Universitą degli studi di Pavia
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Rovelli's cv
CURRICULUM
PERSONAL INFORMATION:
Name and surname: Cristina Rovelli
Address: Via Mirabello 113, 27100 Pavia
Date and place of birth: November 28th 1987, Pavia
Phone number: +393386228161
EDUCATION AND TRAINING
Current position: Ph.D student in Genetics Molecular and Cellular Biology - Doctoral School in Life Sciences “Camillo Golgi”- University of Pavia
2009-2011: Postgraduate degree in Experimental and Applied Biology, University of Pavia; final evaluation: 110/110 cum laude. Title of the thesis:”Effects of Borophenylalanine-fructose, a radiosensitizing agent for Boron Neutron Capture Therapy (BNCT), on human Astrocitoma cells”. Supervisor: Prof. O. Pastoris
2006-2009: Bachelor’s degree in Biology, University of Pavia; Title of the thesis: “Inreactions between European hare (Lepus europaeus) and Cottontail (Sylvilagus floridanus) during the feeding activity”. Supervisor: Prof. Prigioni
2001-2006: Scientific lyceum “N. Copernico” - Pavia
TECHNICAL SKILLS
Eukaryotic cell cultures, Toxicological analysis techniques as MTT assay, Clonogenic assay and Live/Dead test with Calcein and propidium iodide colouration, bioinformatics competences: use of the main genomic databases.
INFORMATIC SKILLS
Knowledge of Windows, linux sistems such as Ubuntu and Osx. Office package (Word, Excel, Power Point), Latex base through the use of lyx. Use of Browser for internet browsing.
LINGUISTIC SKILLS
English
RESEARCH PROJECT
Effectiveness and radiotoxicity of BNCT in animal model
The PhD project is linked with the Boron Neutron Capture Therapy (BNCT), a non conventional and experimental radiotherapy for treating locally invasive malignant tumors. It is a two step procedure: first, the patient is injected with a tumor localizing drug containing Boro 10 (10B) that has a high propensity or cross section (σ) to capture neutrons. In the second step, the patient is radiated with neutron which interact with the capture agent thereby resulting in a biologically destructive nuclear caption reaction: 10B(n,α)7Li.
The BNCT success is due to the major backlog of the 10B in the tumor cells rather than in the healthy cells. The selective absorption is due to the use of boron compound (carrier) as Borophenylalanine - fructose that can enter in the cell with a active transport mechanism that involves LAT-1; For that reason BPA-f is the compound most commonly used in clinical.
In particular the PhD project wants to text the effectiveness and the radiotoxicity of BNCT in an animal model, such as rat with lung metastases.
The first tests will be carried out with BPA-f, this drug will be injected in animals with tumor; after a few ours of infusion with BPA-f the animals will be exposed to neutron irradiation in the Triga Mark II LENA reactor; once irradiated, the animals will be sacrificed and their exposed organs will be analyzed to asses the effects of BNCT on lung metastases.
PERSONAL INFORMATION:
Name and surname: Cristina Rovelli
Address: Via Mirabello 113, 27100 Pavia
Date and place of birth: November 28th 1987, Pavia
Phone number: +393386228161
EDUCATION AND TRAINING
Current position: Ph.D student in Genetics Molecular and Cellular Biology - Doctoral School in Life Sciences “Camillo Golgi”- University of Pavia
2009-2011: Postgraduate degree in Experimental and Applied Biology, University of Pavia; final evaluation: 110/110 cum laude. Title of the thesis:”Effects of Borophenylalanine-fructose, a radiosensitizing agent for Boron Neutron Capture Therapy (BNCT), on human Astrocitoma cells”. Supervisor: Prof. O. Pastoris
2006-2009: Bachelor’s degree in Biology, University of Pavia; Title of the thesis: “Inreactions between European hare (Lepus europaeus) and Cottontail (Sylvilagus floridanus) during the feeding activity”. Supervisor: Prof. Prigioni
2001-2006: Scientific lyceum “N. Copernico” - Pavia
TECHNICAL SKILLS
Eukaryotic cell cultures, Toxicological analysis techniques as MTT assay, Clonogenic assay and Live/Dead test with Calcein and propidium iodide colouration, bioinformatics competences: use of the main genomic databases.
INFORMATIC SKILLS
Knowledge of Windows, linux sistems such as Ubuntu and Osx. Office package (Word, Excel, Power Point), Latex base through the use of lyx. Use of Browser for internet browsing.
LINGUISTIC SKILLS
English
RESEARCH PROJECT
Effectiveness and radiotoxicity of BNCT in animal model
The PhD project is linked with the Boron Neutron Capture Therapy (BNCT), a non conventional and experimental radiotherapy for treating locally invasive malignant tumors. It is a two step procedure: first, the patient is injected with a tumor localizing drug containing Boro 10 (10B) that has a high propensity or cross section (σ) to capture neutrons. In the second step, the patient is radiated with neutron which interact with the capture agent thereby resulting in a biologically destructive nuclear caption reaction: 10B(n,α)7Li.
The BNCT success is due to the major backlog of the 10B in the tumor cells rather than in the healthy cells. The selective absorption is due to the use of boron compound (carrier) as Borophenylalanine - fructose that can enter in the cell with a active transport mechanism that involves LAT-1; For that reason BPA-f is the compound most commonly used in clinical.
In particular the PhD project wants to text the effectiveness and the radiotoxicity of BNCT in an animal model, such as rat with lung metastases.
The first tests will be carried out with BPA-f, this drug will be injected in animals with tumor; after a few ours of infusion with BPA-f the animals will be exposed to neutron irradiation in the Triga Mark II LENA reactor; once irradiated, the animals will be sacrificed and their exposed organs will be analyzed to asses the effects of BNCT on lung metastases.
