Università degli studi di Pavia
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Grignani's CV
Personal Details
Name: Pierangela Grignani
Date of Birth: Pavia, 20 January 1972
Nationality: Italian
e-mail pierangela.grignani@unipv.it
laboratorio: 0382-987810
Academic and Professional Qualifications
November 2010: Student of “Pathology and Medical Genetics” Ph.D program (XXVIciclo) - University of Pavia.
July 2004: Postgraduate Specialisation in Clinical Biochemistry ( 3rd level university degree).- School of Specialisation in Clinical Biochemistry, Faculty of Medicine and Surgery -University of Pavia, Italy.
November 1999: Biologist Professional State Examination .
September 1998: Second level Biological Sciences degree (2nd level university degree)- Faculty of Science - University of Pavia- Italy.
Professional experiences
July 2004 / April 2012
Research Grant made available by University of Pavia (Bando Prot. 6730 e 6731/27 January 2004). The research ”Caratterizzazione dei polimorfismi del DNA in ambito genetico-forense”(DNA polymorphisms characterization in the field of forensic-genetics research) was conducted in the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
April 2001 / March 2004
Research fellowship made available by IRCCS Policlinico “San Matteo”, Pavia - Italy (Bando Prot. 34239/2000/Gen). The research “Biologia molecolare applicata al trapianto di midollo osseo: monitoraggio quantitativo del chimerismo”(Molecular biology applied to bone marrow transplantation: chimerism follow-up by a quantitative approach) was conducted in the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
Jenuary 1999 / December 2000
Research fellowship made available by IRCCS Policlinico “San Matteo” (Bando Prot. 3180/gen/98). The research “Analisi molecolare del chimerismo e della recidiva nel trapianto di midollo osseo in pazienti pediatrici”(Chimerism and relapse molecular analysis in children given bone marrow transplantation) was conducted in the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy
October 1998/ November 1999: Training at the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
October 1998 – present
Continuous collaboration in the research filed of identification and paternity tests at the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
Expertise in:
ü Samples characterization by generic and specific diagnosis for blood, sperm, saliva human fluids.
ü DNA purification from the following samples: whole blood, buffy coat, dried blood spots, body fluids, buccal swabs, saliva, sperm, compact bone, skeletal remains, teeth, tissue, paraffin-embedded tissue, fixed tissues, nails, hair follicles and hair shafts.
ü DNA quantification by RealTime PCR.
ü DNA amplification by PCR and multiplex-PCR.
ü DNA polymorphisms characterization VNTRs (variable number of tandem repeats), STRs (short tandem repeats) and SNPs (single nucleotide polymorphisms) by PCR, Single Base Extension, and electrophoresis methods.
ü mtDNA sequencing analysis and mitochondrial haplogroups analysis
ü Electrophoresis methods: agarose gel, PAGE, capillary electrophoresis on ABI PRISM 310 Genetic Analyzer.
ü RFLPs characterization by Southern blotting analysis
ü RNA isolation.
ü mRNA to cDNA reverse transcription (RT-PCR)
ü Detection of Minimal Residual Desease (MRD) in acute leukemia by RT-PCR analysis of fusion gene transcripts (bcr-abl and pml-rarα).
ü Donor/recipient mixed chimerism analysis by STRs in children given a bone marrow transplant(BMT).
ü ABI PRISM 310 Genetic Analyzer instruments.
ü GeneScan Software, Genotyper Software and GeneMapper Software analysis.
ü Populations studies to determine STRs and SNPs alleles frequencies.
ü Biostatistics analysis in paternity and identification tests.
Foreign Languages
English: B1 level
French: elementary level.
Informatics skills
Operating systems: Windows, Macintosh, Vista
Software MS Office
Specific Software GeneScan Analysis Software
Genotyper Software
GeneMapper Software
Name: Pierangela Grignani
Date of Birth: Pavia, 20 January 1972
Nationality: Italian
e-mail pierangela.grignani@unipv.it
laboratorio: 0382-987810
Academic and Professional Qualifications
November 2010: Student of “Pathology and Medical Genetics” Ph.D program (XXVIciclo) - University of Pavia.
July 2004: Postgraduate Specialisation in Clinical Biochemistry ( 3rd level university degree).- School of Specialisation in Clinical Biochemistry, Faculty of Medicine and Surgery -University of Pavia, Italy.
November 1999: Biologist Professional State Examination .
September 1998: Second level Biological Sciences degree (2nd level university degree)- Faculty of Science - University of Pavia- Italy.
Professional experiences
July 2004 / April 2012
Research Grant made available by University of Pavia (Bando Prot. 6730 e 6731/27 January 2004). The research ”Caratterizzazione dei polimorfismi del DNA in ambito genetico-forense”(DNA polymorphisms characterization in the field of forensic-genetics research) was conducted in the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
April 2001 / March 2004
Research fellowship made available by IRCCS Policlinico “San Matteo”, Pavia - Italy (Bando Prot. 34239/2000/Gen). The research “Biologia molecolare applicata al trapianto di midollo osseo: monitoraggio quantitativo del chimerismo”(Molecular biology applied to bone marrow transplantation: chimerism follow-up by a quantitative approach) was conducted in the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
Jenuary 1999 / December 2000
Research fellowship made available by IRCCS Policlinico “San Matteo” (Bando Prot. 3180/gen/98). The research “Analisi molecolare del chimerismo e della recidiva nel trapianto di midollo osseo in pazienti pediatrici”(Chimerism and relapse molecular analysis in children given bone marrow transplantation) was conducted in the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy
October 1998/ November 1999: Training at the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
October 1998 – present
Continuous collaboration in the research filed of identification and paternity tests at the Department of Legal Medicine, Forensic and Pharmaco-Toxicological Sciences - Forensic Genetics Laboratory, University of Pavia, Italy.
Expertise in:
ü Samples characterization by generic and specific diagnosis for blood, sperm, saliva human fluids.
ü DNA purification from the following samples: whole blood, buffy coat, dried blood spots, body fluids, buccal swabs, saliva, sperm, compact bone, skeletal remains, teeth, tissue, paraffin-embedded tissue, fixed tissues, nails, hair follicles and hair shafts.
ü DNA quantification by RealTime PCR.
ü DNA amplification by PCR and multiplex-PCR.
ü DNA polymorphisms characterization VNTRs (variable number of tandem repeats), STRs (short tandem repeats) and SNPs (single nucleotide polymorphisms) by PCR, Single Base Extension, and electrophoresis methods.
ü mtDNA sequencing analysis and mitochondrial haplogroups analysis
ü Electrophoresis methods: agarose gel, PAGE, capillary electrophoresis on ABI PRISM 310 Genetic Analyzer.
ü RFLPs characterization by Southern blotting analysis
ü RNA isolation.
ü mRNA to cDNA reverse transcription (RT-PCR)
ü Detection of Minimal Residual Desease (MRD) in acute leukemia by RT-PCR analysis of fusion gene transcripts (bcr-abl and pml-rarα).
ü Donor/recipient mixed chimerism analysis by STRs in children given a bone marrow transplant(BMT).
ü ABI PRISM 310 Genetic Analyzer instruments.
ü GeneScan Software, Genotyper Software and GeneMapper Software analysis.
ü Populations studies to determine STRs and SNPs alleles frequencies.
ü Biostatistics analysis in paternity and identification tests.
Foreign Languages
English: B1 level
French: elementary level.
Informatics skills
Operating systems: Windows, Macintosh, Vista
Software MS Office
Specific Software GeneScan Analysis Software
Genotyper Software
GeneMapper Software
