Production and analysis of experimental data
• Formal presentation of the results
• Collect data from literature
• Tutoring activity
• Routine activities in the lab
PUBLICATIONS
MEETING ABSTRACTS
The sliding behaviour of horse chromosome 11 centromere
(XIII FISV Congress- Pisa, 2014)
A.Mazzagatti, FM. Piras, SG. Nergadze, E. Belloni, F. Cerutti, C. Badiale, E. Giulotto, E. Raimondi
Physical organization and CENP binding ability of horse centromeric satellite DNA families
(19th International Chromosome Conference – Bologna, 2013)
Raimondi E, Belloni E, Piras FM, Mazzagatti A, Badiale C, Meinardi B, Castro Duque AF, Savini G, Cerutti F, Bensi M, Nergadze SG, Giulotto E.
Analysis of the physical organization and of the CENP binding ability of horse centromeric satellite DNA families
(Convegno AGI, Cortona. Settembre 2013)
Elisa Belloni, Francesca Piras, Alice Mazzagatti, Claudia Badiale, Benedetta Meinardi, Andres Castro, Grazia Savini, Federico Cerutti, Mirella Bensi, Solomon Nergadze, Elena Raimondi and Elena Giulotto.
Different mechanisms contribute to the rapid genome evolution of Equids
(International Wild Equid Conference, Vienna. September 2012)
Elena Giulotto, Francesca M Piras, Francesco Vella, Claudia Badiale, Elisa Belloni, Federico Cerutti, Riccardo Gamba, Paola Pellanda, Valerio Vitelli, Oliver A. Ryder, Elena Raimondi, Solomon G Nergadze.
RESEARCH ARTICLES
Centromere Sliding on a Mammalian Chromosome. Purgato S, Belloni E, Piras MF, Zoli M, Badiale C, Cerutti F, Mazzagatti A, Perini G, Della Valle G, Nergadze SG, Sullivan KF, Raimondi E, Rocchi M, Giulotto E. Chromosoma DOI 10.1007/s00412-014-0493-6 (2014).
RESEARCH PROJECT
Characterization of the centromeric region in Equids’ Neocentromeres
In previous works from our laboratory (Wade et al. 2009) two domains of binding of the protein CENP-A were described in the centromeric region of the horse chromosome 11 (ECA11). Those studies demonstrated that the centromere of the horse chromosome 11 is an Evolutionary Neocentromere (ENC) stable and fixed in the species. This ENC is characterized by the absence of satellite DNA, tipically present at mammalian centromeres that prevent their molecular characterization.
Taking advantage of this particular characteristic, during my Master Degree I contributed to the study of the centromeric region and CENP-A binding domains in other five individuals using ChIP coupled to RT-PCR analysis and Sequencing of the obtained fragments.
During my PhD I will concentrate on the study of centromeric regions in other Equus species such as E. asinus, E. grevy and E. burchelli that, as previously demonstrated (Piras et al. 2010), possess several centromeres devoid of satellite DNA detectable by standard in situ fluorescence techniques.
