Universitą degli studi di Pavia
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Incandela's CV
Name: Maria Loreto Incandela
Date and place of birth: 07 May 1981, Palermo
Address: Via Letto Santo snc, 98077, S.Stefano di Camastra (ME)
Laboratory telephone number: +39 0382 985579
E-mail: mariella.incandela@gmail.com, marialoreto.incandela@unipv.it
EDUCATION
2010-present PhD in Genetic and Biomolecular Sciences, PhD Program in Life Sciences "Camillo Golgi", University of Pavia, XXVI cycle (2010/2013).
Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Dr. Maria Rosalia Pasca).
2010 Admitted to the profession of biologist at the University of Pavia.
2010 Degree in Experimental and Applied Biology, Class 6 / S (DM509/99), curriculum Biomolecular Sciences and Genetics at University of Pavia.
Thesis title: "Molecular characterization of mutants resistant to Benzothiazinones in Mycobacterium smegmatis”.
Supervisor: Dr. Maria Rosalia Pasca
Final vote: 110/110 cum laude
2008 Degree in Biology, Class 12 (DM509/99), curriculum Biosanitarium at University of Palermo.
Thesis title: "Characterization of the mutant strain ΔhisX2 of Streptomyces coelicolor M145 A3(2)”.
Supervisor: Prof. Anna Maria Puglia
Final vote: 104/110
2000 Classical studies at Liceo "A. Manzoni, "Mistretta (ME)
Final vote 93/100
PROFESSIONAL EXPERIENCE
2011-present Fellowship for collaboration in research (“Assegno per collaborazione ad attivitą di ricerca”), "Development of a new diagnostic kit for the identification of infections caused by mycobacteria "; Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Prof. Giovanna Riccardi; funded by Sentinel CH SpA).
2010-2011 Fellowship for research, "Using the gene Rv3790 of Mycobacterium tuberculosis for diagnostic purposes ""; Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Prof. Giovanna Riccardi).
2009-2010 Thesis internship at the Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Dr. Maria Rosalia Pasca).
2008 Thesis internship at the Laboratory of Molecular Microbiology, Department of Cell and Developmental Biology, Faculty of Sciences MM.FF.NN, University of Palermo, (Supervisor: Prof. Anna Maria Puglia).
TECHNICAL SKILLS
Microbiological techniques: Preparation of solid and liquid culture media; techniques of sterilization (autoclave, oven, UV); preparation of bacterial cultures (E. coli, S. coelicolor, M. smegmatis); methods for determining the antimicrobial activity of different antibiotics; preparation of competent cells and transformation of E. coli and M. smegmatis using electroporation.
Molecular biology techniques: extraction of plasmidic DNA; extraction of genomic DNA from enteric bacteria and mycobacteria; extraction of total RNA from mycobacteria; DNA digestion with restriction enzymes; cloning techniques; PCR amplification; Southern blotting; Redirect-technology; construction of a genomic library; Retrotranscription-PCR, Real-Time PCR using: intercalating dyes (e.g. SYBR Green) and probes specific for a fragment (e.g. Dual-labeled probes, TaqMan assay).
Bioinformatics techniques: molecular phylogenetic analysis within the genus Mycobacterium, multiple sequence alignment, identification of orthologous genes and evaluation of the degree of homology.
Bioinformatic analysis using the following programs or sites: Eagle view, ClustalW, EXPASY proteomics server, MycoBrowser, SIB Microbe Browser, Sanger istitute mycobacterium blast server.
Good knowledge of the software:
Microsoft Windows XP/Vista, Microsoft Office 2007 Word/Excel/PowerPoint/Publisher.
Knowledge of various graphic programs: Adobe Photoshop, Corel PaintShop Photo.
FOREIGN LANGUAGES
English good knowledge
French basic knowledge
Date and place of birth: 07 May 1981, Palermo
Address: Via Letto Santo snc, 98077, S.Stefano di Camastra (ME)
Laboratory telephone number: +39 0382 985579
E-mail: mariella.incandela@gmail.com, marialoreto.incandela@unipv.it
EDUCATION
2010-present PhD in Genetic and Biomolecular Sciences, PhD Program in Life Sciences "Camillo Golgi", University of Pavia, XXVI cycle (2010/2013).
Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Dr. Maria Rosalia Pasca).
2010 Admitted to the profession of biologist at the University of Pavia.
2010 Degree in Experimental and Applied Biology, Class 6 / S (DM509/99), curriculum Biomolecular Sciences and Genetics at University of Pavia.
Thesis title: "Molecular characterization of mutants resistant to Benzothiazinones in Mycobacterium smegmatis”.
Supervisor: Dr. Maria Rosalia Pasca
Final vote: 110/110 cum laude
2008 Degree in Biology, Class 12 (DM509/99), curriculum Biosanitarium at University of Palermo.
Thesis title: "Characterization of the mutant strain ΔhisX2 of Streptomyces coelicolor M145 A3(2)”.
Supervisor: Prof. Anna Maria Puglia
Final vote: 104/110
2000 Classical studies at Liceo "A. Manzoni, "Mistretta (ME)
Final vote 93/100
PROFESSIONAL EXPERIENCE
2011-present Fellowship for collaboration in research (“Assegno per collaborazione ad attivitą di ricerca”), "Development of a new diagnostic kit for the identification of infections caused by mycobacteria "; Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Prof. Giovanna Riccardi; funded by Sentinel CH SpA).
2010-2011 Fellowship for research, "Using the gene Rv3790 of Mycobacterium tuberculosis for diagnostic purposes ""; Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Prof. Giovanna Riccardi).
2009-2010 Thesis internship at the Laboratory of Molecular Microbiology, Department of Genetics and Microbiology "Adriano Buzzati Traverso", Faculty of Sciences MM.FF.NN, University of Pavia, (Supervisor: Dr. Maria Rosalia Pasca).
2008 Thesis internship at the Laboratory of Molecular Microbiology, Department of Cell and Developmental Biology, Faculty of Sciences MM.FF.NN, University of Palermo, (Supervisor: Prof. Anna Maria Puglia).
TECHNICAL SKILLS
Microbiological techniques: Preparation of solid and liquid culture media; techniques of sterilization (autoclave, oven, UV); preparation of bacterial cultures (E. coli, S. coelicolor, M. smegmatis); methods for determining the antimicrobial activity of different antibiotics; preparation of competent cells and transformation of E. coli and M. smegmatis using electroporation.
Molecular biology techniques: extraction of plasmidic DNA; extraction of genomic DNA from enteric bacteria and mycobacteria; extraction of total RNA from mycobacteria; DNA digestion with restriction enzymes; cloning techniques; PCR amplification; Southern blotting; Redirect-technology; construction of a genomic library; Retrotranscription-PCR, Real-Time PCR using: intercalating dyes (e.g. SYBR Green) and probes specific for a fragment (e.g. Dual-labeled probes, TaqMan assay).
Bioinformatics techniques: molecular phylogenetic analysis within the genus Mycobacterium, multiple sequence alignment, identification of orthologous genes and evaluation of the degree of homology.
Bioinformatic analysis using the following programs or sites: Eagle view, ClustalW, EXPASY proteomics server, MycoBrowser, SIB Microbe Browser, Sanger istitute mycobacterium blast server.
Good knowledge of the software:
Microsoft Windows XP/Vista, Microsoft Office 2007 Word/Excel/PowerPoint/Publisher.
Knowledge of various graphic programs: Adobe Photoshop, Corel PaintShop Photo.
FOREIGN LANGUAGES
English good knowledge
French basic knowledge
